Burkholderia cenocepacia

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Burkholderia cenocepacia
Burkholderia cepacia.jpg
Electron micrograph of Burkholderia cepacia
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Domain: Bacteria
Phylum: Pseudomonadota
Class: Betaproteobacteria
Order: Burkholderiales
Family: Burkholderiaceae
Genus: Burkholderia
Species:
B. cenocepacia
Binomial name
Burkholderia cenocepacia
Vandamme et al. 2003

Burkholderia cenocepacia is a Gram-negative, rod-shaped bacterium that is commonly found in soil and water environments and may also be associated with plants and animals, particularly as a human pathogen. [1] It is one of over 20 species in the Burkholderia cepacia complex (Bcc) and is notable due to its virulence factors and inherent antibiotic resistance that render it a prominent opportunistic pathogen responsible for life-threatening, nosocomial infections in immunocompromised patients, such as those with cystic fibrosis or chronic granulomatous disease. [2] The quorum sensing systems CepIR and CciIR regulate the formation of biofilms and the expression of virulence factors such as siderophores and proteases. [3] Burkholderia cenocepacia may also cause disease in plants, such as in onions [4] [5] and bananas. [6] Additionally, some strains serve as plant growth-promoting rhizobacteria. [7]

Contents

Taxonomy

Within the Burkholderia genus, the Burkholderia cepacia complex contains over 20 related species that cause opportunistic infections and possess antibiotic resistance. [8] Burkholderia cepacia was originally defined as a single species, but it is now one of several species in the Bcc. [9] Although closely related, the species within the Bcc have differing severity of pathogenicity, and B. cenocepacia is one of the most intensively studied due to its higher pathogenicity and antibiotic resistance compared to other species in the complex. [8] Exchange of genetic material between species of the Bcc has resulted in a reticulated phylogeny that presents an obstacle to diagnostic classification at the species-level. [8] Because of this phenotypic overlap between species, previous nomenclature of Bcc species involved genomovar terms, with Burkholderia cenocepacia categorized as genomovar III of the Bcc. [6] [10] Within the categorization as genomovar III, there are 4 phylogenetic lineage groups: IIIA, IIIB, IIIC, and IIID. [11] No IIIC isolates have been found in studies on the natural environment, whereas all IIID isolates studied have been in clinical isolates of B. cenocepacia. [12]

Microbiology

The strong environmental protection response of B. cenocepacia is attributed to the biofilm formed by groups of the organism. [13] This biofilm contains exopolysaccharides that strengthen the bacterium's resistance to antibiotics and contribute to the bacteria's virulence. It is made up of a highly branched polysaccharide unit with one glucose, one glucuronic acid, one mannose, one rhamnose, and three galactose molecules. This species in the Burkholderia cepacia complex has also created another polysaccharide with one 3-deoxy-d-manno-2-octulosonic acid and three galactose molecules. [14] The biofilm exopolysaccharides act as a barrier to neutrophils from human immune resistance systems, undermining the neutrophil defense action by inhibiting neutrophil chemotaxis and scavenging reactive oxygen species, which are bactericidal products produced by neutrophils to destroy bacteria. [15]

Genome

B. cenocepacia's genome consists of three circular chromosomes and one plasmid. Chromosome 1 contains 3.87 Mb, chromosome 2 contains 3.22 Mb, and chromosome 3 contains 0.88 Mb. The plasmid is approximately 0.09 Mb. [16] Chromosome 3 has also been characterized as a large plasmid, or megaplasmid (pC3); unlike chromosomes 2 and 3, it does not contain essential housekeeping genes, instead coding for accessory functions such as virulence and resistance to stress. [17] [18] In addition to the multireplicon structure, the genome contains several insertion sequences and can rapidly mutate during infections, which contribute to B. cenocepacia's unique adaptability and ability to acquire diverse catabolic functions. [19] [20]

Environments

Burkholderia cenocepacia has been found to thrive in primarily microaerophilic conditions, which consist of little to no oxygen. [21] Experimental studies conducted on the growth of B. cenocepacia in environments akin to the human lungs demonstrated the pathogen's increased success in microaerophilic environments over aerophilic settings. [21] In environments with little available iron such as the lungs of a cystic fibrosis patient, Burkholderia cenocepacia secretes siderophores, molecules that bind to iron and transport them to the bacteria. [22] Out of the four types of siderophores produced by the Bcc, B. cenocepacia produces three: ornibactin, pyochelin, and salicylic acid (SA). Ornibactin is the most important iron uptake system and can sustain the bacteria in an iron-deficient environment even without the production of functioning pyochelin or SA. [23]

B. cenocepacia has been demonstrated to colonize an array of ecological niches with diverse lifestyles. The ability to utilize a wide range of carbon sources accompanies the ability of Bcc species to be efficient with plant-growth promotion, bioremediation, and biocontrol. [12] [24] High potential of Bcc species, including B. cenocepacia, as a biocontrol of plant-growth promoting agents has been demonstrated; however, the mechanisms that support this are not known. [12] In a bioremediation context, various Bcc strains are suggested to hold high potential to remediate environments contaminated with toxic compounds, including halogenated compounds. [12]

In addition, B. cenocepacia has been found to exist in the rhizosphere, plants, soil, water, and animals. [12] In fact, it was found to have an endophytic lifestyle when recovered from plant material, indicating that it has endosymbiotic characteristics. [12] Burkholderia cenocepacia was the dominant genomovar recovered in a study of bacteria in the rhizosphere of maize in China, pointing to endosymbiotic attributes with plants in soil. [25] However, B. cenocepacia also demonstrated phytopathogenic properties in causing fingertip rot in bananas. [6]

Quorum sensing

One kind of cell-to-cell communication employed by B. cenocepacia is quorum sensing, which is the detection of fluctuations in cell density and usage of this information to regulate functions such as the formation of biofilms. Like other Gram-negative bacteria, B. cenocepacia produces acyl-homoserine lactones (AHLs), signaling molecules that in members of the Burkholderia cepacia complex specifically are encoded by two systems–the CepIR system, which is highly conserved in the Bcc, and the CciIR system. [26] The two AHL-mediated QS systems, CepIR and CciIR, regulate each other; the CepR protein is required for the transcription of the cciIR operon, while the CciR protein represses transcription of cepI. The CciIR system can also negatively regulate the CepIR system through the production of C6-HSL, a type of AHL produced primarily by CciI proteins that inhibits the activity of CepR proteins. [26] [27] The bacterium also uses cis-2-dodecenoic acid signals, which are known as Burkholderia diffusible signal factors (BDSF) because they were first identified in Burkholderia cenocepacia. [28]

Motility

Burkholderia cenocepacia has the ability to swim and swarm inside the body. It has a polar flagella and produces a surfactant. These characteristics are necessary for the species to have motility in an agar medium. The surfactant produced by Burkholderia cenocepacia allows other pathogenic bacteria in the lungs to have motility. This means that the presence of Burkholderia cenocepacia is necessary for swarms of bacteria to coexist and cooperate in the lungs. [29]

Pathogenicity

Burkholderia cenocepacia is an opportunistic pathogen that commonly infects immunocompromised patients, especially those with cystic fibrosis, and is often lethal. [30] In cystic fibrosis, it can cause "cepacia syndrome," which is characterized by a rapidly progressive fever, uncontrolled bronchopneumonia, weight loss, and in some cases, death. A review of B. cenocepacia in respiratory infections of cystic fibrosis patients stated that "one of the most threatening pathogens in [cystic fibrosis] is Burkholderia cenocepacia, a member of a bacterial group collectively referred to as the Burkholderia cepacia complex." [31] Twenty-four small RNAs were identified using RNA-binding properties of the Hfq protein during the exponential growth phases. [32] sRNAs identified in Burkholderia cenocepacia KC-0 were upregulated under iron depletion and oxidative stress. [33] Burkholderia cenocepacia encodes two RNA chaperone proteins that assist sRNAs in binding to mRNA, Hfq and Hfq2. Both are required for maximum virulence and resistance against stressors such as acidic pH, high temperatures, osmotic stress, and oxidative stress. [34] [35] Burkholderia cenocepacia produces a toxin called double-stranded DNA deaminase A (DddA) made by the bacterium that converts DNA base cytosine to uracil. [36] Because uracil, which is not commonly found in DNA, behaves like a thymidine, the enzymes that replicate the cell’s DNA copy it as a thymidine, effectively converting a cytosine in the genome sequence to a thymidine. This has reportedly been used for the first gene-editing of mitochondria – for which a team at the Broad Institute developed a new kind of CRISPR-free base editor, called DdCBE, using the toxin. [37] [38] [39]

See also: Burkholderia thailandensis sRNA

Antibiotic resistance

The structural factors that contribute to the antibiotic resistance of B. cenocepacia include: an impermeable outer membrane, an efflux pump mechanism, and the production of a beta-lactamase. [40] This microbe challenges infection prevention as it is resistant to some disinfectants and antiseptics. It can survive on surfaces, including human skin and mucosal surfaces for an extended period of time. [41]

Virulence

Virulence in Burkholderia cenocepacia is widely attributed to biofilm formation, siderophore production, and QS signaling - each of which affect how the species adapts in various environmental conditions. [22] B. cenocepacia's ability to adapt to host environments contributes to chronic opportunistic infections and bacterial persistence. [19] Several strains are noted as "epidemic strains" due to increased transmission capability and patient-to-patient transmission. [12] The ET12 strain was found to have a "cable pilus," which enables greater adhesion of bacteria to epithelial cells. [12]

In human airway epithelial cells, the invasion pathway utilized by the BC-7 strain of B. cenocepacia is largely the result of the strain's biofilm formation. [42] In general, both environmental and clinical strains of B. cenocepacia are able to form biofilms; however, the ability to do so is greater in clinical strains. [43] The H111 strain of Burkholderia cenocepacia forms biofilms on pea roots, for example. [44] Quorum signaling (QS) affects the ability of B. cenocepacia to develop biofilms, in addition to the motility abilities. [45] In addition, quorum signaling controls a variety of cellular processes, such as extracellular proteases, polygalacturonase, and the production of siderophores. [45]

Cystic fibrosis

Burkholderia cenocepacia is one of over twenty bacteria in the Burkholderia cepacia complex (Bcc), and among these species, it is a dominant bacteria associated with cystic fibrosis. B. cenocepacia has such high transmissibility that it has spread across continents, including Europe and Canada, between cystic fibrosis patients at epidemic levels. [22] Patients with cystic fibrosis are threatened most by opportunistic pathogens. [22] Based on the distribution of Bcc species in sample cystic fibrosis patient populations, B. cenocepacia claims between 45.6% and 91.8% of all infections caused by the Bcc complex. [22] Compared to other infectious agents found in cystic fibrosis patients, the Bcc complex demonstrates the greatest association with increased morbidity and mortality. [46] Compared to other species in the Bcc complex, B. cenocepacia was shown to possibly accelerate BMI decline and FEV1 (forced expiration) at the greatest rate, leading to worse prognoses for cystic fibrosis patients. [46] The Bcc complex consists of genomovars, which are species characterized to be phylogenetically close, though distinct from each other. [47] In cystic fibrosis infections, it is common for only one of the known nine genomovars to induce an infection. [47] Overall, in patients with cystic fibrosis, the genomovar status of the Bcc has a significant influence on the success of clinical interventions, as well as the temporal progression of the condition. [48]

Applications

Biotechnology

Given the opportunistic nature of the Bcc complex and B. cenocepacia, the severity of respiratory infections is considered to be a significant conflict for applications in biotechnology. [12]

Agriculture

To increase soil health, plant-growth promoting rhizobacteria (PGPR) are used in the agricultural industry to create bio-organic fertilizers. [49] A current challenge is identifying which bacterial species are optimal at stimulating plant growth in bio-organic fertilizers. Creating bio-organic fertilizers has been increasingly successful with the use of plant-growth promoting rhizobacteria mixed with organic substrates. [49] B. cenocepacia has various PGPR traits like phosphate solubilization that make it well-suited to promote growth. With the addition of solid-state fermentation technology, creating bio-organic fertilizers was highly successful by incorporating B. cenocepacia with high protein content agricultural wastes. [49]

Related Research Articles

<span class="mw-page-title-main">Biofilm</span> Aggregation of bacteria or cells on a surface

A biofilm is a syntrophic community of microorganisms in which cells stick to each other and often also to a surface. These adherent cells become embedded within a slimy extracellular matrix that is composed of extracellular polymeric substances (EPSs). The cells within the biofilm produce the EPS components, which are typically a polymeric combination of extracellular polysaccharides, proteins, lipids and DNA. Because they have a three-dimensional structure and represent a community lifestyle for microorganisms, they have been metaphorically described as "cities for microbes".

<i>Burkholderia</i> Genus of bacteria

Burkholderia is a genus of Pseudomonadota whose pathogenic members include the Burkholderia cepacia complex, which attacks humans and Burkholderia mallei, responsible for glanders, a disease that occurs mostly in horses and related animals; Burkholderia pseudomallei, causative agent of melioidosis; and Burkholderia cepacia, an important pathogen of pulmonary infections in people with cystic fibrosis (CF). Burkholderia species is also found in marine environments. S.I. Paul et al. (2021) isolated and characterized Burkholderia cepacia from marine sponges of the Saint Martin's Island of the Bay of Bengal, Bangladesh.

<i>Enterobacter</i> Genus of bacteria

Enterobacter is a genus of common Gram-negative, facultatively anaerobic, rod-shaped, non-spore-forming bacteria of the family Enterobacteriaceae. Cultures are found in soil, water, sewage, feces and gut environments. It is the type genus of the order Enterobacterales. Several strains of these bacteria are pathogenic and cause opportunistic infections in immunocompromised hosts and in those who are on mechanical ventilation. The urinary and respiratory tracts are the most common sites of infection. The genus Enterobacter is a member of the coliform group of bacteria. It does not belong to the fecal coliforms group of bacteria, unlike Escherichia coli, because it is incapable of growth at 44.5 °C in the presence of bile salts. Some of them show quorum sensing properties.

<span class="mw-page-title-main">Opportunistic infection</span> Infection caused by pathogens that take advantage of an opportunity not normally available

An opportunistic infection is an infection caused by pathogens that take advantage of an opportunity not normally available. These opportunities can stem from a variety of sources, such as a weakened immune system, an altered microbiome, or breached integumentary barriers. Many of these pathogens do not necessarily cause disease in a healthy host that has a non-compromised immune system, and can, in some cases, act as commensals until the balance of the immune system is disrupted. Opportunistic infections can also be attributed to pathogens which cause mild illness in healthy individuals but lead to more serious illness when given the opportunity to take advantage of an immunocompromised host.

<i>Pseudomonas aeruginosa</i> Species of bacterium

Pseudomonas aeruginosa is a common encapsulated, Gram-negative, aerobic–facultatively anaerobic, rod-shaped bacterium that can cause disease in plants and animals, including humans. A species of considerable medical importance, P. aeruginosa is a multidrug resistant pathogen recognized for its ubiquity, its intrinsically advanced antibiotic resistance mechanisms, and its association with serious illnesses – hospital-acquired infections such as ventilator-associated pneumonia and various sepsis syndromes. P. aeruginosa is able to selectively inhibit various antibiotics from penetrating its outer membrane - and has high resistance to several antibiotics. According to the World Health Organization P. aeruginosa poses one of the greatest threats to humans in terms of antibiotic resistance.

<i>Staphylococcus epidermidis</i> Species of bacterium

Staphylococcus epidermidis is a Gram-positive bacterium, and one of over 40 species belonging to the genus Staphylococcus. It is part of the normal human microbiota, typically the skin microbiota, and less commonly the mucosal microbiota and also found in marine sponges. It is a facultative anaerobic bacteria. Although S. epidermidis is not usually pathogenic, patients with compromised immune systems are at risk of developing infection. These infections are generally hospital-acquired. S. epidermidis is a particular concern for people with catheters or other surgical implants because it is known to form biofilms that grow on these devices. Being part of the normal skin microbiota, S. epidermidis is a frequent contaminant of specimens sent to the diagnostic laboratory.

<i>Burkholderia cepacia</i> complex Species of bacterium

Burkholderia cepacia complex (BCC) is a species complex consisting of Burkholderia cepacia and at least 20 different biochemically similar species of Gram-negative bacteria. They are catalase-producing and lactose-nonfermenting. Members of BCC are opportunistic human pathogens that most often cause pneumonia in immunocompromised individuals with underlying lung disease. Patients with sickle-cell haemoglobinopathies are also at risk. The species complex also attacks young onion and tobacco plants, and displays a remarkable ability to digest oil.

<i>Stenotrophomonas maltophilia</i> Species of bacterium

Stenotrophomonas maltophilia is an aerobic, nonfermentative, Gram-negative bacterium. It is an uncommon bacterium and human infection is difficult to treat. Initially classified as Bacterium bookeri, then renamed Pseudomonas maltophilia, S. maltophilia was also grouped in the genus Xanthomonas before eventually becoming the type species of the genus Stenotrophomonas in 1993.

<i>Burkholderia pseudomallei</i> Species of bacterium

Burkholderia pseudomallei is a Gram-negative, bipolar, aerobic, motile rod-shaped bacterium. It is a soil-dwelling bacterium endemic in tropical and subtropical regions worldwide, particularly in Thailand and northern Australia. It was reported in 2008 that there had been an expansion of the affected regions due to significant natural disasters, and it could be found in Southern China, Hong Kong, and countries in the Americas. B. pseudomallei, amongst other pathogens, has been found in monkeys imported into the United States from Asia for laboratory use, posing a risk that the pathogen could be introduced into the country.

Burkholderia gladioli is a species of aerobic gram-negative rod-shaped bacteria that causes disease in both humans and plants. It can also live in symbiosis with plants and fungi and is found in soil, water, the rhizosphere, and in the microbiome of many animals. It was formerly known as Pseudomonas marginata.

<span class="mw-page-title-main">Lactonase</span> Class of enzymes

Lactonase (EC 3.1.1.81, acyl-homoserine lactonase; systematic name N-acyl-L-homoserine-lactone lactonohydrolase) is a metalloenzyme, produced by certain species of bacteria, which targets and inactivates acylated homoserine lactones (AHLs). It catalyzes the reaction

Walter Hagemeyer Burkholder was an American plant pathologist who helped establish the role of bacteria as plant pathogens. He was awarded a Ph.D. by Cornell University in 1917 and subsequently appointed as professor of plant pathology.

Pathogenomics is a field which uses high-throughput screening technology and bioinformatics to study encoded microbe resistance, as well as virulence factors (VFs), which enable a microorganism to infect a host and possibly cause disease. This includes studying genomes of pathogens which cannot be cultured outside of a host. In the past, researchers and medical professionals found it difficult to study and understand pathogenic traits of infectious organisms. With newer technology, pathogen genomes can be identified and sequenced in a much shorter time and at a lower cost, thus improving the ability to diagnose, treat, and even predict and prevent pathogenic infections and disease. It has also allowed researchers to better understand genome evolution events - gene loss, gain, duplication, rearrangement - and how those events impact pathogen resistance and ability to cause disease. This influx of information has created a need for bioinformatics tools and databases to analyze and make the vast amounts of data accessible to researchers, and it has raised ethical questions about the wisdom of reconstructing previously extinct and deadly pathogens in order to better understand virulence.

Burkholderia contaminans is a gram-negative, bacterium from the genus of Burkholderia and the family of Burkholderiaceae and belongs to the Burkholderia cepacia complex, which was isolated from cystic fibrosis patients in Argentina. Burkholderia acidipaludis can cause biliary sepsis.

Pandoraea pulmonicola is a Gram-negative, non-spore-forming, motile bacterium with a single polar flagellum, of the genus Pandoraea. P. pulmonicola has been isolated from respiratory samples of patients with cystic fibrosis and other respiratory diseases. P. pulmonicola is a part of the Burkholderia cepacia complex, which is a group of bacteria commonly associated with infections in individuals with compromised immune systems.

Staphylococcus pseudintermedius is a gram positive coccus bacteria of the genus Staphylococcus found worldwide. It is primarily a pathogen for domestic animals, but has been known to affect humans as well. S. pseudintermedius is an opportunistic pathogen that secretes immune modulating virulence factors, has many adhesion factors, and the potential to create biofilms, all of which help to determine the pathogenicity of the bacterium. Diagnoses of Staphylococcus pseudintermedius have traditionally been made using cytology, plating, and biochemical tests. More recently, molecular technologies like MALDI-TOF, DNA hybridization and PCR have become preferred over biochemical tests for their more rapid and accurate identifications. This includes the identification and diagnosis of antibiotic resistant strains.

Everett Peter Greenberg is an American microbiologist. He is the inaugural Eugene and Martha Nester Professor of Microbiology at the Department of Microbiology of the University of Washington School of Medicine. He is best known for his research on quorum sensing, and has received multiple awards for his work.

Ornibactin is a siderophore, or small iron-binding compound secreted by bacteria to transport iron into the cell. Ornibactin is produced by Burkholderia cenocepacia under iron-deficient conditions. B. cenocepacia is known to opportunistically infect humans, specifically ones suffering from cystic fibrosis.

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References

  1. ↑ O'Grady EP, Sokol PA (2011-12-09). "Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment". Frontiers in Cellular and Infection Microbiology. 1: 15. doi: 10.3389/fcimb.2011.00015 . PMC   3417382 . PMID   22919581.
  2. ↑ Lauman P, Dennis JJ (July 2021). "Advances in Phage Therapy: Targeting the Burkholderia cepacia Complex". Viruses. 13 (7): 1331. doi: 10.3390/v13071331 . PMC   8310193 . PMID   34372537.
  3. ↑ Scoffone VC, Chiarelli LR, Makarov V, Brackman G, Israyilova A, Azzalin A, et al. (September 2016). "Discovery of new diketopiperazines inhibiting Burkholderia cenocepacia quorum sensing in vitro and in vivo". Scientific Reports. 6 (1): 32487. doi:10.1038/srep32487. PMC   5007513 . PMID   27580679.
  4. ↑ da Silva PH, de Assunção EF, da Silva Velez L, Dos Santos LN, de Souza EB, da Gama MA (December 2021). "Biofilm formation by strains of Burkholderia cenocepacia lineages IIIA and IIIB and B. gladioli pv. alliicola associated with onion bacterial scale rot". Brazilian Journal of Microbiology. 52 (4): 1665–1675. doi:10.1007/s42770-021-00564-6. PMC   8578472 . PMID   34351603.
  5. ↑ Jacobs JL, Fasi AC, Ramette A, Smith JJ, Hammerschmidt R, Sundin GW (May 2008). "Identification and onion pathogenicity of Burkholderia cepacia complex isolates from the onion rhizosphere and onion field soil". Applied and Environmental Microbiology. 74 (10): 3121–3129. Bibcode:2008ApEnM..74.3121J. doi:10.1128/AEM.01941-07. PMC   2394932 . PMID   18344334.
  6. 1 2 3 Lee YA, Chan CW (February 2007). "Molecular Typing and Presence of Genetic Markers Among Strains of Banana Finger-Tip Rot Pathogen, Burkholderia cenocepacia, in Taiwan". Phytopathology. 97 (2): 195–201. doi: 10.1094/PHYTO-97-2-0195 . PMID   18944375.
  7. ↑ You M, Fang S, MacDonald J, Xu J, Yuan ZC (March 2020). "Isolation and characterization of Burkholderia cenocepacia CR318, a phosphate solubilizing bacterium promoting corn growth". Microbiological Research. 233: 126395. doi:10.1016/j.micres.2019.126395. PMID   31865096. S2CID   209445961.
  8. 1 2 3 Wang H, Cissé OH, Bolig T, Drake SK, Chen Y, Strich JR, et al. (October 2020). "A Phylogeny-Informed Proteomics Approach for Species Identification within the Burkholderia cepacia Complex". Journal of Clinical Microbiology. 58 (11): e01741–20. doi:10.1128/JCM.01741-20. PMC   7587091 . PMID   32878952.
  9. ↑ Tavares M, Kozak M, Balola A, Sá-Correia I (June 2020). "Burkholderia cepacia Complex Bacteria: a Feared Contamination Risk in Water-Based Pharmaceutical Products". Clinical Microbiology Reviews. 33 (3): e00139–19. doi:10.1128/CMR.00139-19. PMC   7194853 . PMID   32295766.
  10. ↑ Lipuma JJ (November 2005). "Update on the Burkholderia cepacia complex". Current Opinion in Pulmonary Medicine. 11 (6): 528–533. doi:10.1097/01.mcp.0000181475.85187.ed. PMID   16217180. S2CID   19117513.
  11. ↑ Vandamme P, Holmes B, Coenye T, Goris J, Mahenthiralingam E, LiPuma JJ, Govan JR (March 2003). "Burkholderia cenocepacia sp. nov.--a new twist to an old story". Research in Microbiology. 154 (2): 91–96. doi: 10.1016/S0923-2508(03)00026-3 . PMID   12648723.
  12. 1 2 3 4 5 6 7 8 9 Vial L, Chapalain A, Groleau MC, Déziel E (January 2011). "The various lifestyles of the Burkholderia cepacia complex species: a tribute to adaptation". Environmental Microbiology. 13 (1): 1–12. doi: 10.1111/j.1462-2920.2010.02343.x . PMID   20880095.
  13. ↑ Alshraiedeh NH, Higginbotham S, Flynn PB, Alkawareek MY, Tunney MM, Gorman SP, et al. (June 2016). "Eradication and phenotypic tolerance of Burkholderia cenocepacia biofilms exposed to atmospheric pressure non-thermal plasma". International Journal of Antimicrobial Agents. 47 (6): 446–450. doi:10.1016/j.ijantimicag.2016.03.004. PMID   27179816. B. cenocepacia can spread from person to person and exhibits intrinsic broad-spectrum antibiotic resistance
  14. ↑ Chiarini L, Cescutti P, Drigo L, Impallomeni G, Herasimenka Y, Bevivino A, et al. (August 2004). "Exopolysaccharides produced by Burkholderia cenocepacia recA lineages IIIA and IIIB". Journal of Cystic Fibrosis. 3 (3): 165–172. doi: 10.1016/j.jcf.2004.04.004 . PMID   15463903.
  15. ↑ Bylund J, Burgess LA, Cescutti P, Ernst RK, Speert DP (February 2006). "Exopolysaccharides from Burkholderia cenocepacia inhibit neutrophil chemotaxis and scavenge reactive oxygen species". The Journal of Biological Chemistry. 281 (5): 2526–2532. doi: 10.1074/jbc.M510692200 . PMID   16316987. We showed that EPS from a clinical B. cenocepacia isolate interfered with the function of human neutrophils in vitro; it inhibited chemotaxis and production of reactive oxygen species (ROS), both essential components of innate neutrophil-mediated host defenses
  16. ↑ O'Grady EP, Sokol PA (2011). "Burkholderia cenocepacia differential gene expression during host-pathogen interactions and adaptation to the host environment". Frontiers in Cellular and Infection Microbiology. 1: 15. doi: 10.3389/fcimb.2011.00015 . PMC   3417382 . PMID   22919581.
  17. ↑ Agnoli K, Frauenknecht C, Freitag R, Schwager S, Jenul C, Vergunst A, et al. (February 2014). "The third replicon of members of the Burkholderia cepacia Complex, plasmid pC3, plays a role in stress tolerance". Applied and Environmental Microbiology. 80 (4): 1340–1348. doi:10.1128/AEM.03330-13. PMC   3911052 . PMID   24334662.
  18. ↑ Agnoli K, Schwager S, Uehlinger S, Vergunst A, Viteri DF, Nguyen DT, et al. (January 2012). "Exposing the third chromosome of Burkholderia cepacia complex strains as a virulence plasmid". Molecular Microbiology. 83 (2): 362–378. doi: 10.1111/j.1365-2958.2011.07937.x . PMID   22171913. S2CID   12668288.
  19. 1 2 Loutet, Slade A.; Valvano, Miguel A. (October 2010). "A Decade of Burkholderia cenocepacia Virulence Determinant Research". Infection and Immunity. 78 (10): 4088–4100. doi:10.1128/IAI.00212-10. ISSN   0019-9567. PMC   2950345 . PMID   20643851.
  20. ↑ Lessie, T. G.; Hendrickson, W.; Manning, B. D.; Devereux, R. (1996-11-01). "Genomic complexity and plasticity of Burkholderia cepacia". FEMS Microbiology Letters. 144 (2–3): 117–128. doi: 10.1111/j.1574-6968.1996.tb08517.x . ISSN   0378-1097. PMID   8900054. S2CID   46192579.
  21. 1 2 Coutinho CP, de Carvalho CC, Madeira A, Pinto-de-Oliveira A, Sá-Correia I (July 2011). Payne SM (ed.). "Burkholderia cenocepacia phenotypic clonal variation during a 3.5-year colonization in the lungs of a cystic fibrosis patient". Infection and Immunity. 79 (7): 2950–2960. doi:10.1128/IAI.01366-10. PMC   3191963 . PMID   21536796.
  22. 1 2 3 4 5 Drevinek P, Mahenthiralingam E (July 2010). "Burkholderia cenocepacia in cystic fibrosis: epidemiology and molecular mechanisms of virulence". Clinical Microbiology and Infection. 16 (7): 821–830. doi: 10.1111/j.1469-0691.2010.03237.x . PMID   20880411.
  23. ↑ Visser MB, Majumdar S, Hani E, Sokol PA (May 2004). "Importance of the ornibactin and pyochelin siderophore transport systems in Burkholderia cenocepacia lung infections". Infection and Immunity. 72 (5): 2850–2857. doi:10.1128/IAI.72.5.2850-2857.2004. PMC   387874 . PMID   15102796.
  24. ↑ Mahenthiralingam E, Baldwin A, Dowson CG (June 2008). "Burkholderia cepacia complex bacteria: opportunistic pathogens with important natural biology". Journal of Applied Microbiology. 104 (6): 1539–1551. doi:10.1111/j.1365-2672.2007.03706.x. PMID   18217926. S2CID   23275498.
  25. ↑ Zhang L, Xie G (January 2007). "Diversity and distribution of Burkholderia cepacia complex in the rhizosphere of rice and maize". FEMS Microbiology Letters. 266 (2): 231–5. doi: 10.1111/j.1574-6968.2006.00530.x . PMID   17233735.
  26. 1 2 Suppiger A, Schmid N, Aguilar C, Pessi G, Eberl L (July 2013). "Two quorum sensing systems control biofilm formation and virulence in members of the Burkholderia cepacia complex". Virulence. 4 (5): 400–409. doi:10.4161/viru.25338. PMC   3714132 . PMID   23799665.
  27. ↑ O'Grady EP, Viteri DF, Malott RJ, Sokol PA (September 2009). "Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia". BMC Genomics. 10 (1): 441. doi: 10.1186/1471-2164-10-441 . PMC   2753556 . PMID   19761612.
  28. ↑ Wang M, Li X, Song S, Cui C, Zhang LH, Deng Y (February 2022). Alexandre G (ed.). "The cis-2-Dodecenoic Acid (BDSF) Quorum Sensing System in Burkholderia cenocepacia". Applied and Environmental Microbiology. 88 (4): e0234221. doi:10.1128/aem.02342-21. PMC   8863054 . PMID   34985987.
  29. ↑ Morin C, Landry M, Groleau MC, Déziel E (August 2022). Tamaki H (ed.). "Surface Motility Favors Codependent Interaction between Pseudomonas aeruginosa and Burkholderia cenocepacia". mSphere. 7 (4): e0015322. doi:10.1128/msphere.00153-22. PMC   9429929 . PMID   35862793.
  30. ↑ Csávás M, Malinovská L, Perret F, Gyurkó M, Illyés ZT, Wimmerová M, Borbás A (January 2017). "Tri- and tetravalent mannoclusters cross-link and aggregate BC2L-A lectin from Burkholderia cenocepacia". Carbohydrate Research. 437. Elsevier: 1–8. doi:10.1016/j.carres.2016.11.008. hdl: 2437/239138 . PMID   27871013. It is recognized as an opportunistic human pathogen causing lung infections in immunocompromised individuals, especially in cystic fibrosis patients, with significant mortality and morbidity
  31. ↑ Drevinek P, Mahenthiralingam E (July 2010). "Burkholderia cenocepacia in cystic fibrosis: epidemiology and molecular mechanisms of virulence". Clinical Microbiology and Infection. 16 (7): 821–830. doi: 10.1111/j.1469-0691.2010.03237.x . PMID   20880411.
  32. ↑ Ramos CG, Grilo AM, da Costa PJ, Leitão JH (February 2013). "Experimental identification of small non-coding regulatory RNAs in the opportunistic human pathogen Burkholderia cenocepacia J2315". Genomics. 101 (2): 139–148. doi: 10.1016/j.ygeno.2012.10.006 . PMID   23142676.
  33. ↑ Ghosh S, Dureja C, Khatri I, Subramanian S, Raychaudhuri S, Ghosh S (December 2017). "Identification of novel small RNAs in Burkholderia cenocepacia KC-01 expressed under iron limitation and oxidative stress conditions". Microbiology. 163 (12): 1924–1936. doi: 10.1099/mic.0.000566 . PMID   29099689.
  34. ↑ Ramos CG, Sousa SA, Grilo AM, Feliciano JR, Leitão JH (April 2011). "The second RNA chaperone, Hfq2, is also required for survival under stress and full virulence of Burkholderia cenocepacia J2315". Journal of Bacteriology. 193 (7): 1515–1526. doi:10.1128/JB.01375-10. PMC   3067662 . PMID   21278292. (Retracted, see doi:10.1128/JB.02242-14, PMID   25319526,  Retraction Watch . If this is an intentional citation to a retracted paper, please replace {{ retracted |...}} with {{ retracted |...|intentional=yes}}.)
  35. ↑ Sousa SA, Feliciano JR, Pita T, Guerreiro SI, Leitão JH (January 2017). "Burkholderia cepacia Complex Regulation of Virulence Gene Expression: A Review". Genes. 8 (1): 43. doi: 10.3390/genes8010043 . PMC   5295037 . PMID   28106859.
  36. ↑ de Moraes MH, Hsu F, Huang D, Bosch DE, Zeng J, Radey MC, Simon N, Ledvina HE, Frick JP, Wiggins PA, Peterson SB, Mougous JD (January 2021). "An interbacterial DNA deaminase toxin directly mutagenizes surviving target populations". eLife. 10. doi: 10.7554/eLife.62967 . PMC   7901873 . PMID   33448264.
  37. ↑ "The powerhouses inside cells have been gene-edited for the first time". New Scientist. 8 July 2020. Retrieved 12 July 2020.
  38. ↑ McRae M (10 July 2020). "For The First Time, Scientists Find a Way to Make Targeted Edits to Mitochondrial DNA". Science Alert.
  39. ↑ Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, et al. (July 2020). "A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing". Nature. 583 (7817): 631–637. Bibcode:2020Natur.583..631M. doi:10.1038/s41586-020-2477-4. PMC   7381381 . PMID   32641830.
  40. ↑ You M, Fang S, MacDonald J, Xu J, Yuan ZC (March 2020). "Isolation and characterization of Burkholderia cenocepacia CR318, a phosphate solubilizing bacterium promoting corn growth". Microbiological Research. 233: 126395. doi:10.1016/j.micres.2019.126395. PMID   31865096. S2CID   209445961.
  41. ↑ Loeven NA, Perault AI, Cotter PA, Hodges CA, Schwartzman JD, Hampton TH, Bliska JB (October 2021). "The Burkholderia cenocepacia Type VI Secretion System Effector TecA Is a Virulence Factor in Mouse Models of Lung Infection". mBio. 12 (5): e0209821. doi:10.1128/mBio.02098-21. PMC   8546862 . PMID   34579569.
  42. ↑ Schwab U, Leigh M, Ribeiro C, Yankaskas J, Burns K, Gilligan P, et al. (August 2002). "Patterns of epithelial cell invasion by different species of the Burkholderia cepacia complex in well-differentiated human airway epithelia". Infection and Immunity. 70 (8): 4547–4555. doi:10.1128/IAI.70.8.4547-4555.2002. PMC   128168 . PMID   12117967.
  43. ↑ Savoia D, Zucca M (June 2007). "Clinical and environmental Burkholderia strains: biofilm production and intracellular survival". Current Microbiology. 54 (6): 440–444. doi:10.1007/s00284-006-0601-9. PMID   17457645. S2CID   35624527.
  44. ↑ Kjelleberg S, Givskov M (2007). The biofilm mode of life : mechanisms and adaptations. Wymondham: Horizon Bioscience. ISBN   978-1-904933-33-5. OCLC   153550538.
  45. 1 2 Eberl L (April 2006). "Quorum sensing in the genus Burkholderia". International Journal of Medical Microbiology. Quorum sensing in human pathogens. 296 (2–3): 103–110. doi:10.1016/j.ijmm.2006.01.035. PMID   16490397.
  46. 1 2 Courtney JM, Dunbar KE, McDowell A, Moore JE, Warke TJ, Stevenson M, Elborn JS (June 2004). "Clinical outcome of Burkholderia cepacia complex infection in cystic fibrosis adults". Journal of Cystic Fibrosis. 3 (2): 93–98. doi: 10.1016/j.jcf.2004.01.005 . PMID   15463892.
  47. 1 2 Anselmo MA, Lands LC (January 2008). "Chapter 60 - Overview". In Taussig LM, Landau LI (eds.). Pediatric Respiratory Medicine (Second ed.). Philadelphia: Mosby. pp. 845–857. doi:10.1016/b978-032304048-8.50064-5. ISBN   978-0-323-04048-8.
  48. ↑ Jones AM, Dodd ME, Govan JR, Barcus V, Doherty CJ, Morris J, Webb AK (November 2004). "Burkholderia cenocepacia and Burkholderia multivorans: influence on survival in cystic fibrosis". Thorax. 59 (11): 948–951. doi:10.1136/thx.2003.017210. PMC   1746874 . PMID   15516469.
  49. 1 2 3 Bibi F, Ilyas N, Arshad M, Khalid A, Saeed M, Ansar S, Batley J (March 2022). "Formulation and efficacy testing of bio-organic fertilizer produced through solid-state fermentation of agro-waste by Burkholderia cenocepacia". Chemosphere. 291 (Pt 3): 132762. doi:10.1016/j.chemosphere.2021.132762. PMID   34740700. S2CID   242072559.
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