Glowing Plant project

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The Glowing Plant project was the first crowdfunding campaign for a synthetic biology application. The project was started by the Sunnyvale-based hackerspace Biocurious as part of the DIYbio philosophy. According to the project's goals, funds were used to create a glowing Arabidopsis thaliana plant using firefly luminescence genes. Long-term ambitions (never realized) included the development of glowing trees that can be used to replace street lights, reducing CO2 emissions by not requiring electricity.

Contents

In 2023 this concept was finally achieved, commercialized, and brought to market by Light Bio, [1] who used a fungal luminescence gene in petunias to achieve the first commercially available luminescent plants. These are currently only available in the US (as of 2024)

Project funding

Using Kickstarter, the project's founders raised $484,000 on June 8, 2013. [2] This was significantly more than the initial target of $65,000.

Seeds were initially scheduled to be delivered in April 2014, and subsequently scheduled for the fall of 2014. [3] In March 2016, delivery of seeds was forecast for 2016 on the Glowing Plant website. The company encountered difficulty in producing plants that emit significant amounts of light, resulting in a transition to producing moss that emits a patchouli scent. [4] They later announced via email December 2017 that the company was permanently ceasing operations.

Methods

Biocurious planned to tweak the biobrick containing six genes, including luciferin-regenerating enzyme and luciferase from fireflies. [5] [6] During initial development, they would use Agrobacterium to test the transfer of the genetic circuit. When producing the final product, they intended to instead use a gene gun to avoid issues related to regulation of GM plants. [4] Over the course of the project, several plants were mentioned as being recipients, including Arabidopsis thaliana, Nicotiana tabacum , and roses. [6] Issues surrounding the production included the difficulty of moving the six component genes of the metabolic pathway, increasing the dim light produced by the plant following insertion, and preventing the pathway from being silenced. [4]

Controversy

The project generated widespread media attention and a discussion of appropriate uses of biotechnology. [7] As a result of the controversy, Kickstarter decided to prohibit genetically modified organisms as rewards to project backers. [8]

Though the Animal and Plant Health Inspection Service (APHIS) has shown no regulatory concerns about the project, some synthetic biologists and policy researchers have questioned the project's feasibility and impact on future oversight or public opinion of synthetic biology. In particular, if the company were to encourage backers to use a genetic DIY kit themselves, additional regulatory oversight would likely occur. [9]

See also

Related Research Articles

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Arabidopsis thaliana, the thale cress, mouse-ear cress or arabidopsis, is a small plant from the mustard family (Brassicaceae), native to Eurasia and Africa. Commonly found along the shoulders of roads and in disturbed land, it is generally considered a weed.

<span class="mw-page-title-main">Bioluminescence</span> Emission of light by a living organism

Bioluminescence is the production and emission of light by living organisms. It is a form of chemiluminescence. Bioluminescence occurs widely in marine vertebrates and invertebrates, as well as in some fungi, microorganisms including some bioluminescent bacteria, and terrestrial arthropods such as fireflies. In some animals, the light is bacteriogenic, produced by symbiotic bacteria such as those from the genus Vibrio; in others, it is autogenic, produced by the animals themselves.

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<span class="mw-page-title-main">Chemiluminescence</span> Emission of light as a result of a chemical reaction

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Cambrian Genomics was a biotechnology company based in San Francisco which used a laser-based technique to synthesize DNA. Cambrian Genomics produced genetic material for a 2013 effort to produce genetically modified glowing plants for sale to the public. A Kickstarter campaign raised $500,000 for the Glowing Plant project.

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RNA-directed DNA methylation (RdDM) is a biological process in which non-coding RNA molecules direct the addition of DNA methylation to specific DNA sequences. The RdDM pathway is unique to plants, although other mechanisms of RNA-directed chromatin modification have also been described in fungi and animals. To date, the RdDM pathway is best characterized within angiosperms, and particularly within the model plant Arabidopsis thaliana. However, conserved RdDM pathway components and associated small RNAs (sRNAs) have also been found in other groups of plants, such as gymnosperms and ferns. The RdDM pathway closely resembles other sRNA pathways, particularly the highly conserved RNAi pathway found in fungi, plants, and animals. Both the RdDM and RNAi pathways produce sRNAs and involve conserved Argonaute, Dicer and RNA-dependent RNA polymerase proteins.

References

  1. Glowing Plants: Natural Lighting with no Electricity by Antony Evans — Kickstarter
  2. "Progress to date". Glowing Plant website. Archived from the original on 2014-10-02. Retrieved 2 October 2014.
  3. 1 2 3 "Why Kickstarter's Glowing Plant Left Backers in the Dark". MIT Technology Review. Retrieved 2022-04-04.
  4. "Team:Cambridge/Bioluminescence - 2010.igem.org". 2010.igem.org. Retrieved 2022-04-04.
  5. 1 2 Regalado, Antonio (July 15, 2016). "Why Kickstarter's Glowing Plant Left Backers in the Dark". MIT Technology Review.
  6. Ariana Eunjung Cha (October 5, 2013). "Glowing plants spark environmental debate". The Seattle Times. Archived from the original on October 20, 2014. Retrieved 21 September 2014.
  7. David Holmes (August 2, 2013). "Why did Kickstarter ban GMOs?". PandoDaily. Archived from the original on November 6, 2013. Retrieved October 21, 2013.
  8. Ikemoto, Lisa (2017). DIY Bio: Hacking Life in Biotech's Backyard. UC Davis School of Law. pp. 558–559.