DisProt

Last updated
DisProt
DisProt 8 logo.svg
Content
DescriptionManually curated database of Intrinsically Disordered Proteins (IDPs) and regions (IDRs)
Data types
captured
Intrinsically Disordered Proteins
Organisms all
Contact
LaboratoryBioComputing UP laboratory (Dept. of Biomedical Sciences, University of Padova)
Primary citation PMID   34850135
Access
Website https://disprot.org/
Download URL https://disprot.org/download
Miscellaneous
License Creative Commons Attribution 4.0 International (CC BY 4.0) License
Curation policyManual curation from professional and community biocurators

DisProt is a manually curated biological database of intrinsically disordered proteins (IDPs) and regions (IDRs). [1] [2] [3] DisProt annotations cover state information on the protein but also, when available, its state transitions, interactions and functional aspects of disorder detected by specific experimental methods. DisProt is hosted and maintained in the BioComputing UP laboratory (Dept. of Biomedical Sciences, University of Padua).

Contents

Website

The latest DisProt version, DisProt 9, [3] includes more than 2300 protein entries and more than 4500 pieces of evidence of structural state, state transitions, interactions and functions, along with more than 2500 scientific publications annotated.

Biocuration in DisProt

DisProt entries are annotated by professional and community biocurators from experimental data published in scientific literature. The DisProt home page features examples of DisProt entries, i.e. p53 and Catenin beta-1, along with entries of proteins belonging to the SARS-CoV-2 virus, e.g. Nucleoprotein.

DisProt 9 DisProt 9.0.1 homepage.png
DisProt 9

Thematic datasets

Starting 2020, DisProt releases ‘thematic datasets’ describing biological areas where IDPs are involved in and play a crucial role. [3] All the entries belonging to these datasets are tagged with the name of the theme.

  • Unicellular toxins and antitoxins (DisProt release 2020_12)
  • Extracellular matrix proteins (DisProt release 2021_06)
  • Viral proteins (DisProt release 2021_12)

Model organism entries

In the DisProt home page model organisms are represented by an icon, the name of the species and the number of DisProt entries belonging to each specific organism. Entries from the following organisms are accessible from the DisProt home page under the ‘Organisms’ section and can be downloaded as single files: Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomices cerevisiae, Escherichia coli, Arabidopsis thaliana, Drosophila melanogaster, Caenorhabditis elegans.

DisProt versions and releases

DisProt versions and releases include changes to the website and to the manually curated content of the database.

DisProt ontologies

DisProt uses three different ontologies to annotate disordered regions, the Intrinsically Disordered Proteins Ontology (IDPO), the Evidence and Conclusion Ontology (ECO) and the Gene Ontology (GO). DisProt has a dedicated page for each IDPO term that include the identifier, name and definition of the term and cross-references to external ontologies, e.g. Gene Ontology. Each IDPO term page list all the DisProt entries annotated with that specific term.

References

  1. Vucetic, Slobodan; Obradovic, Zoran; Vacic, Vladimir; Radivojac, Predrag; Peng, Kang; Iakoucheva, Lilia M.; Cortese, Marc S.; Lawson, J. David; Brown, Celeste J. (2005-01-01). "DisProt: a database of protein disorder". Bioinformatics. 21 (1): 137–140. doi: 10.1093/bioinformatics/bth476 . ISSN   1367-4803. PMID   15310560.
  2. Sickmeier, Megan; Hamilton, Justin A.; LeGall, Tanguy; Vacic, Vladimir; Cortese, Marc S.; Tantos, Agnes; Szabo, Beata; Tompa, Peter; Chen, Jake (2007-01-01). "DisProt: the Database of Disordered Proteins". Nucleic Acids Research. 35 (Database issue): D786–793. doi:10.1093/nar/gkl893. ISSN   1362-4962. PMC   1751543 . PMID   17145717.
  3. 1 2 3 4 Quaglia, Federica; Mészáros, Bálint; Salladini, Edoardo; Hatos, András; Pancsa, Rita; Chemes, Lucía B.; Pajkos, Mátyás; Lazar, Tamas; Peña-Díaz, Samuel; Santos, Jaime; Ács, Veronika (2021-11-25). "DisProt in 2022: improved quality and accessibility of protein intrinsic disorder annotation". Nucleic Acids Research. 50 (D1): D480 –D487. doi:10.1093/nar/gkab1082. ISSN   1362-4962. PMC   8728214 . PMID   34850135.
  4. Piovesan, Damiano; Tabaro, Francesco; Mičetić, Ivan; Necci, Marco; Quaglia, Federica; Oldfield, Christopher J.; Aspromonte, Maria Cristina; Davey, Norman E.; Davidović, Radoslav (2016-11-28). "DisProt 7.0: a major update of the database of disordered proteins". Nucleic Acids Research. 45 (D1): D219 –D227. doi:10.1093/nar/gkw1056. ISSN   1362-4962. PMC   5210544 . PMID   27899601.
  5. Hatos, András; Hajdu-Soltész, Borbála; Monzon, Alexander M.; Palopoli, Nicolas; Álvarez, Lucía; Aykac-Fas, Burcu; Bassot, Claudio; Benítez, Guillermo I.; Bevilacqua, Martina; Chasapi, Anastasia; Chemes, Lucia (2019). "DisProt: intrinsic protein disorder annotation in 2020". Nucleic Acids Research. 48 (D1): D269 –D276. doi: 10.1093/nar/gkz975 . PMC   7145575 . PMID   31713636.
  6. Kovačević JJ (June 2012). "Computational analysis of position-dependent disorder content in DisProt database". Genomics Proteomics Bioinformatics. 10 (3): 158–65. doi:10.1016/j.gpb.2012.01.002. PMC   5056116 . PMID   22917189.