This is the progression of world record improvements of the 400 metres W80 division of Masters athletics.
- Key
Incomplete information Pending better indoor records
| Hand | Auto | Athlete | Nationality | Birthdate | Age | Location | Date | Ref |
|---|---|---|---|---|---|---|---|---|
| 1:28.63 | Marjorie Allison |  Australia | 13 September 1944 | 80 years, 360 days | Brisbane | 8 September 2025 | [1] | |
| 1:29.84 | Rietje Dijkman |  Netherlands | 21 June 1939 | 80 years, 80 days | Caorle | 9 September 2019 | ||
| 1:31.10 i | Emma Mazzenga |  Italy | 1 August 1933 | 80 years, 241 days | Budapest | 30 March 2014 | ||
| 1:31.21 | Emma Mazzenga | Italy | 1 August 1933 | 80 years, 31 days | Padua | 1 September 2013 | ||
| 1:39.99 | Christine Du Plooy |  South Africa | 25 March 1931 | Kings Park | 29 April 2011 | |||
| 1:40.45 | Polly Clarke |  United States | 17 July 1910 | Indianapolis | 3 August 1990 |