| Seat number | Senator | Party | Term | |
|---|
| From | To | Notes |
|---|
| President of the Senate | Alejandro Sánchez | | Broad Front | 15 February 2025 | 1 March 2025 | President from the beginning of the legislature until the inauguration of the Vice President. |
| Carolina Cosse | 1 March 2025 | | She took office as Vice President of the Republic. |
|
| 1st. | Alejandro Sánchez | | Broad Front | — | As the top senator of the leading party, he temporarily presided over the Senate until Vice President Cosse's inauguration, then became Secretary of the Presidency. |
| Nicolás Viera | | Broad Front | 15 February 2025 | | Assumed seat as first alternate. |
| 2nd | Blanca Rodríguez | | Broad Front | 15 February 2025 | | |
| 3rd | Cristina Lustemberg | | Broad Front | 15 February 2025 | 1 March 2025 | Resigned to become Minister of Public Health. |
| Patricia Kramer | 1 March 2025 | | Assumed seat as first alternate. |
| 4th | Daniel Caggiani | | Broad Front | 15 February 2025 | | |
| 5th | Sandra Lazo | | Broad Front | 15 February 2025 | 1 March 2025 | Resigned to become Minister of National Defense. |
| Pedro Irigoin | 1 March 2025 | 10 July 2025 | Assumed seat as first alternate; later resigned to become Secretary General of the Intendancy of Canelones. |
| Zulimar Ferreira | 10 July 2025 | | Assumed seat as second alternate. |
| 6th | Sebastián Sabini | | Broad Front | 15 February 2025 | | |
| 7th | Cecilia Cairo | | Broad Front | — | Resigned to become Minister of Housing and Territorial Planning of Uruguay. |
| Bettiana Díaz | 15 February 2025 | | Assumed seat as first alternate. |
| 8th | Alfredo Fratti | | Broad Front | 15 February 2025 | 1 March 2025 | Resigned to become Minister of Livestock, Agriculture and Fisheries. |
| Eduardo Antonini | 1 March 2025 | | Assumed seat as first alternate. |
| 9th | Lucía Etcheverry | | Broad Front | 15 February 2025 | 1 March 2025 | Resigned to become Minister of Transport and Public Works. |
| Aníbal Pereyra | 1 March 2025 | | Assumed seat as first alternate. |
| 10th | Óscar Andrade | | Broad Front | 15 February 2025 | | |
| 11th | Constanza Moreira | | Broad Front | 15 February 2025 | | |
| 12th | Mario Bergara | | Broad Front | 15 February 2025 | 8 July 2025 | Resigned to take office as Intendant of Montevideo. |
| Liliam Kechichián | 30 June 2025 | | Assumed seat as first alternate. |
| 13th | Felipe Carballo Da Costa | | Broad Front | 15 February 2025 | | |
| 14th | Gonzalo Civila | | Broad Front | 15 February 2025 | 1 March 2025 | Resigned to become Minister of Social Development. |
| Gustavo González Soto | 1 March 2025 | | Assumed seat as first alternate. |
| 15th | Edgardo Ortuño | | Broad Front | 15 February 2025 | 1 March 2025 | Resigned to become Minister of Environment. |
| Eduardo Brenta | 1 March 2025 | | Assumed seat as first alternate. |
| 16th | Carolina Cosse | | Broad Front | — | Did not assume seat. |
| Silvia Nane | 15 February 2025 | 10 July 2025 | Assumed seat as first alternate; later resigned to become Director of Sustainable and Smart Development of the Intendancy of Montevideo. |
| Daniel Borbonet | 10 July 2025 | | Assumed seat as second alternate. |
| 17th | Javier García Duchini | | National Party | 15 February 2025 | | |
| 18th | Sergio Botana | | National Party | 15 February 2025 | | |
| 19th | María Fajardo | | National Party | 15 February 2025 | | |
| 20th | Sebastián da Silva | | National Party | 15 February 2025 | | |
| 21st | Luis Lacalle Pou | | National Party | — | The 2020–2025 president resigned from his seat after being elected. |
| Martín Lema | 15 February 2025 | | Assumed seat as first alternate. |
| 22nd | Álvaro Delgado | | National Party | 15 February 2025 | 5 August 2025 | Resigned to become President of the National Party |
| José Luis Falero | 8 July 2025 | | Assumed seat as first alternate. |
| 23rd | Graciela Bianchi | | National Party | 15 February 2025 | | |
| 24th | Nicolas Olivera Seiguerman | | National Party | 15 February 2025 | 8 July 2025 | Resigned to take office as Intendant of Paysandú. |
| Carlos Moreira | 15 July 2025 | | Assumed seat as first alternate. |
| 25th | Luis Alberto Héber | | National Party | 15 February 2025 | 14 October 2025 | Resigned upon retirement. |
| Rodrigo Blás | 14 October 2025 | | Assumed seat as first alternate. |
| 26th | Andrés Ojeda | | Colorado Party | 15 February 2025 | | |
| 27th | Gustavo Zubía | | Colorado Party | 15 February 2025 | | |
| 28th | Pedro Bordaberry | | Colorado Party | 15 February 2025 | | |
| 29th | Tabaré Viera | | Colorado Party | 15 February 2025 | | |
| 30th | Robert Silva | | Colorado Party | 15 February 2020 | | |
| Source: Members of the Senate |