| 2025 Tolentino Open | |
|---|---|
| Date | 15–21 September 2025 |
| Edition | 1st |
| Category | WTA 125 |
| Prize money | €100,000 |
| Surface | Clay |
| Location | Tolentino, Italy |
| Champions | |
| Singles | |
 Oleksandra Oliynykova | |
| Doubles | |
 Jesika Malečková / Miriam Škoch | |
The 2025 Lexus Tolentino Open was a professional women's tennis tournament played on outdoor clay courts. It was the first edition of the tournament and part of the 2025 WTA 125 tournaments. It took place in Tolentino, Italy between 15 and 21 September 2025. [1] [2]
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 EGY | Mayar Sherif | 103 | 1 |
 LAT | Darja Semeņistaja | 110 | 2 |
| CZE | Sára Bejlek | 113 | 3 |
 SUI | |||
 AUT | Sinja Kraus | 128 | 5 |
 NED | Arantxa Rus | 135 | 6 |
| CZE | Dominika Šalková | 147 | 7 |
 SLO | Tamara Zidanšek | 150 | 8 |
| SLO | Kaja Juvan | 154 | 9 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry as lucky losers:
Carson Branstine → replaced by 
Noma Noha Akugue Simona Waltert → replaced by 
Aurora Zantedeschi | Country | Player | Country | Player | Rank | Seed |
|---|---|---|---|---|---|
| CZE | Jesika Malečková | CZE | Miriam Škoch | 176 | 1 |
 GBR | Alicia Barnett |  FRA | Elixane Lechemia | 249 | 2 |
| Amina Anshba | GBR | Eden Silva | 305 | 3 | |
| ITA | Angelica Moratelli | ITA | Aurora Zantedeschi | 343 | 4 |
Oleksandra Oliynykova def. Nuria Brancaccio 6–2, 6–0 Jesika Malečková / Miriam Škoch def. Silvia Ambrosio / Nuria Brancaccio, 6–3, 3–6, [10–8]
