Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye. There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy.
Optics is the branch of physics that studies the behaviour and properties of light, including its interactions with matter and the construction of instruments that use or detect it. Optics usually describes the behaviour of visible, ultraviolet, and infrared light. Because light is an electromagnetic wave, other forms of electromagnetic radiation such as X-rays, microwaves, and radio waves exhibit similar properties.
In optics, an aperture is a hole or an opening through which light travels. More specifically, the aperture and focal length of an optical system determine the cone angle of the bundle of rays that come to a focus in the image plane.
A pinhole camera is a simple camera without a lens but with a tiny aperture —effectively a light-proof box with a small hole in one side. Light from a scene passes through the aperture and projects an inverted image on the opposite side of the box, which is known as the camera obscura effect. The size of the images depends on the distance between the object and the pinhole.
A camera lens is an optical lens or assembly of lenses used in conjunction with a camera body and mechanism to make images of objects either on photographic film or on other media capable of storing an image chemically or electronically.
Optics is the branch of physics which involves the behavior and properties of light, including its interactions with matter and the construction of instruments that use or detect it. Optics usually describes the behavior of visible, ultraviolet, and infrared light. Because light is an electromagnetic wave, other forms of electromagnetic radiation such as X-rays, microwaves, and radio waves exhibit similar properties.
In optics, any optical instrument or system – a microscope, telescope, or camera – has a principal limit to its resolution due to the physics of diffraction. An optical instrument is said to be diffraction-limited if it has reached this limit of resolution performance. Other factors may affect an optical system's performance, such as lens imperfections or aberrations, but these are caused by errors in the manufacture or calculation of a lens, whereas the diffraction limit is the maximum resolution possible for a theoretically perfect, or ideal, optical system.
An optical cavity, resonating cavity or optical resonator is an arrangement of mirrors or other optical elements that forms a cavity resonator for light waves. Optical cavities are a major component of lasers, surrounding the gain medium and providing feedback of the laser light. They are also used in optical parametric oscillators and some interferometers. Light confined in the cavity reflects multiple times, producing modes with certain resonance frequencies. Modes can be decomposed into longitudinal modes that differ only in frequency and transverse modes that have different intensity patterns across the cross-section of the beam. Many types of optical cavity produce standing wave modes.
A zone plate is a device used to focus light or other things exhibiting wave character. Unlike lenses or curved mirrors, zone plates use diffraction instead of refraction or reflection. Based on analysis by French physicist Augustin-Jean Fresnel, they are sometimes called Fresnel zone plates in his honor. The zone plate's focusing ability is an extension of the Arago spot phenomenon caused by diffraction from an opaque disc.
A collimator is a device which narrows a beam of particles or waves. To narrow can mean either to cause the directions of motion to become more aligned in a specific direction, or to cause the spatial cross section of the beam to become smaller.
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Capturing multiple two-dimensional images at different depths in a sample enables the reconstruction of three-dimensional structures within an object. This technique is used extensively in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.
A dichroic filter, thin-film filter, or interference filter is a color filter used to selectively pass light of a small range of colors while reflecting other colors. By comparison, dichroic mirrors and dichroic reflectors tend to be characterized by the colors of light that they reflect, rather than the colors they pass.
Cone tracing and beam tracing are a derivative of the ray tracing algorithm that replaces rays, which have no thickness, with thick rays.
In signal processing, apodization is the modification of the shape of a mathematical function. The function may represent an electrical signal, an optical transmission, or a mechanical structure. In optics, it is primarily used to remove Airy disks caused by diffraction around an intensity peak, improving the focus.
A spatial filter is an optical device which uses the principles of Fourier optics to alter the structure of a beam of light or other electromagnetic radiation, typically coherent laser light. Spatial filtering is commonly used to "clean up" the output of lasers, removing aberrations in the beam due to imperfect, dirty, or damaged optics, or due to variations in the laser gain medium itself. This filtering can be applied to transmit a pure transverse mode from a multimode laser while blocking other modes emitted from the optical resonator. The term "filtering" indicates that the desirable structural features of the original source pass through the filter, while the undesirable features are blocked. An apparatus which follows the filter effectively sees a higher-quality but lower-powered image of the source, instead of the actual source directly. An example of the use of spatial filter can be seen in advanced setup of micro-Raman spectroscopy.
Argus was a two-beam high power infrared neodymium doped silica glass laser with a 20 cm (7.9 in) output aperture built at Lawrence Livermore National Laboratory in 1976 for the study of inertial confinement fusion. Argus advanced the study of laser-target interaction and paved the way for the construction of its successor, the 20 beam Shiva laser.
Cyclops was a high-power laser built at the Lawrence Livermore National Laboratory (LLNL) in 1975. It was the second laser constructed in the lab's Laser program, which aimed to study inertial confinement fusion (ICF).
A point diffraction interferometer (PDI) is a type of common-path interferometer. Unlike an amplitude-splitting interferometer, such as a Michelson interferometer, which separates out an unaberrated beam and interferes this with the test beam, a common-path interferometer generates its own reference beam. In PDI systems, the test and reference beams travel the same or almost the same path. This design makes the PDI extremely useful when environmental isolation is not possible or a reduction in the number of precision optics is required. The reference beam is created from a portion of the test beam by diffraction from a small pinhole in a semitransparent coating. The principle of a PDI is shown in Figure 1.
A laser beam profiler captures, displays, and records the spatial intensity profile of a laser beam at a particular plane transverse to the beam propagation path. Since there are many types of lasers — ultraviolet, visible, infrared, continuous wave, pulsed, high-power, low-power — there is an assortment of instrumentation for measuring laser beam profiles. No single laser beam profiler can handle every power level, pulse duration, repetition rate, wavelength, and beam size.
The Raman microscope is a laser-based microscopic device used to perform Raman spectroscopy. The term MOLE is used to refer to the Raman-based microprobe. The technique used is named after C. V. Raman, who discovered the scattering properties in liquids.
