A pronucleus (PL: pronuclei) denotes the nucleus found in either a sperm or egg cell during the process of fertilization. The sperm cell undergoes a transformation into a pronucleus after entering the egg cell but prior to the fusion of the genetic material of both the sperm and egg. In contrast, the egg cell possesses a pronucleus once it becomes haploid, not upon the arrival of the sperm cell. Haploid cells, such as sperm and egg cells in humans, carry half the number of chromosomes present in somatic cells, with 23 chromosomes compared to the 46 found in somatic cells. It is noteworthy that the male and female pronuclei do not physically merge, although their genetic material does. Instead, their membranes dissolve, eliminating any barriers between the male and female chromosomes, facilitating the combination of their chromosomes into a single diploid nucleus in the resulting embryo, which contains a complete set of 46 chromosomes.
The presence of two pronuclei serves as the initial indication of successful fertilization, often observed around 18 hours after insemination, or intracytoplasmic sperm injection (ICSI) during in vitro fertilization. At this stage, the zygote is termed a two-pronuclear zygote (2PN). Two-pronuclear zygotes transitioning through 1PN or 3PN states tend to yield poorer-quality embryos compared to those maintaining 2PN status throughout development, [1] and this distinction may hold significance in the selection of embryos during in vitro fertilization (IVF) procedures.
The pronucleus was discovered the 1870s microscopically using staining techniques combined with microscopes with improved magnification levels. The pronucleus was originally found during the first studies on meiosis. Edouard Van Beneden published a paper in 1875 in which he first mentions the pronucleus by studying the eggs of rabbits and bats. He stated that the two pronuclei form together in the center of the cell to form the embryonic nucleus. Van Beneden also found that the sperm enters into the cell through the membrane in order to form the male pronucleus. In 1876, Oscar Hertwig did a study on sea urchin eggs because the eggs of sea urchins are transparent, so it allowed for much better magnification of the egg. Hertwig confirmed Van Beneden's finding of the pronucleus, and also found the formation of the female pronucleus involves the formation of polar bodies. [2]
The female pronucleus is the female egg cell once it has become a haploid cell, and the male pronucleus forms when the sperm enters into the female egg. While the sperm develops inside of the male testes, the sperm does not become a pronucleus until it decondenses quickly inside of the female egg. [3] When the sperm reaches the female egg, the sperm loses its outside membrane as well as its tail. The sperm does this because the membrane and the tail are no longer needed by the female ovum. The purpose of the cell membrane was to protect the DNA from the acidic vaginal fluid, and the purpose of the tail of the sperm was to help move the sperm cell to the egg cell. The formation of the female egg is asymmetrical, while the formation of the male sperm is symmetrical. Typically in a female mammal, meiosis starts with one diploid cell and becomes one haploid ovum and typically two polar bodies, however one may later divide to form a third polar body. [4] In a male, meiosis starts with one diploid cell and ends with four sperm. [5] In mammals, the female pronucleus starts in the center of the egg before fertilization. When the male pronucleus is formed, after the sperm cell reaches the egg, the two pronuclei migrate towards each other. However, in brown alga Pelvetia , the egg pronucleus starts in the center of the egg before fertilization and remain in the center after fertilization. This is because the egg cells of brown alga Pelvetia, the egg pronucleus is anchored down by microtubules so only the male pronucleus migrates towards the female pronucleus. [6]
The calcium concentration within the egg cell cytoplasm has a very important role in the formation of an activated female egg. If there is no calcium influx, the female diploid cell will produce three pronuclei, rather than only one. This is due to the failure of release of the second polar body. [7]
In sea urchins, the formation of the zygote starts with the fusion of both the inner and outer nuclei of the male and female pronuclei. It is unknown if one of the pronuclei start the combination of the two, or if the microtubules that help the dissolution of membranes commence the action. [8] The microtubules that make the two pronuclei combine come from the sperm's centrosome. There is a study that strongly supports that microtubules are an important part of the fusion of the pronuclei. Vinblastine is a chemotherapy drug that affects both the plus and minus ends of microtubules. [9] When vinblastine is added to the ovum, there is a high rate of pronuclear fusion failure. This high rate of pronuclear fusion failure highly suggests that microtubules play a major role in the fusion of the pronucleus. [10] In mammals, the pronuclei only last in the cell for about twelve hours, due to the fusion of the genetic material of the two pronuclei within the egg cell. Many studies of pronuclei have been in the egg cells of sea urchins, where the pronuclei are in the egg cell for less than an hour. The main difference between the process of fusion of genetic materials in mammals versus sea urchins is that in sea urchins, the pronuclei go directly into forming a zygote nucleus. In mammalian egg cells, the chromatin from the pronuclei form chromosomes that merge onto the same mitotic spindle. The diploid nucleus in mammals is first seen at the two-cell stage, whereas in sea urchins it is first found at the zygote stage. [3]
A gamete is a haploid cell that fuses with another haploid cell during fertilization in organisms that reproduce sexually. Gametes are an organism's reproductive cells, also referred to as sex cells. In species that produce two morphologically distinct types of gametes, and in which each individual produces only one type, a female is any individual that produces the larger type of gamete—called an ovum— and a male produces the smaller type—called a sperm. Sperm cells or spermatozoa are small and motile due to the flagellum, a tail-shaped structure that allows the cell to propel and move. In contrast, each egg cell or ovum is relatively large and non-motile. In short a gamete is an egg cell or a sperm. In animals, ova mature in the ovaries of females and sperm develop in the testes of males. During fertilization, a spermatozoon and ovum unite to form a new diploid organism. Gametes carry half the genetic information of an individual, one ploidy of each type, and are created through meiosis, in which a germ cell undergoes two fissions, resulting in the production of four gametes. In biology, the type of gamete an organism produces determines the classification of its sex.
A gametophyte is one of the two alternating multicellular phases in the life cycles of plants and algae. It is a haploid multicellular organism that develops from a haploid spore that has one set of chromosomes. The gametophyte is the sexual phase in the life cycle of plants and algae. It develops sex organs that produce gametes, haploid sex cells that participate in fertilization to form a diploid zygote which has a double set of chromosomes. Cell division of the zygote results in a new diploid multicellular organism, the second stage in the life cycle known as the sporophyte. The sporophyte can produce haploid spores by meiosis that on germination produce a new generation of gametophytes.
Meiosis is a special type of cell division of germ cells in sexually-reproducing organisms that produces the gametes, such as sperm or egg cells. It involves two rounds of division that ultimately result in four cells with only one copy of each chromosome (haploid). Additionally, prior to the division, genetic material from the paternal and maternal copies of each chromosome is crossed over, creating new combinations of code on each chromosome. Later on, during fertilisation, the haploid cells produced by meiosis from a male and a female will fuse to create a cell with two copies of each chromosome again, the zygote.
Sex is the trait that determines whether a sexually reproducing organism produces male or female gametes. Male organisms produce small mobile gametes, while female organisms produce larger, non-mobile gametes. Organisms that produce both types of gametes are called hermaphrodites. During sexual reproduction, male and female gametes fuse to form zygotes, which develop into offspring that inherit traits from each parent.
A spermatozoon is a motile sperm cell, or moving form of the haploid cell that is the male gamete. A spermatozoon joins an ovum to form a zygote.
A zygote is a eukaryotic cell formed by a fertilization event between two gametes. The zygote's genome is a combination of the DNA in each gamete, and contains all of the genetic information of a new individual organism.
Fertilisation or fertilization, also known as generative fertilisation, syngamy and impregnation, is the fusion of gametes to give rise to a new individual organism or offspring and initiate its development. While processes such as insemination or pollination which happen before the fusion of gametes are also sometimes informally referred to as fertilisation, these are technically separate processes. The cycle of fertilisation and development of new individuals is called sexual reproduction. During double fertilisation in angiosperms the haploid male gamete combines with two haploid polar nuclei to form a triploid primary endosperm nucleus by the process of vegetative fertilisation.
Alternation of generations is the predominant type of life cycle in plants and algae. In plants both phases are multicellular: the haploid sexual phase – the gametophyte – alternates with a diploid asexual phase – the sporophyte.
The egg cell, or ovum, is the female reproductive cell, or gamete, in most anisogamous organisms. The term is used when the female gamete is not capable of movement (non-motile). If the male gamete (sperm) is capable of movement, the type of sexual reproduction is also classified as oogamous. A nonmotile female gamete formed in the oogonium of some algae, fungi, oomycetes, or bryophytes is an oosphere. When fertilized the oosphere becomes the oospore.
A sporophyte is the diploid multicellular stage in the life cycle of a plant or alga which produces asexual spores. This stage alternates with a multicellular haploid gametophyte phase.
During fertilization, a sperm must first fuse with the plasma membrane and then penetrate the female egg cell to fertilize it. Fusing to the egg cell usually causes little problem, whereas penetrating through the egg's hard shell or extracellular matrix can be more difficult. Therefore, sperm cells go through a process known as the acrosome reaction, which is the reaction that occurs in the acrosome of the sperm as it approaches the egg.
In seed plants, the ovule is the structure that gives rise to and contains the female reproductive cells. It consists of three parts: the integument, forming its outer layer, the nucellus, and the female gametophyte in its center. The female gametophyte — specifically termed a megagametophyte— is also called the embryo sac in angiosperms. The megagametophyte produces an egg cell for the purpose of fertilization. The ovule is a small structure present in the ovary. It is attached to the placenta by a stalk called a funicle. The funicle provides nourishment to the ovule.
Karyogamy is the final step in the process of fusing together two haploid eukaryotic cells, and refers specifically to the fusion of the two nuclei. Before karyogamy, each haploid cell has one complete copy of the organism's genome. In order for karyogamy to occur, the cell membrane and cytoplasm of each cell must fuse with the other in a process known as plasmogamy. Once within the joined cell membrane, the nuclei are referred to as pronuclei. Once the cell membranes, cytoplasm, and pronuclei fuse, the resulting single cell is diploid, containing two copies of the genome. This diploid cell, called a zygote or zygospore can then enter meiosis, or continue to divide by mitosis. Mammalian fertilization uses a comparable process to combine haploid sperm and egg cells (gametes) to create a diploid fertilized egg.
Human fertilization is the union of a human egg and sperm, occurring primarily in the ampulla of the fallopian tube. The result of this union leads to the production of a fertilized egg called a zygote, initiating embryonic development. Scientists discovered the dynamics of human fertilization in the nineteenth century.
In biology, polyspermy describes the fertilization of an egg by more than one sperm. Diploid organisms normally contain two copies of each chromosome, one from each parent. The cell resulting from polyspermy, on the other hand, contains three or more copies of each chromosome—one from the egg and one each from multiple sperm. Usually, the result is an unviable zygote. This may occur because sperm are too efficient at reaching and fertilizing eggs due to the selective pressures of sperm competition. Such a situation is often deleterious to the female: in other words, the male–male competition among sperm spills over to create sexual conflict.
A polar body is a small haploid cell that is formed at the same time as an egg cell during oogenesis, but generally does not have the ability to be fertilized. It is named from its polar position in the egg.
Double fertilization is a complex fertilization mechanism of flowering plants (angiosperms). This process involves the joining of a female gametophyte with two male gametes (sperm). It begins when a pollen grain adheres to the stigma of the carpel, the female reproductive structure of a flower. The pollen grain then takes in moisture and begins to germinate, forming a pollen tube that extends down toward the ovary through the style. The tip of the pollen tube then enters the ovary and penetrates through the micropyle opening in the ovule. The pollen tube proceeds to release the two sperm in the embryo sac.
Sexual reproduction is a type of reproduction that involves a complex life cycle in which a gamete with a single set of chromosomes combines with another gamete to produce a zygote that develops into an organism composed of cells with two sets of chromosomes (diploid). This is typical in animals, though the number of chromosome sets and how that number changes in sexual reproduction varies, especially among plants, fungi, and other eukaryotes.
Oocyteactivation is a series of processes that occur in the oocyte during fertilization.
Androgenesis occurs when a zygote is produced with only paternal nuclear genes. During standard sexual reproduction, one female and one male parent each produce haploid gametes, which recombine to create offspring with genetic material from both parents. However, in androgenesis, there is no recombination of maternal and paternal chromosomes, and only the paternal chromosomes are passed down to the offspring. The offspring produced in androgenesis will still have maternally inherited mitochondria, as is the case with most sexually reproducing species.