Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels. Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar electrokinetic chromatography (MEKC) belong also to this class of methods. In CE methods, analytes migrate through electrolyte solutions under the influence of an electric field. Analytes can be separated according to ionic mobility and/or partitioning into an alternate phase via non-covalent interactions. Additionally, analytes may be concentrated or "focused" by means of gradients in conductivity and pH.
Richard Neil Zare is the Marguerite Blake Wilbur Professor in Natural Science and a Professor of Chemistry at Stanford University. Throughout his career, Zare has made a considerable impact in physical chemistry and analytical chemistry, particularly through the development of laser-induced fluorescence (LIF) and the study of chemical reactions at the molecular and nanoscale level. LIF is an extremely sensitive technique with applications ranging from analytical chemistry and molecular biology to astrophysics. One of its applications was the sequencing of the human genome.
Csaba Horváth was a Hungarian-American chemical engineer, particularly noted for building the first high-performance liquid chromatograph.
David E. Clemmer is an analytical chemist and the Distinguished Professor and Robert and Marjorie Mann Chair of Chemistry at Indiana University in Bloomington, Indiana, where he leads the Clemmer Group. Clemmer develops new scientific instruments for ion mobility mass spectrometry (IMS/MS), including the first instrument for nested ion-mobility time-of-flight mass spectrometry. He has received a number of awards, including the Biemann Medal in 2006 "for his pioneering contributions to the integration of ion mobility separations with a variety of mass spectrometry technologies."
Capillary electrophoresis–mass spectrometry (CE–MS) is an analytical chemistry technique formed by the combination of the liquid separation process of capillary electrophoresis with mass spectrometry. CE–MS combines advantages of both CE and MS to provide high separation efficiency and molecular mass information in a single analysis. It has high resolving power and sensitivity, requires minimal volume and can analyze at high speed. Ions are typically formed by electrospray ionization, but they can also be formed by matrix-assisted laser desorption/ionization or other ionization techniques. It has applications in basic research in proteomics and quantitative analysis of biomolecules as well as in clinical medicine. Since its introduction in 1987, new developments and applications have made CE-MS a powerful separation and identification technique. Use of CE–MS has increased for protein and peptides analysis and other biomolecules. However, the development of online CE–MS is not without challenges. Understanding of CE, the interface setup, ionization technique and mass detection system is important to tackle problems while coupling capillary electrophoresis to mass spectrometry.
John Calvin Giddings was a Distinguished Professor of chemistry at the University of Utah. Giddings received a B.S. degree from Brigham Young University in 1952 and a PhD from the University of Utah in 1954. Following postdoctoral work at the University of Utah and the University of Wisconsin, he joined the faculty of the University of Utah as assistant professor of chemistry in 1957. He became associate professor in 1959, research professor in 1962, and professor in 1966. Giddings authored or co-authored more than 400 publications and edited 32 books in the field of chemistry. He was executive editor of the journal Separation Science and Technology, and the editor of the series Advances in chromatography.
Milton L. Lee is the H. Tracy Hall Professor of Chemistry at Brigham Young University (BYU).
Leslie Stephen Ettre was a Hungarian-American analytical chemist and scientist who was known for his contributions to the field of chromatography, in particular open-tubular gas chromatography, as well as to documentation of the history of chromatography.
Edward S. Yeung is a Chinese-American chemist who studies spectroscopy and chromatography. Yeung is a Distinguished Professor Emeritus at Iowa State University. He was elected as a Fellow of the American Association for the Advancement of Science. He was a founding co-editor of the Annual Review of Analytical Chemistry from 2008 to 2014 and has served on the editorial committees of a number of other journals.
Richard Dale Smith is a chemist and a Battelle Fellow and chief scientist within the biological sciences division, as well as the director of proteomics research at the Pacific Northwest National Laboratory (PNNL). Smith is also director of the NIH Proteomics Research Resource for Integrative Biology, an adjunct faculty member in the chemistry departments at Washington State University and the University of Utah, and an affiliate faculty member at the University of Idaho and the Department of Molecular Microbiology & Immunology, Oregon Health & Science University. He is the author or co-author of approximately 1100 peer-reviewed publications and has been awarded 70 US patents.
Marek Andrzej Trojanowicz is a Polish chemist, professor of chemical sciences with specialization in analytical chemistry, academic staff member, and head of the Laboratory for Flow Analysis and Chromatography, University of Warsaw, Poland.
Walter Goodrich Jennings was an American chemist, educator and entrepreneur. He was co-founder of J&W Scientific, which became the world's largest supplier of fused silica columns; the company was purchased by Agilent Technologies in 2000.
Daniel Wayne Armstrong is an American chemist who specializes in separation science, chiral molecular recognition, bioanalytic analysis, mass spectrometry and colloid chemistry. He is the Robert A. Welch Distinguished Professor at the University of Texas at Arlington. He has authored ~ 750 publications including 35 book chapters, a book, and holds over 35 patents on separation technologies. He was an associate editor for the prestigious American Chemical Society journal Analytical Chemistry. He is a fellow of the American Chemical Society, Royal Chemical Society (UK), and the National Academy of Inventors. Armstrong has given over 560 invited seminars worldwide at international conferences, universities and corporations. His research and patents formed the basis for two companies: Advanced Separation Technologies, Inc; which was acquires by Sigma-Aldrich Corporation in 2006 and AZYP, LLC in Arlington, TX. His published work has been cited over 50,000 times and his h-index is 115. He is believed to have mentored more graduate level analytical chemists than any living scientist.
John Michael Ramsey is an American analytical chemist at the University of North Carolina at Chapel Hill. He currently holds the position of Minnie N. Goldby Distinguished Professor of Chemistry. His current research with the university focuses on microscale and nanoscale devices such as microchip electrospray, microscale Ion trap mass spectrometers, and microfluidic point of care devices. He is ranked #2 in the "Giants of Nano" field on The Analytical Scientist Power List.
James Wallace Jorgenson is an American academic who previously held the position of William Rand Kenan Jr. Distinguished Professor of Chemistry at UNC-Chapel Hill. He is best known for his work developing capillary zone electrophoresis, and is a member of the American Academy of Arts and Sciences.
Gary M. Hieftje is an analytical chemist, Distinguished Professor, and the Robert & Marjorie Mann Chair of Chemistry at Indiana University in Bloomington, Indiana. Gary M. Hieftje received his A.B. degree at Hope College in Holland, Michigan in 1964, and his PhD from University of Illinois at Urbana–Champaign in 1969. In 1969, he started his career in teaching and research at Indiana University. Hieftje was named a Distinguished Professor in 1985, and entered emeritus status in 2018. As of 2018, Dr. Hieftje has been involved in over 600 publications.
Robert Travis Kennedy is an American chemist specializing in bioanalytical chemistry including liquid chromatography, capillary electrophoresis, and microfluidics. He is currently the Hobart H. Willard Distinguished University Professor of Chemistry and the chair of the department of chemistry at the University of Michigan. He holds joint appointments with the Department of Pharmacology and Department Macromolecular Science and Engineering. Kennedy is an Associate Editor of Analytical Chemistry and ACS Measurement Science AU.
Apryll Marie Stalcup is an Irish chemist who is Professor and was the Director of the Irish Separation Science Cluster at Dublin City University. She was awarded the 2021 Chromatography Forum of the Delaware Valley Stephen Dal Nogare Award, the 2015 American Microchemical Society Benedetti Pichler Award and named in the 2016 Power List of the Top 50 Women Analytical Scientists worldwide. Her research considers surface-confined ionic liquids and chiral separations.
Susan M. Lunte is an American chemist who is the Ralph N. Adams Distinguished Professor of Chemistry and Pharmaceutical Chemistry at the University of Kansas. She also works as director of the NIH COBRE Center for Molecular Analysis of Disease Pathways. She was awarded the 2023 ACS Award in Analytical Chemistry.
Robert E Synovec is an American analytical chemist and professor of chemistry at the University of Washington where he specializes in multidimensional separations and chemometrics. Synovec has received several awards for his contributions to analytical chemistry and separation science, including the GC×GC Scientific Achievement Award and the Marcel E Golay Award, which is given for recognition of a lifetime of achievement in capillary chromatography.
