| Seat number | Senator | Party | Term | |
|---|
| From | To | Notes |
|---|
| President of the Senate | José Mujica | | Broad Front | 15 February 2020 | 1 March 2020 | President from the beginning of the legislature until the inauguration of the Vice President. |
| Beatriz Argimón | | National Party | 1 March 2020 | 1 March 2025 | She took office as Vice President of the Republic. |
| 1st. | José Mujica | | Broad Front | 1 March 2020 | 20 October 2020 | He resigned due to suffering from an autoimmune disease and the COVID-19 pandemic. [14] |
| Alejandro Sánchez | 20 October 2020 | 15 February 2025 | |
| 2nd | Lucía Topolansky | | Broad Front | 15 February 2020 | 1 March 2022 | Resigned the seat to retire |
| Sebastián Sabini | 2 March 2022 | | Assumes seat after Topolansky's retirement |
| 3rd | Eduardo Bonomi | | Broad Front | 15 February 2020 | 20 February 2022 | Died while in office |
| Daniel Caggiani | 2 March 2022 | | |
| 4th | Sandra Lazo | | Broad Front | 15 February 2020 | 15 February 2025 | |
| 5th | Charles Carrera | | Broad Front | 15 February 2020 | 25 September 2024 | He resigns in response to a request from the Public Prosecutor's Office to remove his immunity due to an ongoing investigation against him |
| 6th | Mario Bergara | | Broad Front | 15 February 2020 | 15 February 2025 | |
| 7th | Liliam Kechichián | | Broad Front | 15 February 2020 | 15 February 2025 | |
| 8th | Enrique Rubio | | Broad Front | 15 February 2020 | 5 December 2023 | |
| 9th | Amanda Della Ventura | | Broad Front | 15 February 2020 | 15 February 2025 | |
| 10th | Óscar Andrade | | Broad Front | 15 February 2020 | 15 February 2025 | |
| 11th | Carolina Cosse | | Broad Front | 15 February 2020 | 20 November 2020 | She resigned her seat to take office as Intendant of Montevideo |
| Silvia Nane | 20 November 2020 | 15 February 2025 | |
| 12th | Daniel Olesker | | Broad Front | 15 February 2020 | 6 December 2022 | He resigned to serve as chairman of the Social Affairs Committee of the Broad Front |
| José Nunes | 5 December 2022 | 15 February 2025 | |
|
| 13th | José Carlos Mahía | | Broad Front | 15 February 2020 | 1 March 2020 | He assumes as first substitute while Danilo Astori serves as Minister of Economy. |
| Danilo Astori | 1 March 2020 | 15 November 2020 | |
|
| José Carlos Mahía | 15 November 2020 | 15 February 2025 | Resigned the seat to retire |
| 14th | Carmen Asiaín | | National Party | 15 February 2020 | 15 February 2025 | The holder was Luis Lacalle Pou but he took office as President of the Republic. The first substitute; Falero resigned when he was appointed Deputy Director of the Office of Planning and Budget. |
| 15th | Álvaro Delgado | | National Party | 15 February 2020 | 1 March 2020 | He resigned when he was appointed Secretary of the Presidency of Uruguay. Amin Niffouri took over. |
| Amin Niffouri | 1 March 2020 | 15 February 2025 | |
| 16th | Graciela Bianchi | | National Party | 15 February 2020 | 15 February 2025 | |
| 17th | Luis Alberto Heber | | National Party | 15 February 2020 | 1 March 2020 | He resigned when he was appointed Minister of Transport and Public Works. |
| Gustavo Penadés | 1 March 2020 | 11 October 2023 | He was stripped of his parliamentary immunity by the Senate due to a criminal investigation against him. |
| 18th | Sergio Botana | | National Party | 15 February 2020 | 15 February 2025 | |
| 19th | Gloria Rodríguez Santo | | National Party | 15 February 2020 | 15 February 2025 | |
| 20th | Javier García | | National Party | 15 February 2020 | 1 March 2020 | He resigned when he was appointed Minister of National Defense. |
| Sergio Abreu | | National Party | 1 March 2020 | 15 February 2025 | |
| 21st | Jorge Larrañaga | | National Party | 15 February 2020 | 1 March 2020 | He resigned when he was appointed Minister of the Interior. |
| Carlos Camy | National Party | 1 March 2020 | 15 February 2025 | |
| 22nd | Jorge Gandini | | National Party | 15 February 2020 | 15 February 2025 | |
| 23rd | Juan Sartori | | National Party | 15 February 2020 | 15 February 2025 | |
| 24th | Ernesto Talvi | | Colorado Party | 15 February 2020 | 1 March 2020 | He resigned when he was appointed Minister of the Foreign Relations. |
| Carmen Sanguinetti | 1 March 2020 | 15 February 2025 | |
| 25th | Adrián Peña | | Colorado Party | 15 February 2020 | 26 August 2020 | He resigned when he was appointed Minister of Environment. |
| Pablo Lanz | 27 August 2020 | 15 February 2025 | |
| 26th | Julio María Sanguinetti | | Colorado Party | 15 February 2020 | 20 October 2020 | He resigned his seat. [15] |
| Tabaré Viera | Colorado Party | 20 October 2020 | 23 August 2021 | He resigned when he was appointed Minister of Tourism |
| Raúl Batlle Lamuraglia | 23 August 2021 | 15 February 2025 | |
| 27th | Germán Coutinho | | Colorado Party | 15 February 2020 | 15 February 2025 | |
| 28th | Guido Manini Ríos | | Open Cabildo | 15 February 2020 | 15 February 2025 | |
| 29th | Guillermo Domenech | | Open Cabildo | 15 February 2020 | 15 February 2025 | |
| 30th | Irene Moreira | | Open Cabildo | 15 February 2020 | 1 March 2020 | She resigned when he was appointed Minister of Housing, Territorial Planning and Environment of Uruguay . |
| Raúl Lozano | 1 March 2020 | 5 May 2023 | He was appointed Minister of Housing and Territorial Planning after the resignation of Irene Moreira |
| Irene Moreira | 8 May 2023 | 15 February 2025 | After her resignation from the Ministry of Housing and Territorial Planning, she returned to her seat |
| Source: Members of the Senate |