|Alma mater||University of Wisconsin-Madison|
|Known for||Work in the areas of helicases and single stranded DNA binding proteins|
|Awards||Marvin A. Brennecke Professor of Biological Chemistry|
|Fields||Biophysics, Physical Chemistry|
|Doctoral advisor||[Tom Record]|
Timothy M. Lohman (born 1951) earned his Ph.D. in physical chemistry from the University of Wisconsin-Madison in 1977.After completing his Ph.D., he furthered his training with postdoctoral research at the University of California and the University of Oregon. He is currently a professor in the department of biochemistry and molecular biophysics at the Washington University School of Medicine. He has been named to the position of Marvin A. Brennecke Professor of Biological Chemistry and in 2008 served as president of the Gibbs Society of Biological Thermodynamics. He will be giving the second annual Gary K. Ackers Lecture at the 24th annual meeting of the Gibbs Society of Biological Thermodynamics.
Lohman's research has centered on obtaining a molecular understanding of the mechanisms of protein-nucleic acid interactions involved in DNA metabolism, in particular, DNA motor proteins, such as helicases and translocases, and single stranded DNA binding proteins. thermodynamic, kinetic, structural and single molecule approaches are used by his lab to observe these interactions.
Dr. Lohman is married and has two children. Professor Lohman is an accomplished golfer (USGA handicap index 13.1, as of July 2012).
Nucleic acids are biopolymers, macromolecules, essential to all known forms of life. They are composed of nucleotides, which are the monomer components: a 5-carbon sugar, a phosphate group and a nitrogenous base. The two main classes of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). If the sugar is ribose, the polymer is RNA; if the sugar is deoxyribose, a version of ribose, the polymer is DNA.
Helicases are a class of enzymes thought to be vital to all organisms. Their main function is to unpack an organism's genetic material. Helicases are motor proteins that move directionally along a nucleic acid phosphodiester backbone, separating two hybridized nucleic acid strands, using energy from ATP hydrolysis. There are many helicases, representing the great variety of processes in which strand separation must be catalyzed. Approximately 1% of eukaryotic genes code for helicases.
Okazaki fragments are short sequences of DNA nucleotides which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication. They were discovered in the 1960s by the Japanese molecular biologists Reiji and Tsuneko Okazaki, along with the help of some of their colleagues.
In molecular biology, a termination factor is a protein that mediates the termination of RNA transcription by recognizing a transcription terminator and causing the release of the newly made mRNA. This is part of the process that regulates the transcription of RNA to preserve gene expression integrity and are present in both eukaryotes and prokaryotes, although the process in bacteria is more widely understood. The most extensively studied and detailed transcriptional termination factor is the Rho (ρ) protein of E. coli.
A nick is a discontinuity in a double stranded DNA molecule where there is no phosphodiester bond between adjacent nucleotides of one strand typically through damage or enzyme action. Nicks allow DNA strands to untwist during replication, and are also thought to play a role in the DNA mismatch repair mechanisms that fix errors on both the leading and lagging daughter strands.
Adenine nucleotide translocator (ANT), also known as the ADP/ATP translocase (ANT), ADP/ATP carrier protein (AAC) or mitochondrial ADP/ATP carrier, exchanges free ATP with free ADP across the inner mitochondrial membrane. ANT is the most abundant protein in the inner mitochondrial membrane and belongs to mitochondrial carrier family.
The minichromosome maintenance protein complex (MCM) is a DNA helicase essential for genomic DNA replication. Eukaryotic MCM consists of six gene products, Mcm2–7, which form a heterohexamer. As a critical protein for cell division, MCM is also the target of various checkpoint pathways, such as the S-phase entry and S-phase arrest checkpoints. Both the loading and activation of MCM helicase are strictly regulated and are coupled to cell growth cycles. Deregulation of MCM function has been linked to genomic instability and a variety of carcinomas.
Virginia Zakian is the Harry C. Wiess Professor in the Life Sciences in the Department of Molecular Biology at Princeton University. She is the director of the Zakian Lab, which has done important research in topics such as telomere-binding protein, telomere recombination, and telomere position effects, at Princeton University. She is a fellow at the American Academy of Microbiology and the American Association for the Advancement of Science., and is an elected member of the National Academy of Sciences (2018). Zakian served as the chair of "Princeton's Task force on the Status of Women Faculty in the Natural Sciences and Engineering at Princeton" from 2001-2003, in 2003 Zakian became Princeton University's representative to Nine Universities, Gender Equity Analysis She was elected as a member of the American Academy of Arts and Sciences in 2019.
Danny Ray Welch is an American Cancer Biologist and founding director of the University of Kansas Medical Center's Department of Cancer Biology. Welch is also a professor at the University of Kansas School of Medicine and director of the NFCR Center for Metastasis Research at KU. His research is in the area of Metastasis Suppressor Genes and the biology of metastasis.
Transoral laser microsurgery is a form of minimally invasive surgery used by several medical centers, including the Advent Health Cancer Institute, Orlando Florida and Mayo Clinic to remove small and medium tumors through the mouth. It's selectively used for larger tumors. Transoral laser microsurgery allows surgeons to remove tumors from the voice box with no external incisions and is especially applied to HPV-mediated oropharynx malignancy It also allows access to tumors that are not reachable with robotic surgery and is significantly conserving of normal tissue.
Gordon G. Hammes is a distinguished service professor of biochemistry, emeritus, at Duke University, professor emeritus at Cornell University, and member of United States National Academy of Sciences. Hammes' research involves the study of enzyme mechanisms and enzyme regulation.
Stephen Charles Kowalczykowski is a Distinguished Professor of Microbiology and Molecular Genetics at the University of California at Davis. His research focuses on the biochemistry and molecular biology of DNA repair and homologous recombination. His lab combines fluorescence microscopy, optical trapping and microfluidics to manipulate and visualize single molecules of DNA and the enzymes involved in processing and repairing DNA. He calls this scientific approach, "visual biochemistry". Stephen Kowalczykowski was elected to the American Society for Arts and Science in 2005, the National Academy of Sciences in 2007 and was a Harvey Society Lecturer at Rockefeller University in 2012.
ZGRF1 is a protein in humans that is encoded by the ZGRF1 gene that has a weight of 236.6 kDa. The ZGRF1 gene product localizes to the cell nucleus and promotes DNA repair by stimulating homologous recombination. This gene shows relatively low expression in most human tissues, with increased expression in situations of chemical dependence. ZGRF1 is orthologous to nearly all kingdoms of Eukarya. Functional domains of this protein link it to a series of helicases, most notably the AAA_12 and AAA_11 domains.
Rommie E. Amaro is a professor and endowed chair of chemistry and biochemistry and the director of the National Biomedical Computation Resource at the University of California, San Diego. Her research focuses on development of computational methods in biophysics for applications to drug discovery.
James Michael Berger is an American academic working as a professor of biophysics and biophysical chemistry at Johns Hopkins University School of Medicine, where he is also the co-director of the Cancer Chemical and Structural Biology Program at the Sidney Kimmel Comprehensive Cancer Center and the director of the Johns Hopkins Institute for Basic Biomedical Sciences. His main area of research is the functions of molecular cellular machinery.
Rohit Pappu is an Indian-born computational and theoretical biophysicist. He is the Gene K. Beare Distinguished Professor of Engineering and the director of the Center for Science & Engineering of Living Systems (CSELS) at Washington University in St. Louis.
Linda Randall is a Professor Emerita of Biochemistry and Wurdack Chair Emerita of Biological Chemistry at the University of Missouri. Her research has shown unexpected and complex details of the movement of newly made proteins from the cytosol across membranes into the organelles of the cell. In particular, she found that the entire protein was kept unfolded by association with a chaperone and not just directed to cross membranes by its terminal leader sequence. In 1997, she was elected to the National Academy of Sciences of the USA because of the excellence of this work. She has received a number of other honors and awards.
Charles Clifton Richardson is an American biochemist and professor at Harvard University. Richardson received his undergraduate education at Duke University, where he majored in medicine. He received his M.D. at Duke Medical School in 1960. Richardson works as a professor at Harvard Medical School, and he served as editor/associate editor of the Annual Review of Biochemistry from 1972 to 2003. Richardson received the American Chemical Society Award in Biological Chemistry in 1968, as well as numerous other accolades.
Karen Renee Gibson Fleming is a Professor of Biophysics at Johns Hopkins University. She investigates the energetics of transmembrane helix-helix interactions. Fleming was awarded the 2020 Protein Society Carl Brändén Award.
Lynette Cegelski is an American physical chemist and chemical biologist who studies extracellular structures such as biofilms and membrane proteins. She is an associate professor of chemistry and, by courtesy, of chemical engineering at Stanford University. She is a Stanford Bio-X and Stanford ChEM-H affiliated faculty member.