Transmembrane protein 217

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Transmembrane Protein 217 is a protein encoded by the gene TMEM217. TMEM217 has been found to have expression correlated with the lymphatic system and endothelial tissues and has been predicted to have a function linked to the cytoskeleton. [1] [2] [3]

Contents

Gene

TMEM217 is located on the chromosome 6 minus strand at 6p21.2. [4] The gene consists of 46,857 base pairs and is flanked by TBC1D22B (TBC1 Domain Family Member 22B) and PIM1. [4] [5] It was previously known as C6orf128 (Chromosome 6 open reading frame 128). [4]

mRNA

TMEM217 has three common isoforms formed from the alternative splicing of three exons. Isoform 1 translates for the longest polypeptide, consisting of 1590 nucleotides. [6] The 5’ un-translated region of isoform 1 is relatively short and is predicted to fold into several stem loop domains within conserved areas of the un-translated region. [7]

Protein

Primary Protein Sequence

The longest polypeptide of transmembrane protein 217 consists of 229 amino acids. [8] This protein isoform has a predicted weight of 26.6 kDa and isoelectric point at a pH of 9.3. [9] [10] It is notably rich in isoleucine and phenylalanine, and deficient in alanine, aspartate, and proline compared to other proteins. [11] Transmembrane protein 217 contains the domain of unknown function, DUF4534, between amino acids 11-171. [12]

Secondary Structure

Transmembrane protein 217 is predicted to have four transmembrane domains. [8] [13] These transmembrane domains consist primarily of uncharged amino acids in predicted alpha helices. [11] [14] The N-terminus and C-terminus of the protein are predicted to be facing the cytosol with the C-terminus containing a long predicted coiled tail extending from the final transmembrane domain. [13]

Post-Translational Modifications

There are several predicted phosphorylation and glycosylation sites on transmembrane protein 217 in highly conserved parts of the protein, where the phosphorylation sites are located primarily on the C-terminal tail. [15] [16] [17] There are also two highly conserved cysteine residues, which have the potential to form a disulfide bond in the extracellular space between the first and second transmembrane domains.

The image depicts a conceptual diagram of transmembrane protein 217. The protein is given from N-terminus to C-terminus with predicted cellular, transmembrane and extracellular domains present (colored blue, grey, and orange respectively). The most highly conserved predicted post-translational modifications are provided as well. The following predicted post-translational modifications are provided: phosphorylation sites, disulfide bond, and a C-linked mannosylation. Transmembrane 217 Conceptual Protein.svg
The image depicts a conceptual diagram of transmembrane protein 217. The protein is given from N-terminus to C-terminus with predicted cellular, transmembrane and extracellular domains present (colored blue, grey, and orange respectively). The most highly conserved predicted post-translational modifications are provided as well. The following predicted post-translational modifications are provided: phosphorylation sites, disulfide bond, and a C-linked mannosylation.

Expression

TMEM217 is not ubiquitously expressed. The gene tends to have expression correlated to lymphatic system, vascular/arterial endothelial tissue, and notable expression in the bladder based on expression profiles and microarray analysis. [1] [2] Other tissues that have been shown to express TMEM217 include: connective tissues, the liver, mammary glands, the testis, and the cervix. [1] Co-expression analyses have found that TMEM217 was up-regulated in response to mechanical stretch in dermal fibroblast cells and in response to the resveratrol derivative, DMU-212, in vascular endothelial tissues. [3] [18]

Function

No known function has been attributed to TMEM217, however a co-expression analysis in dermal fibroblasts has predicted the protein to have a potential association with the cytoskeleton. [3]

Clinical Significance

Single nucleotide polymorphisms in TMEM217 have been linked to Alzheimer’s disease and diabetic retinopathy. [19] [20] TMEM217 was also found to have similar expression patterns as TRPM2, a biomarker linked to breast carcinoma. [21] Expression profiles have also linked elevated TMEM217 expression to bladder cancer and lymphoma. [1]

Homology

TMEM217 was found to have orthologs in organisms as early as the scaled fish, which diverged 420 million years ago. [22] Although found in organisms as early as fish and reptiles, TMEM217 has no known orthologs in any bird species. [23] [24]

TMEM217 has no known paralogs.

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References

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