Mitotic exit

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Mitotic exit is an important transition point that signifies the end of mitosis and the onset of new G1 phase for a cell, and the cell needs to rely on specific control mechanisms to ensure that once it exits mitosis, it never returns to mitosis until it has gone through G1, S, and G2 phases and passed all the necessary checkpoints. Many factors including cyclins, cyclin-dependent kinases (CDKs), ubiquitin ligases, inhibitors of cyclin-dependent kinases, and reversible phosphorylations regulate mitotic exit to ensure that cell cycle events occur in correct order with fewest errors. [1] The end of mitosis is characterized by spindle breakdown, shortened kinetochore microtubules, and pronounced outgrowth of astral (non-kinetochore) microtubules. For a normal eukaryotic cell, mitotic exit is irreversible. [2]

Contents

Proteolytic degradation

Fig. 1 Immunofluorescence patterns of cyclin B and phosphorylated cyclin-dependent kinase1 (Cdk1) in HeLa cells change as they go from G2 to anaphase. Cdk1 and Clb level changes as cell cycle proceeds.jpg
Fig. 1 Immunofluorescence patterns of cyclin B and phosphorylated cyclin-dependent kinase1 (Cdk1) in HeLa cells change as they go from G2 to anaphase.

Many speculations were made with regard to the control mechanisms employed by a cell to promote the irreversibility of mitotic exit in a eukaryotic model organism, the budding yeast Saccharomyces cerevisiae . Proteolytic degradation of cell cycle regulators and corresponding effects on the levels of cyclin-dependent kinases were proposed as a mechanism that promotes eukaryotic cell cycle and metaphase-to-anaphase transition in particular. In this theory, anaphase promoting complex (APC), a class of ubiquitin ligase, facilitates degradation of mitotic cyclins (Clb2) and anaphase-inhibiting factors (PDS1, CUT2) to promote mitotic exit. [3] APC ubiquitinates nine-amino acid motif known as the destruction box (D box) in the NH2-terminal domain of mitotic cyclins for degradation by proteasome. [3] APC in association with Cdc20 (APC-Cdc20) ubiquitinates and targets mitotic cyclins (Clb2) for degradation at initial phase. Simultaneously, APC-Cdc20 mediates degradation of securins, which inhibit separases through binding, at anaphase onset. Released and active separase cleaves cohesin that held sister chromatids together, facilitating separation of sister chromatids and initiates mitotic exit by promoting release of Cdc14 from nucleolus. [4] [5] At later phase, downregulation of Cdk1 and activation of Cdc14, a Cdh1-activating phosphatase, promotes formation of APC in association with Cdh1 (APC-Cdh1) to degrade Clb2s. [2] Cdc20 and Cdh1, which are the activators of APC, recruit substrates such as securin and B-type cyclins(Clb) for ubiquitination. [6] Without Cdk1-Clb2 complexes to phosphorylate proteins that are involved in spindle dynamics such as Sli15, Ase1, and Ask1, spindle elongation and chromosomal segregation are promoted, facilitating mitotic exit. [2] The importance of proteolytic degradation in eukaryotic cell cycle changed the view of cell division as a simple kinase cascade to a more complex process in which interactions among phosphorylation, ubiquitination, and proteolysis are necessary. [3] However, experiments using budding yeast cells with cdc28-as1, an INM-PP1 (ATP analog)-sensitive Cdk allele, proved that destruction of B-type cyclins (Clb) is not necessary for triggering irreversible mitotic exit. [2] Clb2 degradation did shorten the Cdk1-inhibition period required for triggering irreversible mitotic exit indicating that cyclin proteolysis contributes to the dynamic nature of the eukaryotic cell cycle due to slower timescale of its action but is unlikely to be the major determining factor in triggering irreversible cell cycle transitions. [2]

Sic1 levels

Discoveries were made which indicated the importance of the level of the inhibitors of cyclin-dependent kinases in regulating eukaryotic cell cycle. In particular, the level of Sic1, a stoichiometric inhibitor of Clb-CDK complexes in budding yeast, was shown to be particularly important in irreversible G1-S transition by irreversibly activating S phase kinases. [7] Sic1 level was shown to play a major role in triggering irreversible mitotic exit (M-G1 transition) as well as in G1-S transition. During mitosis, decreasing levels of Cdk1 leads to the activation of Cdc14, a phosphatase that counteracts Cdk1 via activation of Cdh1 and Swi5, a transcriptional activator of Sic1 proteins. [8] While degradation of Sic1 to a certain low level triggered the onset of S phase, accumulation of Sic1 to a certain high level was required to trigger irreversible mitotic exit. [2] Cdk1-inhibitors could induce mitotic exit even when degradation of B-type cyclins was blocked by expression of non-degradable Clbs or proteasome inhibitors. However, sister chromatids failed to segregate, and cells reverted to mitosis once the inhibitors were washed away, indicating that a threshold level of the inhibitors needs to be achieved to trigger irreversible mitotic exit independently of cyclin degradations. [9] Despite different thresholds of Sic1 level that are required to trigger mitotic exit compared to G1-S transition, the level of Sic1 was shown to play a key role in regulating eukaryotic cell cycle by inhibiting the activity of CDKs.

Dynamical systems approach

Fig. 2 Irreversible and bistable switch in mitotic exit with control parameter being Sic1 level and order parameter being cell cycle phases. Irreversible Bistable Switch in Mitotic Exit.jpg
Fig. 2 Irreversible and bistable switch in mitotic exit with control parameter being Sic1 level and order parameter being cell cycle phases.

Because eukaryotic cell cycle involves a variety of proteins and regulatory interactions, dynamical systems approach can be taken to simplify a complex biological circuit into a general framework for better analysis. [10] [11] Among the four possible input/output relationships, the relationship between Sic1 level and mitotic exit seems to show the characteristics of an irreversible bistable switch, driven by feedback between APC-Cdh1, Sic1, and Clb2-Cdk1. [2] Bistability is known to control biological functions such as cell cycle control and cellular differentiation and play a key role in many cellular regulatory networks. [12] Bistable input/output relationship is characterized by two stable states with two bifurcation points. Multiple outputs are possible for one specific input in the region of bistability, marked by two bifurcation points. In addition, the bistable relationship displays hysteresis: the final state/output depends on the history of the input as well as the current value of input because the system has a memory. [10] One bifurcation point has a negative control parameter value (the bifurcation point is on the other side of the axis), resulting in disconnection between the two stable states and irreversibility of the transition from one state to the other. With regard to mitotic exit, the two stable states are defined by mitosis and G1 phase. Once Sic1 level (input) accumulates beyond the threshold, irreversible transition occurs from mitosis (stable state I) to G1 phase (stable state II). In the imperfect environment, the only bifurcation that remains intact is saddle-node bifurcation. Saddle-node bifurcation does not break down (saddle-node is the expected generic behavior), while transcritical and pitchfork bifurcations break down in the presence of imperfections. [13] Thus, the only one-dimensional bifurcation that can exist in imperfect biological world is the saddle-node bifurcation. [10] The bistable relation between M-G1 transition and Sic1 level can be represented as a diagram of two saddle-node bifurcations in which the system's behavior changes qualitatively with a small change in control parameter, the amount of Sic1.

Systems-level feedback

Fig. 3 Simplified network involving Cdk1-Clb2, APC-Cdh1, Sic1, and Cdc14. Double negative feedback loop, mediated by APC-Cdh1 and Sic1, is required to suppress Cdk1-Clb2 and trigger mitotic exit. Simplified network controls mitotic exit.jpg
Fig. 3 Simplified network involving Cdk1-Clb2, APC-Cdh1, Sic1, and Cdc14. Double negative feedback loop, mediated by APC-Cdh1 and Sic1, is required to suppress Cdk1-Clb2 and trigger mitotic exit.

Because the behavior of cell cycle critically depends on the amount of Sic1 at the M-G1 transition state, the amount of Sic1 is tightly regulated by systems-level feedbacks. Because Cdk1-Clb2 inhibits Sic1 by phosphorylating Sic1 and making Sic1 available for degradation via ubiquitylation, APC-Cdh1-dependent degradation of Cdk1-Clb2 not only decreases the level of available Cdk1-Clb2 complexes but also increases the level of Sic1 which in turn further inhibits the function of Cdk1-Clb2. [8] This activation of the double negative feedback loop is initiated from APC-Cdc20-dependent degradation of Cdk1-Clb2 and release of Cdc14 from nucleolar protein Net1/Cfi1. [14] FEAR (Cdc14 early anaphase release) pathway facilitates Clb2-Cdk1-dependent phosphorylation of Net1 which transiently releases Cdc14 from Net1. [15] The released Cdc14 and Clb2-Cdk1 complexes go onto form spindles that activates mitotic exit network (MEN). MEN allows sustained release of Cdc14 from the nucleolus, [15] and Cdc14 counters the activity of Clb2-Cdk1 by activating Cdh1 and stabilizing Sic1 through activation of Sic1-transcriptional activator Swi5. [16] Sic1 positively regulates itself by inhibiting Cdk1-Clb2 to release inhibition of Swi5, and Cdh1 also positively regulates itself by inhibiting Clb2-Cdk1 to release inhibition of MEN which can activate Cdc14 and subsequently Cdh1 itself. The double-negative feedback loop, formed by APC-Cdh1 and Sic1, is required to maintain low Clb2-Cdk1 activity because Clb2 auto-activates its synthesis by activating transcriptional factors, Fkh2–Mcm1 Ndd1 complex. [8]

Implications

Eukaryotic cell cycle consists of various checkpoints and feedback loops to ensure faithful and successful cell division. During mitosis for example, when duplicated chromosomes are improperly attached to mitotic spindle, spindle assembly checkpoint (SAC) proteins including Mad and Bub inhibit APC-Cdc20 to delay entry into anaphase and B-type cyclin degradations. In addition, when mitotic spindles are misaligned, MEN and subsequently Cdc14 are inhibited in a Bub2 and Bfa1-dependent manner to prevent degradation of mitotic cyclins and anaphase entry. [16] Sic1 is a nice example demonstrating how systems-level feedbacks interact to sense the environmental conditions and trigger cell cycle transitions. Even though actual M-G1 transition is vastly complex with numerous proteins and regulations involved, dynamical systems approach allows simplification of this complex system into bistable input/output relation with two saddle-node bifurcations in which the output (mitotic exit) depends on critical concentration of Sic1. Using one-dimensional analysis, it might be possible to explain many of the irreversible transition points in the eukaryotic cell cycle that are governed by systems-level control and feedback. Other examples of irreversible transition points include Start (irreversible commitment to a new cell division cycle) that can be explained by irreversible bistable switch whose control parameter is tightly regulated by the systemic feedbacks involving Cln2, Whi5, and SBF. [17]

Reversibility of Mitotic Exit

Fig. 4 Flavopiridol induces reversible mitotic exit and cytokinesis if proteasome activity is inhibited. Figure 04.jpg
Fig. 4 Flavopiridol induces reversible mitotic exit and cytokinesis if proteasome activity is inhibited.

The regulation of mitotic progression and exit is of paramount importance, as errors in this process can lead to aneuploidy and cancer. Recent studies, notably involving the Cdk1 inhibitor flavopiridol, have shed light on the fascinating aspect of mitotic exit reversibility, challenging long-held views on the unidirectionality of cell cycle transitions. [18] Central to understanding mitotic exit is Cdk1, a kinase that orchestrates mitotic progression. Activated by binding to cyclins, particularly cyclin A and B, Cdk1's activity peaks during mitosis. [18] The timely degradation of these cyclins, especially cyclin B, is crucial for mitotic exit. This degradation ensures the dephosphorylation of Cdk1 substrates, acting as a timing mechanism for the critical transition from metaphase to anaphase, and eventually to the exit from mitosis. Such meticulous regulation highlights the cell's commitment to the irreversibility of the cell cycle phases under normal conditions. The introduction of flavopiridol, a potent Cdk1 inhibitor, has revolutionized the understanding of mitotic regulation. When applied to vertebrate cells during mitosis, flavopiridol induced a premature exit from mitosis, accompanied by cytokinesis, but notably without prior chromatid separation. [18] This phenomenon led to an intriguing 'cut' phenotype where chromosomes, although decondensed and enveloped in newly formed nuclear membranes, remained trapped within the cell's midbody (Figure 4). The most striking finding from the flavopiridol studies is the reversibility of mitotic exit. When the proteasome activity, responsible for cyclin degradation, is inhibited, flavopiridol's effects become reversible. [18] Upon the removal of flavopiridol, cells previously induced to exit mitosis prematurely could revert to a metaphase state. This reversal involves the reassembly of the mitotic spindle, retraction of the cytokinetic furrow, dissolution of the newly formed nuclear envelope, and recondensation of chromosomes.The reversibility of mitotic exit heavily relies on the stability of cyclin B. [18] In conditions where cyclin B is shielded from degradation, such as in the presence of proteasome inhibitors like MG132, the mitotic exit induced by flavopiridol can be reversed. [18] This finding was further corroborated using non-degradable cyclin B1, which showed that cells with stabilized cyclin B could revert to metaphase upon the removal of flavopiridol, indicating a direct correlation between cyclin B stability and mitotic exit reversibility. [18]

Mitotic Exit as a Cancer Therapeutic Target

Fig. 5 Knockdown of Cdc20 induces MOMP and non-MOMP death Figure 05.jpg
Fig. 5 Knockdown of Cdc20 induces MOMP and non-MOMP death

Cancer research has long focused on the disruption of cell division as a therapeutic strategy. Antimitotic drugs, targeting microtubule dynamics, have been a cornerstone of this approach. However, their success is often hampered by side effects like neurotoxicity and limited efficacy against solid tumors. [19] The use of taxanes and vinca alkaloids, targeting microtubule dynamics, has been a standard in cancer therapy. However, their efficacy is often undermined by severe side effects, notably neurotoxicity, due to the perturbation of microtubules in neurons. Additionally, spindle-specific antimitotic drugs, while less neurotoxic, have shown limited success against solid tumors, often comparable or inferior to traditional drugs. [19] This scenario underscores the need for alternative antimitotic strategies that are both more effective and less toxic. Recent studies have shifted focus to the process of mitotic exit, particularly blocking this phase as a novel anticancer strategy. The key player in this context is Cdc20, a regulatory protein that activates the Anaphase Promoting Complex/Cyclosome (APC/C), leading to cyclin B1 degradation and mitotic exit. Knockdown of Cdc20 using RNAi techniques induces a prolonged mitotic arrest, leading to cell death. [19] This approach has been shown to be more effective than spindle-perturbing drugs in killing cancer cells, including those resistant to apoptosis or prone to slippage (escape from drug-induced arrest). [19] Cdc20 knockdown leads to sustained mitotic arrest by stabilizing cyclin B1 levels, thereby preventing slippage. This prolonged arrest gives more time for apoptotic mechanisms to activate and kill the cancer cells. Unlike spindle-perturbing drugs, this strategy does not rely on spindle assembly checkpoint (SAC) activation and is effective even in SAC-deficient cells. [19] Moreover, Cdc20 knockdown induces cell death through both mitochondrial outer membrane permeabilization (MOMP)-dependent and -independent pathways, making it effective against a broader range of cancer cell types (Figure 5). [19] The blockade of mitotic exit presents several advantages over traditional spindle-targeting drugs. Firstly, it avoids the neurotoxicity associated with microtubule perturbation. Secondly, its efficacy is not limited by SAC activity or the cell's ability to evade death through slippage. Finally, it targets a fundamental and late stage of cell division, potentially reducing the chance of resistance development compared to drugs targeting earlier stages like spindle assembly. While promising, targeting mitotic exit as a cancer therapy is not without challenges. Developing specific, potent inhibitors of Cdc20 or other mitotic exit proteins is a significant hurdle. [19] Additionally, understanding the exact mechanisms by which prolonged mitotic arrest induces cell death, and identifying potential drug targets within these pathways, remains a key area of research.

Cell Polarity Proteins in Mitotic Exit

Fig. 6 Role of Ste20 in mitotic exit.(D) F-actin of wild-type and Dlte1 cells grown at 14 and 30degC was stained with rhodamine-phalloidin. (E) Serial dilutions of wild-type, Dlte1, Dste20 and Dlte1 Dste20 cells with Gal1-BUB2 were grown for 2 days at 30degC. (F) Dlte1 Dste20 cells have a ME defect. Wild-type, Dlte1, Dste20 and Dlte1 Dste20 cells with Gal1-BUB2 CDC14-GFP grown in YPRA medium were washed and incubated for 3 h at 30degC in YPRA galactose medium to induce Gal1-BUB2 expression. Cells were fixed, stained with DAPI and analysed by fluorescence microscopy. The circles in the cartoon cells indicate the DAPI staining regions. (G) An anaphase Gal1-BUB2 Dlte1 Dste20 CDC14-GFP cell of (F) Figure 06.jpg
Fig. 6 Role of Ste20 in mitotic exit.(D) F-actin of wild-type and Δlte1 cells grown at 14 and 30°C was stained with rhodamine–phalloidin. (E) Serial dilutions of wild-type, Δlte1, Δste20 and Δlte1 Δste20 cells with Gal1-BUB2 were grown for 2 days at 30°C. (F) Δlte1 Δste20 cells have a ME defect. Wild-type, Δlte1, Δste20 and Δlte1 Δste20 cells with Gal1-BUB2 CDC14-GFP grown in YPRA medium were washed and incubated for 3 h at 30°C in YPRA galactose medium to induce Gal1-BUB2 expression. Cells were fixed, stained with DAPI and analysed by fluorescence microscopy. The circles in the cartoon cells indicate the DAPI staining regions. (G) An anaphase Gal1-BUB2 Δlte1 Δste20 CDC14-GFP cell of (F)

In budding yeast, the process of mitotic exit involves a network of proteins that intertwine the concepts of cell polarity and mitotic regulation. Central to this network are the roles of Cdc14, a cell cycle phosphatase, and various cell polarity proteins, which collectively ensure the orderly transition from mitosis to the G1 phase of the cell cycle. [20] Cdc14 plays a pivotal role in mitotic exit by dephosphorylating key targets such as Cdh1/Hct1 and Sic1. [20] This action leads to a decrease in mitotic cyclin-dependent kinase (Cdk-Clb) activity, a prerequisite for mitotic exit. The regulation of Cdc14 is tightly controlled; it is sequestered in the nucleolus during interphase and metaphase and is released at the onset of anaphase. This release is crucial for Cdc14 to perform its function in triggering mitotic exit. [20] The release of Cdc14 from the nucleolus involves two essential steps: an initial partial release facilitated by separase (Esp1) and polo kinase (Cdc5) and a subsequent full release governed by the mitotic exit network (MEN). [20] The MEN is a GTPase-driven signaling network crucial for mitotic exit, and its proper functioning is essential for the cell cycle's fidelity. [20] A key player in the MEN is Tem1, a small Ras-like GTPase. The regulation of Tem1 is complex, involving its inactivation by the Bfa1-Bub2 GAP complex and activation by the GEF Lte1. [20] The spatial dynamics of these interactions are crucial; while Tem1 is associated with the spindle pole body (SPB), Lte1 is associated with the bud cortex but not the mother cell. This arrangement ensures that MEN activation and, consequently, mitotic exit occur only when the nucleus is correctly positioned within the bud. Cdc24, Cdc42, and Ste20 proteins, through their interaction with Lte1 and other MEN components, play a vital role in ensuring that mitotic exit is tightly regulated and occurs only under appropriate conditions (Figure 6). [20] For instance, the cell polarity protein Cdc42 regulates Ste20 activity, highlighting the interconnectedness of these pathways. [20]

Related Research Articles

<span class="mw-page-title-main">Cell division</span> Process by which living cells divide

Cell division is the process by which a parent cell divides into two daughter cells. Cell division usually occurs as part of a larger cell cycle in which the cell grows and replicates its chromosome(s) before dividing. In eukaryotes, there are two distinct types of cell division: a vegetative division (mitosis), producing daughter cells genetically identical to the parent cell, and a cell division that produces haploid gametes for sexual reproduction (meiosis), reducing the number of chromosomes from two of each type in the diploid parent cell to one of each type in the daughter cells. Mitosis is a part of the cell cycle, in which, replicated chromosomes are separated into two new nuclei. Cell division gives rise to genetically identical cells in which the total number of chromosomes is maintained. In general, mitosis is preceded by the S stage of interphase and is followed by telophase and cytokinesis; which divides the cytoplasm, organelles, and cell membrane of one cell into two new cells containing roughly equal shares of these cellular components. The different stages of mitosis all together define the M phase of an animal cell cycle—the division of the mother cell into two genetically identical daughter cells. To ensure proper progression through the cell cycle, DNA damage is detected and repaired at various checkpoints throughout the cycle. These checkpoints can halt progression through the cell cycle by inhibiting certain cyclin-CDK complexes. Meiosis undergoes two divisions resulting in four haploid daughter cells. Homologous chromosomes are separated in the first division of meiosis, such that each daughter cell has one copy of each chromosome. These chromosomes have already been replicated and have two sister chromatids which are then separated during the second division of meiosis. Both of these cell division cycles are used in the process of sexual reproduction at some point in their life cycle. Both are believed to be present in the last eukaryotic common ancestor.

<span class="mw-page-title-main">Anaphase</span> Stage of a cell division

Anaphase is the stage of mitosis after the process of metaphase, when replicated chromosomes are split and the newly-copied chromosomes are moved to opposite poles of the cell. Chromosomes also reach their overall maximum condensation in late anaphase, to help chromosome segregation and the re-formation of the nucleus.

<span class="mw-page-title-main">Telophase</span> Final stage of a cell division for eukaryotic cells both in mitosis and meiosis

Telophase is the final stage in both meiosis and mitosis in a eukaryotic cell. During telophase, the effects of prophase and prometaphase are reversed. As chromosomes reach the cell poles, a nuclear envelope is re-assembled around each set of chromatids, the nucleoli reappear, and chromosomes begin to decondense back into the expanded chromatin that is present during interphase. The mitotic spindle is disassembled and remaining spindle microtubules are depolymerized. Telophase accounts for approximately 2% of the cell cycle's duration.

<span class="mw-page-title-main">Anaphase-promoting complex</span> Cell-cycle regulatory complex

Anaphase-promoting complex is an E3 ubiquitin ligase that marks target cell cycle proteins for degradation by the 26S proteasome. The APC/C is a large complex of 11–13 subunit proteins, including a cullin (Apc2) and RING (Apc11) subunit much like SCF. Other parts of the APC/C have unknown functions but are highly conserved.

Maturation-promoting factor (abbreviated MPF, also called mitosis-promoting factor or M-Phase-promoting factor) is the cyclin–Cdk complex that was discovered first in frog eggs. It stimulates the mitotic and meiotic phases of the cell cycle. MPF promotes the entrance into mitosis (the M phase) from the G2 phase by phosphorylating multiple proteins needed during mitosis. MPF is activated at the end of G2 by a phosphatase, which removes an inhibitory phosphate group added earlier.

<span class="mw-page-title-main">Spindle checkpoint</span> Cell cycle checkpoint

The spindle checkpoint, also known as the metaphase-to-anaphase transition, the spindle assembly checkpoint (SAC), the metaphase checkpoint, or the mitotic checkpoint, is a cell cycle checkpoint during metaphase of mitosis or meiosis that prevents the separation of the duplicated chromosomes (anaphase) until each chromosome is properly attached to the spindle. To achieve proper segregation, the two kinetochores on the sister chromatids must be attached to opposite spindle poles. Only this pattern of attachment will ensure that each daughter cell receives one copy of the chromosome. The defining biochemical feature of this checkpoint is the stimulation of the anaphase-promoting complex by M-phase cyclin-CDK complexes, which in turn causes the proteolytic destruction of cyclins and proteins that hold the sister chromatids together.

G<sub>2</sub> phase Second growth phase in the eukaryotic cell cycle, prior to mitosis

G2 phase, Gap 2 phase, or Growth 2 phase, is the third subphase of interphase in the cell cycle directly preceding mitosis. It follows the successful completion of S phase, during which the cell’s DNA is replicated. G2 phase ends with the onset of prophase, the first phase of mitosis in which the cell’s chromatin condenses into chromosomes.

A spindle poison, also known as a spindle toxin, is a poison that disrupts cell division by affecting the protein threads that connect the centromere regions of chromosomes, known as spindles. Spindle poisons effectively cease the production of new cells by interrupting the mitosis phase of cell division at the spindle assembly checkpoint (SAC). However, as numerous and varied as they are, spindle poisons are not yet 100% effective at ending the formation of tumors (neoplasms). Although not 100% effective, substantive therapeutic efficacy has been found in these types of chemotherapeutic treatments. The mitotic spindle is composed of microtubules that aid, along with regulatory proteins, each other in the activity of appropriately segregating replicated chromosomes. Certain compounds affecting the mitotic spindle have proven highly effective against solid tumors and hematological malignancies.

<span class="mw-page-title-main">Separase</span> Mammalian protein found in Homo sapiens

Separase, also known as separin, is a cysteine protease responsible for triggering anaphase by hydrolysing cohesin, which is the protein responsible for binding sister chromatids during the early stage of anaphase. In humans, separin is encoded by the ESPL1 gene.

Securin is a protein involved in control of the metaphase-anaphase transition and anaphase onset. Following bi-orientation of chromosome pairs and inactivation of the spindle checkpoint system, the underlying regulatory system, which includes securin, produces an abrupt stimulus that induces highly synchronous chromosome separation in anaphase.

<span class="mw-page-title-main">Cell cycle checkpoint</span> Control mechanism in the eukaryotic cell cycle

Cell cycle checkpoints are control mechanisms in the eukaryotic cell cycle which ensure its proper progression. Each checkpoint serves as a potential termination point along the cell cycle, during which the conditions of the cell are assessed, with progression through the various phases of the cell cycle occurring only when favorable conditions are met. There are many checkpoints in the cell cycle, but the three major ones are: the G1 checkpoint, also known as the Start or restriction checkpoint or Major Checkpoint; the G2/M checkpoint; and the metaphase-to-anaphase transition, also known as the spindle checkpoint. Progression through these checkpoints is largely determined by the activation of cyclin-dependent kinases by regulatory protein subunits called cyclins, different forms of which are produced at each stage of the cell cycle to control the specific events that occur therein.

Polo-like kinases (Plks) are regulatory serine/threonine kinases of the cell cycle involved in mitotic entry, mitotic exit, spindle formation, cytokinesis, and meiosis. Only one Plk is found in the genomes of the fly Drosophila melanogaster (Polo), budding yeast (Cdc5) and fission yeast (Plo1). Vertebrates and other animals, however, have many Plk family members including Plk1, Plk2/Snk, Plk3/Prk/FnK, Plk4/Sak and Plk5. Of the vertebrate Plk family members, the mammalian Plk1 has been most extensively studied. During mitosis and cytokinesis, Plks associate with several structures including the centrosome, kinetochores, and the central spindle.

<span class="mw-page-title-main">CDC20</span> Protein-coding gene in the species Homo sapiens

The cell division cycle protein 20 homolog is an essential regulator of cell division that is encoded by the CDC20 gene in humans. To the best of current knowledge its most important function is to activate the anaphase promoting complex (APC/C), a large 11-13 subunit complex that initiates chromatid separation and entrance into anaphase. The APC/CCdc20 protein complex has two main downstream targets. Firstly, it targets securin for destruction, enabling the eventual destruction of cohesin and thus sister chromatid separation. It also targets S and M-phase (S/M) cyclins for destruction, which inactivates S/M cyclin-dependent kinases (Cdks) and allows the cell to exit from mitosis. A closely related protein, Cdc20homologue-1 (Cdh1) plays a complementary role in the cell cycle.

Sic1, a protein, is a stoichiometric inhibitor of Cdk1-Clb complexes in the budding yeast Saccharomyces cerevisiae. Because B-type cyclin-Cdk1 complexes are the drivers of S-phase initiation, Sic1 prevents premature S-phase entry. Multisite phosphorylation of Sic1 is thought to time Sic1 ubiquitination and destruction, and by extension, the timing of S-phase entry.

A series of biochemical switches control transitions between and within the various phases of the cell cycle. The cell cycle is a series of complex, ordered, sequential events that control how a single cell divides into two cells, and involves several different phases. The phases include the G1 and G2 phases, DNA replication or S phase, and the actual process of cell division, mitosis or M phase. During the M phase, the chromosomes separate and cytokinesis occurs.

Cdc14 and Cdc14 are a gene and its protein product respectively. Cdc14 is found in most of the eukaryotes. Cdc14 was defined by Hartwell in his famous screen for loci that control the cell cycle of Saccharomyces cerevisiae. Cdc14 was later shown to encode a protein phosphatase. Cdc14 is dual-specificity, which means it has serine/threonine and tyrosine-directed activity. A preference for serines next to proline is reported. Many early studies, especially in the budding yeast Saccharomyces cerevisiae, demonstrated that the protein plays a key role in regulating late mitotic processes. However, more recent work in a range of systems suggests that its cellular function is more complex.

<span class="mw-page-title-main">Mad1</span>

Mad1 is a non-essential protein which in yeast has a function in the spindle assembly checkpoint (SAC). This checkpoint monitors chromosome attachment to spindle microtubules and prevents cells from starting anaphase until the spindle is built up. The name Mad refers to the observation that mutant cells are mitotic arrest deficient (MAD) during microtubule depolymerization. Mad1 recruits the anaphase inhibitor Mad2 to unattached kinetochores and is essential for Mad2-Cdc20 complex formation in vivo but not in vitro. In vivo, Mad1 acts as a competitive inhibitor of the Mad2-Cdc20 complex. Mad1 is phosphorylated by Mps1 which then leads together with other activities to the formation of the mitotic checkpoint complex (MCC). Thereby it inhibits the activity of the anaphase-promoting complex/cyclosome (APC/C). Homologues of Mad1 are conserved in eukaryotes from yeast to mammals.

<span class="mw-page-title-main">APC/C activator protein CDH1</span> Fungal protein found in Saccharomyces cerevisiae S288c

Cdh1 is one of the substrate adaptor proteins of the anaphase-promoting complex (APC) in the budding yeast Saccharomyces cerevisiae. Functioning as an activator of the APC/C, Cdh1 regulates the activity and substrate specificity of this ubiquitin E3-ligase. The human homolog is encoded by the FZR1 gene, which is not to be confused with the CDH1 gene.

Clb5 and Clb6 are B-type, S-phase cyclins in yeast that assist in cell cycle regulation. Clb5 and Clb6 bind and activate Cdk1, and high levels of these cyclins are required for entering S-phase. S-phase cyclin binding to Cdk1 directly stimulates DNA replication as well as progression to the next phase of the cell cycle.

<span class="mw-page-title-main">Motifs targeted by APC/C</span>

The anaphase- promoting complex or cyclosome (APC/C) is a highly specific ubiquitin protein ligase responsible for triggering events of late mitosis. In early mitosis, Cdc20 levels rise and APC/C binds to form active APC/CCdc20. This then leads to the destruction of mitotic cyclins, securin, and other proteins to trigger chromosome separation in anaphase. In early anaphase, Cdk1 is inactivated, leading to the activation of Cdh1, the other activator subunit of APC/C. This then triggers the degradation of Cdc20 and leads to the activation of APC/CCdh1 through G1 to suppress S- phase cyclin-Cdk activity. At the end of G1, APC/CCdh1 is inactivated and S- phase and mitotic cyclins gets reaccumulate as the cell progresses to S phase.

References

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