Social motility

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Social motility of African trypanosomiasis African Trypanosome (8093712590).jpg
Social motility of African trypanosomiasis

Social motility describes the motile movement of groups of cells that communicate with each other to coordinate movement based on external stimuli. There are multiple varieties of each kingdom that express social motility that provides a unique evolutionary advantages that other species do not possess. This has made them lethal killers such as African trypanosomiasis , [1] or Myxobacteria . [2] These evolutionary advantages have proven to increase survival rate among socially motile bacteria whether it be the ability to evade predators [3] or communication within a swarm to form spores for long term hibernation in times of low nutrients or toxic environments. [4]

Contents

Measuring bacterial motility

Motility assays can be utilized to quantitatively measure the macroscopic motility of a specimen. To perform a motility assay, semi-solid agar is inoculated with a small amount of a liquid suspension containing the specimen of interest. Over time, bacteria that are non-motile will remain near the initial inoculation site, while motile bacteria will spread along the media, forming a visible blur. The radius of the area of motility can be measured and compared between specimens, while the spatial patterns and spread of the visible area of motility can be altered by adding low concentrations of a known chemoattractant or chemorepellent to the medium. [5] The motility of a species can also be measured microscopically, giving more insight into the movement of individual cells. Colonies can be examined under a microscope by using a thin layer of solidified nutrient media and a glass coverslip to create an interstitial interface at which active colony expansion can occur [6] . This allows for the visualization of individual cells and the identification of different forms of bacterial motility present in a colony.

Communication

Bacterial cells are able to communicate with one another through the use of chemical messengers. These chemical messengers are passed from one cell to the next to control factors such as virulence, growth and nutrient conditions, etc. As first discovered in plants, diffusible signal factors (DSFs) have been found in bacteria such as Burkholderia cenocepacia and Pseudomonas aeruginosa. [7] When individual cells are stimulated by DSF, it causes them to release their own DSF to spread the signal further and also to generate a response to the DSF often seen as growth, movement, or sporulation in unfavorable growth conditions. Via these chemical messengers, swarms of bacteria are able to increase the rate of survival compared to single cell bacteria on their own.

Benefits

Predation

Traveling in groups, often referred to as swarms, is beneficial to the organism. For instance, when Myxobacteria swarms and feeds on prey, all individual cells release hydrolytic enzymes. This abundance of metabolic enzymes allows the swarm to easily degrade and engulf the prey. [2] Interactions between separate species of organisms in a given environment is very common. Production of toxins, usually in the form of antibodies, allows for cells to ward off other organisms from infringing on their niche. Similar to the combined release of degrading enzymes, antibodies allow for a colony of bacteria to fight off surrounding organisms in the same habitat.

Survival

Increased survival rates are seen in motile bacteria. This can be attributed to factors such as Chemotaxis, bacteria's ability to sense and migrate towards nutrients. The Chemotaxis mechanism can be amplified by social motility to alert all cells in the cluster of bacteria to move towards nutrients. The same is true of any toxic substances and the avoidance of that toxic environment by motile bacteria.

Phototaxis is a similar intracellular process to chemotaxis, and involves the directed movement of organisms in response to light. Prokaryotes are unable to sense the exact direction of light, but have still evolved mechanisms to sense and respond to the light-intensity gradient. Some halophilic archaebacteria, such as Halobacterium salinarum , use sensory rhodopsins as receptors for light and can help direct bacterial swims in areas with steep light gradients [8] . This process is also present in eukaryotic organisms such as the green algae Chlamydomonas reinhardtii which using phototaxis to drive movement towards light to increase photosynthesis or away from areas of bright light to avoid damage to the molecular processes involved in photosynthesis [9] .

Reproduction

Some organisms use social motility as a way to reproduce. One such organism is the slime mold Dictostelium discoideum , which forms a mobile “slug” via the aggregation of many individual amoebas. This process begins by one amoeba releasing a cyclic AMP (cAMP) signal during periods of stress, resulting in neighboring amoebas moving to this higher cAMP concentration through chemotaxis and releasing their own cAMP signals. The amoebas eventually aggregate into a single “slug,” which responds to moisture and light gradients as it searches for a good place to form a reproductive stalk and produce spores [10] .

Examples

Swarming motility of Pseudomonas aeruginosa. Swarming motility of Pseudomonas aeruginosa.jpg
Swarming motility of Pseudomonas aeruginosa .

Swarming

Swarming motility is the coordinated movement of bacteria along a solid/semisolid surface. Swarming motility can usually be observed in a laboratory setting, depending on the conditions of media nutrient concentration, and the viscosity of the surface of the media. More information on swarming motility can be found here. [11]

Gliding

Example of Social Motility of Myxococcus xanthus.

Mechanisms that drive gliding motility are still unknown. However, despite lacking flagella, pili, and fimbriae, bacteria such as Myxococcus xanthus are able to move across surfaces in a gliding motion. Close studies of the myxococcus xanthus has proposed ideas of how the bacteria are able to move across surfaces. [12] Inner membrane protein complexes, such as AgmU, propel the organism forward as these protein complexes function similar to the flagella complex of other motile organisms. These protein complexes, powered by a proton motive force, rotate within the membrane allowing cells to glide over surfaces.

Twitching

Built for use by many in the bacterial world, Twitching Motility is an important tool that bacteria use to move across moist surfaces. Twitching Motility uses a type IV pili that extends, tethers to a surface, and then pulls the bacteria forward. This allows for quicker growth across biofilms and fruiting bodies. Type IV pili is run by over forty genes that regulate this type of motility. [13] Myxococcus xanthus ability to use gliding motility to move is very similar to Pseudomonas aeruginosa twitching motility.[1] Pseudomonas aeruginosa is a very motile bacteria species but it has some drawbacks, in one experiment a team of researchers discovered that if they put pressure on colonies that exhibited the quickest motility it led to decreased production of biofilm formation but drastically increased rates of motility. They then compared their quickest strain to wild type species to see if there is a need for higher rates of motility in the environment but none came close. Overall increasing speeds did not increase the chance for survival in the long run. [14]

Related Research Articles

<span class="mw-page-title-main">Pilus</span> A proteinaceous hair-like appendage on the surface of bacteria

A pilus is a hair-like appendage found on the surface of many bacteria and archaea. The terms pilus and fimbria can be used interchangeably, although some researchers reserve the term pilus for the appendage required for bacterial conjugation. All conjugative pili are primarily composed of pilin – fibrous proteins, which are oligomeric.

<span class="mw-page-title-main">Flagellum</span> Cellular appendage functioning as locomotive or sensory organelle

A flagellum is a hairlike appendage that protrudes from certain plant and animal sperm cells, from fungal spores (zoospores), and from a wide range of microorganisms to provide motility. Many protists with flagella are known as flagellates.

<span class="mw-page-title-main">Myxobacteria</span> Order of bacteria

The myxobacteria are a group of bacteria that predominantly live in the soil and feed on insoluble organic substances. The myxobacteria have very large genomes relative to other bacteria, e.g. 9–10 million nucleotides except for Anaeromyxobacter and Vulgatibacter. One species of myxobacteria, Minicystis rosea, has the largest known bacterial genome with over 16 million nucleotides. The second largest is another myxobacteria Sorangium cellulosum.

<span class="mw-page-title-main">Motility</span> Ability to move using metabolic energy

Motility is the ability of an organism to move independently, using metabolic energy.

Microbial intelligence is the intelligence shown by microorganisms. The concept encompasses complex adaptive behavior shown by single cells, and altruistic or cooperative behavior in populations of like or unlike cells mediated by chemical signalling that induces physiological or behavioral changes in cells and influences colony structures.

<i>Myxococcus xanthus</i> Slime bacterium

Myxococcus xanthus is a gram-negative, bacillus species of myxobacteria that is typically found in the top-most layer of soil. These bacteria lack flagella; rather, they use pili for motility. M. xanthus is well-known for its predatory behavior on other microorganisms. These bacteria source carbon from lipids rather than sugars. They exhibit various forms of self-organizing behavior in response to environmental cues. Under normal conditions with abundant food, they exist as predatory, saprophytic single-species biofilm called a swarm, highlighting the importance of intercellular communication for these bacteria. Under starvation conditions, they undergo a multicellular development cycle.

<span class="mw-page-title-main">Bacteria</span> Domain of microorganisms

Bacteria are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria play a vital role in many stages of the nutrient cycle by recycling nutrients and the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in mutualistic, commensal and parasitic relationships with plants and animals. Most bacteria have not been characterised and there are many species that cannot be grown in the laboratory. The study of bacteria is known as bacteriology, a branch of microbiology.

A copiotroph is an organism found in environments rich in nutrients, particularly carbon. They are the opposite to oligotrophs, which survive in much lower carbon concentrations.

<span class="mw-page-title-main">Swarming motility</span>

Swarming motility is a rapid and coordinated translocation of a bacterial population across solid or semi-solid surfaces, and is an example of bacterial multicellularity and swarm behaviour. Swarming motility was first reported by Jorgen Henrichsen and has been mostly studied in genus Serratia, Salmonella, Aeromonas, Bacillus, Yersinia, Pseudomonas, Proteus, Vibrio and Escherichia.

<span class="mw-page-title-main">Bacterial motility</span> Ability of bacteria to move independently using metabolic energy

Bacterial motility is the ability of bacteria to move independently using metabolic energy. Most motility mechanisms that evolved among bacteria also evolved in parallel among the archaea. Most rod-shaped bacteria can move using their own power, which allows colonization of new environments and discovery of new resources for survival. Bacterial movement depends not only on the characteristics of the medium, but also on the use of different appendages to propel. Swarming and swimming movements are both powered by rotating flagella. Whereas swarming is a multicellular 2D movement over a surface and requires the presence of surfactants, swimming is movement of individual cells in liquid environments.

<span class="mw-page-title-main">Phototaxis</span>

Phototaxis is a kind of taxis, or locomotory movement, that occurs when a whole organism moves towards or away from a stimulus of light. This is advantageous for phototrophic organisms as they can orient themselves most efficiently to receive light for photosynthesis. Phototaxis is called positive if the movement is in the direction of increasing light intensity and negative if the direction is opposite.

<span class="mw-page-title-main">Gliding motility</span>

Gliding motility is a type of translocation used by microorganisms that is independent of propulsive structures such as flagella, pili, and fimbriae. Gliding allows microorganisms to travel along the surface of low aqueous films. The mechanisms of this motility are only partially known.

Vampirococcus is an informally described genus of ovoid Gram-negative bacteria, but the exact phylogeny remains to be determined. This predatory prokaryote was first described in 1983 by Esteve et al. as small, anaerobic microbe about 0.6 μm wide before being given the name of Vampirococcus in 1986 by Guerrero et al. This prokaryote is a freshwater obligate predator that preys specifically on various species of the photosynthetic purple sulfur bacterium, Chromatium. As an epibiont, Vampirococcus attaches to the cell surface of their prey and "sucks" out the cytoplasm using a specialized cytoplasmic bridge. They are commonly mentioned as an example of epibionts when discussing strategies employed by bacterial predators. This microbe still has yet to be classified based on genomic sequencing or 16S rRNA because it cannot be sustained long enough outside its natural environment to isolate a pure culture.

<span class="mw-page-title-main">Pxr sRNA</span>

Pxr sRNA is a regulatory RNA which downregulates genes responsible for the formation of fruiting bodies in Myxococcus xanthus. Fruiting bodies are aggregations of myxobacteria formed when nutrients are scarce, the fruiting bodies permit a small number of the aggregated colony to transform into stress-resistant spores.

Stigmatella aurantiaca is a member of myxobacteria, a group of gram-negative bacteria with a complex developmental life cycle.

<i>Myxococcus</i> Genus of bacteria

Myxococcus is a genus of bacteria in the family Myxococcaceae. Myxococci are Gram-negative, spore-forming, chemoorganotrophic, obligate aerobes. They are elongated rods with rounded or tapered ends, and they are nonflagellated. The cells move by gliding and can predate other bacteria. The genus has been isolated from soil.

<span class="mw-page-title-main">Twitching motility</span> Form of crawling bacterial motility

Twitching motility is a form of crawling bacterial motility used to move over surfaces. Twitching is mediated by the activity of hair-like filaments called type IV pili which extend from the cell's exterior, bind to surrounding solid substrates, and retract, pulling the cell forwards in a manner similar to the action of a grappling hook. The name twitching motility is derived from the characteristic jerky and irregular motions of individual cells when viewed under the microscope. It has been observed in many bacterial species, but is most well studied in Pseudomonas aeruginosa, Neisseria gonorrhoeae and Myxococcus xanthus. Active movement mediated by the twitching system has been shown to be an important component of the pathogenic mechanisms of several species.

<span class="mw-page-title-main">Marine prokaryotes</span> Marine bacteria and marine archaea

Marine prokaryotes are marine bacteria and marine archaea. They are defined by their habitat as prokaryotes that live in marine environments, that is, in the saltwater of seas or oceans or the brackish water of coastal estuaries. All cellular life forms can be divided into prokaryotes and eukaryotes. Eukaryotes are organisms whose cells have a nucleus enclosed within membranes, whereas prokaryotes are the organisms that do not have a nucleus enclosed within a membrane. The three-domain system of classifying life adds another division: the prokaryotes are divided into two domains of life, the microscopic bacteria and the microscopic archaea, while everything else, the eukaryotes, become the third domain.

<span class="mw-page-title-main">Run-and-tumble motion</span> Type of bacterial motion

Run-and-tumble motion is a movement pattern exhibited by certain bacteria and other microscopic agents. It consists of an alternating sequence of "runs" and "tumbles": during a run, the agent propels itself in a fixed direction, and during a tumble, it remains stationary while it reorients itself in preparation for the next run.

<span class="mw-page-title-main">Adventurous motility</span>

Adventurous motility is as a type of gliding motility; unlike most motility mechanisms, adventurous motility does not involve a flagellum. Gliding motility usually involves swarms of bacteria; however, adventurous motility is practiced by individual cells. This gliding is hypothesized to occur via assembly of a type IV secretion system and the extrusion of a polysaccharide slime, or by use of a series of adhesion complexes. The majority of research on adventurous motility has focused on the species, Myxococcus xanthus. The earliest of this research is attributed to Jonathan Hodgkin and Dale Kaiser.

References

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