Nickel-dependent hydrogenase

Nickel-dependent hydrogenase
Identifiers
Symbol	NiFeSe_Hases
Pfam	PF00374
InterPro	IPR001501
PROSITE	PDOC00400
SCOP2	1frv / SCOPe / SUPFAM
TCDB	3.D.7
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary PDB 1e3d D:50-543 1h2r L:59-552 1ubj L:59-552 1ubl L:59-552 1wul L:59-552 1h2a L:59-552 1ubt L:59-552 1wuj L:59-552 1ubu L:59-552 1ubk L:59-552 1ubh L:59-552 1ubm L:59-552 1wuk L:59-552 1wuh L:59-552 1wui L:59-552 1ubo L:59-552 1ubr L:59-552 1frf L:50-549 1yqw Q:50-548 1yq9 H:43-535 1cc1 L:49-498

Hydrogenases are enzymes that catalyze the reversible activation of hydrogen and which occur widely in prokaryotes as well as in some eukaryotes. There are various types of hydrogenases, but all of them seem to contain at least one iron-sulphur cluster. They can be broadly divided into two groups: hydrogenases containing nickel and, in some cases, also selenium (the [NiFe] and [NiFeSe] hydrogenases) and those lacking nickel (the [Fe] hydrogenases).

The [NiFe] and [NiFeSe] hydrogenases are heterodimer that consist of a small subunit that contains a signal peptide and a large subunit. All the known large subunits seem to be evolutionary related; ^[1] they contain two Cys-x-x-Cys motifs; one at their N-terminal end; the other at their C-terminal end. These four cysteines are involved in the binding of nickel. ^[2] In the [NiFeSe] hydrogenases the first cysteine of the C-terminal motif is a selenocysteine which has experimentally been shown to be a nickel ligand. ^[3]

References

1. Przybyla AE, Robbins J, Menon NK, Chatelus CY, Peck HD, Choi ES (1990). "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames". J. Bacteriol. 172 (4): 1969–1977. doi:10.1128/jb.172.4.1969-1977.1990. PMC   208693 . PMID   2180913.
2. Volbeda A, Hatchikian EC, Piras C, Frey M, Fontecilla-Camps JC, Charon MH (1995). "Crystal structure of the nickel-iron hydrogenase from Desulfovibrio gigas". Nature. 373 (6515): 580–587. Bibcode:1995Natur.373..580V. doi:10.1038/373580a0. PMID   7854413. S2CID   4335445.
3. Moura I, Eidsness MK, Scott RA, Moura JJ, Legall J, Peck Jr HD, Prickril BC, DerVartanian DV (1989). "Evidence for selenocysteine coordination to the active site nickel in the [NiFeSe]hydrogenases from Desulfovibrio baculatus". Proc. Natl. Acad. Sci. U.S.A. 86 (1): 147–151. Bibcode:1989PNAS...86..147E. doi: 10.1073/pnas.86.1.147 . PMC   286421 . PMID   2521386.

This article incorporates text from the public domain Pfam and InterPro: IPR001501