Black Knight (Nathan Garrett) - A descendant of Sir Percy of Scandia and an enemy of Giant-Man who uses an arsenal of medieval weapons that employ modern technology (including a lance that fired bolts of energy) and genetically engineers and creates a winged horse called Aragorn.
Egghead (leader) - A mad scientist with an egg-shaped head who is an enemy of Hank Pym.
Beetle (Abner Jenkins) - A supervillain in beetle-themed armor who is an enemy of Spider-Man.
Moonstone - A supervillain whose powers derive from an alien gem who previously fought Captain America and the Hulk. She served as Egghead's deputy leader of the Masters of Evil.
Black Mamba - A member of the Serpent Society who has Darkforce energy control and illusion-casting abilities. She is an associate who appears as Tanya Sealy.
Powderkeg - A super-strong villain who can sweat a nitroglycerin compound.
Puff Adder - A mutant and Serpent Society member with the power to breathe various debilitating gases (in one instance, the gas was able to eat away a metal lock) and inflate his body mass to a certain extent. He also has superhuman strength and increased physical durability.
Aqueduct - A supervillain who can control and shape water.
Bison - A bison-themed supervillain who was transformed from an injured basketball player by the Egyptian god Seth and had previously fought Luke Cage and Thunderstrike.
Boomerang - A boomerang-wielding supervillain and an enemy of Spider-Man.
Cardinal - A supervillain who wears an armored suit that can fly and has a number of offensive weapons including energy blasters, a grenade launcher and a tar gun.
Cyclone (Pierre Fresson) - A supervillain who wears a costume that enables him to create tornado-force whirlwinds.
Dragonfly - A dragonfly-themed supervillain and former Maggia member who can fly and has a hypnotic gaze.
Eel (Edward Lavell) - A supervillain who wears an electrified lubricated suit.
Flying Tiger - A supervillain whose tiger-like body armor makes him capable of powered flight, as well as giving him enhanced strength, durability and endurance, and a set of claws.
Dark Phoenix - An unidentified golden-skinned female host of the Phoenix Force who wears a dark robe. Her appearance is revealed to be the form of a Mystique variant from Earth-14412.
Berserkers - The pets of Dark Phoenix who consider her their mother.
Hound - An unidentified alternate reality version of Wolverine who is loyal to Dark Phoenix and has spikes coming out of his body.
An unidentified reality version of Thor.
Ghost Goblin - A version of Green Goblin from an unidentified reality with the powers of Ghost Rider who can also throw flaming skull-shaped Noggin Bombs.
Kid Thanos - A younger version of Thanos who was apparently taken from the past.
In One World Under Doom #2, the following villains were identified as the Masters of Evil when they agreed to help the Avengers fight Doctor Doom at the time when he was the Sorcerer Supreme:
Arcade - A criminal genius who has made use of his Murderworld locations.
Baron Mordo - A sorcerer who is an enemy of Doctor Strange.
Mysterio - An illusionist and enemy of Spider-Man.
In Avengers Vol. 9 #25, another Masters of Evil group led a plot to take over the Impossible City to make it their own nation and consisted of the following villains:
Dreadknight - A Latverian engineer who had a helmet bio-fused to his head by Doctor Doom's men using the Bio-Fusor for conspiring against Doom and became a knight-themed villain with his steed Hellhorse.
Exterminatrix - A villain who utilizes gold-transforming bullets made from the blood of her late adoptive father Doctor Midas.
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