SRG1 RNA

Last updated
SRG1
Srg1.png
Conserved secondary structure of SRG1 RNA
Identifiers
SymbolSRG1
Rfam RF01765
Other data
RNA type Gene; Cis-reg;
Domain(s) Saccharomyces
PDB structures PDBe

SRG1 RNA (SER3 regulatory gene 1) is a non-coding RNA which represses the expression of SER3 (YER081W). [1] [2] [3] SER3 is a gene which codes for a phosphoglycerate dehydrogenase involved in the biosynthesis of serine. [4] SRG1 represses SER3 expression via transcription interference, and in that respect is the first intergenic transcript of its kind. [1]

Contents

SRG1 was identified when chromatin immunoprecipitation (ChIP) assays showed that in Saccharomyces cerevisiae , even when SER3 was being repressed, TATA-binding protein and RNA Polymerase II were still bound to the SER3 DNA in such a way that should have caused active transcription. [1] Further analysis found a second TATA box 558 bp upstream of SER3. A bioinformatic scan identified this same TATA box element in related Saccharomyces. [5] SRG1 has been found to be cis-acting, it had no repressive effect when in trans. [1]

SRG1 RNA is unrelated to Senescence Related Gene 1 (SRG1), a protein-coding gene found in Arabidopsis thaliana , [6] and is also distinct from Stress Response Gene 1 (SRG1) found in Medicago sativa . [7] [8]

References

  1. 1 2 3 4 Martens JA, Laprade L, Winston F (June 2004). "Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene". Nature. 429 (6991): 571–574. Bibcode:2004Natur.429..571M. doi:10.1038/nature02538. PMID   15175754. S2CID   809550.
  2. ↑ "Entrez Gene SRG1". 2010-07-03. Retrieved 2010-08-02.
  3. ↑ "Yeast Genome SRG1" . Retrieved 2010-08-02.
  4. ↑ Albers E, Laizé V, Blomberg A, Hohmann S, Gustafsson L (March 2003). "Ser3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiae". The Journal of Biological Chemistry. 278 (12): 10264–10272. doi: 10.1074/jbc.M211692200 . PMID   12525494.
  5. ↑ Cliften P, Sudarsanam P, Desikan A, Fulton L, Fulton B, Majors J, Waterston R, Cohen BA, Johnston M (July 2003). "Finding functional features in Saccharomyces genomes by phylogenetic footprinting". Science. 301 (5629): 71–76. Bibcode:2003Sci...301...71C. doi: 10.1126/science.1084337 . PMID   12775844. S2CID   1305166.
  6. ↑ "Uniprot Knowledgebase - Protein SRG1" . Retrieved 2010-08-02.
  7. ↑ Truesdell GM, Dickman MB (March 1997). "Isolation of pathogen/stress-inducible cDNAs from alfalfa by mRNA differential display" (PDF). Plant Molecular Biology. 33 (4): 737–743. doi:10.1023/A:1005728420374. PMID   9132065. S2CID   35519690.
  8. ↑ "GenBank Accs:U42752.1". 27 May 1997. Retrieved 2010-08-02.

Further reading

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