DEAE-Sepharose

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DEAE-Sepharose
Names
IUPAC name
Sepharose CL 6B, 2-(diethylamino)ethyl ether
Identifiers
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UNII
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

DEAE-Sepharose is a tradename for the anion-exchange reactive group, diethylaminoethanol (DEAE) covalently linked to Sepharose (a polysaccharide polymer). [1]

Ion exchange

Ion exchange is an exchange of ions between two electrolytes or between an electrolyte solution and a complex. In most cases the term is used to denote the processes of purification, separation, and decontamination of aqueous and other ion-containing solutions with solid polymeric or mineralic "ion exchangers".

Sepharose is a tradename for a crosslinked, beaded-form of agarose, a polysaccharide polymer material extracted from seaweed. Its brand name is derived from Separation-Pharmacia-Agarose. A common application for the material is in chromatographic separations of biomolecules.

Polysaccharide polymeric carbohydrate molecules composed of long chains of monosaccharide units bound together by glycosidic linkages and on hydrolysis give the constituent monosaccharides or oligosaccharides

Polysaccharides are polymeric carbohydrate molecules composed of long chains of monosaccharide units bound together by glycosidic linkages, and on hydrolysis give the constituent monosaccharides or oligosaccharides. They range in structure from linear to highly branched. Examples include storage polysaccharides such as starch and glycogen, and structural polysaccharides such as cellulose and chitin.

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Brine A highly concentrated solution of a salt in water

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Matronae Aufaniae

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In ancient Roman religion, the Furrinalia was an annual festival held on 25 July to celebrate the rites (sacra) of the goddess Furrina. Varro notes that the festival was a public holiday (feriae publicae dies). Both the festival and the goddess had become obscure even to the Romans of the Late Republic; Varro notes that few people in his day even know her name. One of the fifteen flamines was assigned to her, indicating her archaic stature, and she had a sacred grove (lucus) on the Janiculum, which may have been the location of the festival. Furrina was associated with water, and the Furrinalia follows the Lucaria on 19 and 21 July and the Neptunalia on 23 July, a grouping that may reflect a concern for summer drought.

Nickel(II) chloride chemical compound

Nickel(II) chloride (or just nickel chloride), is the chemical compound NiCl2. The anhydrous salt is yellow, but the more familiar hydrate NiCl2·6H2O is green. Nickel(II) chloride, in various forms, is the most important source of nickel for chemical synthesis. The nickel chlorides are deliquescent, absorbing moisture from the air to form a solution. Nickel salts have been shown to be carcinogenic to the lungs and nasal passages in cases of long-term inhalation exposure.

Ion chromatography

Ion chromatography is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids. However, ion chromatography must be done in conditions that are one unit away from the isoelectric point of a protein.

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A salt metathesis reaction, sometimes called a double replacement reaction, double displacement reaction, or double decomposition reaction, is a chemical process involving the exchange of bonds between two non-reacting chemical species which results in the creation of products with similar or identical bonding affiliations. This reaction is represented by the general scheme:

Titanium(III) chloride is the inorganic compound with the formula TiCl3. At least four distinct species have this formula; additionally hydrated derivatives are known. TiCl3 is one of the most common halides of titanium and is an important catalyst for the manufacture of polyolefins.

Molybdenum(II) chloride chemical compound

Molybdenum dichloride describes chemical compounds with the empirical formula MoCl2. At least two forms are known, and both have attracted much attention from academic researchers because of the unexpected structures seen for these compounds and the fact that they give rise to hundreds of derivatives. The form discussed here is Mo6Cl12. The other molybdenum(II) chloride is potassium octachlorodimolybdate.

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Protein C inhibitor protein-coding gene in the species Homo sapiens

Protein C inhibitor is a serine protease inhibitor (serpin) which limits the activity of protein C.

Diethylaminoethyl cellulose chemical compound

Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids. Gel matrix beads are derivatized with diethylaminoethanol (DEAE) and lock negatively charged proteins or nucleic acids into the matrix. The proteins are released from the resin by increasing the salt concentration of the solvent or changing the pH of the solution as to change the charge on the protein.

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CLCN7 protein-coding gene in the species Homo sapiens

Chloride channel 7 alpha subunit also known as H+/Cl exchange transporter 7 is a protein that in humans is encoded by the CLCN7 gene. In melanocytic cells this gene is regulated by the Microphthalmia-associated transcription factor.

SLC4A3 protein-coding gene in the species Homo sapiens

Anion exchange protein 3 is a membrane transport protein that in humans is encoded by the SLC4A3 gene. AE3 is functionally similar to the Band 3 Cl/HCO3 exchange protein but it is expressed primarily in brain neurons and in the heart. Like AE2 its activity is sensitive to pH. AE3 mutations have been linked to seizures.

Diethylethanolamine chemical compound

Diethylethanolamine (DEAE) is a chemical compound with the molecular formula C6H15NO. It is used as a precursor in the production of a variety of chemical commodities such as the local anesthetic procaine. It can be reacted with 4-aminobenzoic acid to make procaine. DEAE can be used as a precursor for DEAE-cellulose resin, which is commonly used in ion exchange chromatography. DEAE can also be conveniently obtained from renewable sources. It is chemically stable and able to absorb carbon dioxide (CO2) from its surroundings. In solution, it can decrease the surface tension of water when the temperature is increased.

Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). In solution, the resin is coated with positively charged counter-ions (cations). Anion exchange resins will bind to negatively charged molecules, displacing the counter-ion. Anion exchange chromatography is commonly used to purify proteins, amino acids, sugars/carbohydrates and other acidic substances with a negative charge at higher pH levels. The tightness of the binding between the substance and the resin is based on the strength of the negative charge of the substance.

Harrison Paul Davis III is a former American football wide receiver in the National Football League who played for the San Diego Chargers. He played college football for the Virginia Cavaliers.

References

  1. Borras-Cuesta F, Truche E (October 1984). "The purification of myosin long subfragment 2 by DEAE-Sepharose CL-6B ion-exchange chromatography". Anal. Biochem. 142 (1): 84–7. doi:10.1016/0003-2697(84)90519-0. PMID   6393822.

Medical Subject Headings (MeSH) is a comprehensive controlled vocabulary for the purpose of indexing journal articles and books in the life sciences; it serves as a thesaurus that facilitates searching. Created and updated by the United States National Library of Medicine (NLM), it is used by the MEDLINE/PubMed article database and by NLM's catalog of book holdings. MeSH is also used by ClinicalTrials.gov registry to classify which diseases are studied by trials registered in ClinicalTrials.