Biosafety is the prevention of large-scale loss of biological integrity, focusing both on ecology and human health. These prevention mechanisms include conduction of regular reviews of the biosafety in laboratory settings, as well as strict guidelines to follow. Biosafety is used to protect from harmful incidents. Many laboratories handling biohazards employ an ongoing risk management assessment and enforcement process for biosafety. Failures to follow such protocols can lead to increased risk of exposure to biohazards or pathogens. Human error and poor technique contribute to unnecessary exposure and compromise the best safeguards set into place for protection.
Personal protective equipment (PPE) is protective clothing, helmets, goggles, or other garments or equipment designed to protect the wearer's body from injury or infection. The hazards addressed by protective equipment include physical, electrical, heat, chemicals, biohazards, and airborne particulate matter. Protective equipment may be worn for job-related occupational safety and health purposes, as well as for sports and other recreational activities. "Protective clothing" is applied to traditional categories of clothing, and "protective gear" applies to items such as pads, guards, shields, or masks, and others.
A biosafety level (BSL) is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility. The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4). In the United States, the Centers for Disease Control and Prevention (CDC) have specified these levels. In the European Union, the same biosafety levels are defined in a directive. In Canada the four levels are known as Containment Levels. Facilities with these designations are also sometimes given as P1 through P4, as in the term "P3 laboratory".
A laboratory is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurement may be performed. Laboratory services are provided in a variety of settings: physicians offices, clinics, hospitals, and regional and national referral centers.
A fume hood is a type of local ventilation device that is designed to limit exposure to hazardous or toxic fumes, vapors or dusts.
A cleanroom or clean room is a facility ordinarily utilized as a part of specialized industrial production or scientific research, including the manufacture of pharmaceutical items, integrated circuits, CRT, LCD, OLED and microLED displays. Cleanrooms are designed to maintain extremely low levels of particulates, such as dust, airborne organisms, or vaporized particles. Cleanrooms typically have a cleanliness level quantified by the number of particles per cubic meter at a predetermined molecule measure. The ambient outdoor air in a typical urban area contains 35,000,000 particles for each cubic meter in the size range 0.5 μm and bigger in measurement, equivalent to an ISO 9 cleanroom, while by comparison an ISO 1 cleanroom permits no particles in that size range and just 12 particles for each cubic meter of 0.3 μm and smaller.
Ventilation is the intentional introduction of outdoor air into a space. Ventilation is mainly used to control indoor air quality by diluting and displacing indoor pollutants; it can also be used to control indoor temperature, humidity, and air motion to benefit thermal comfort, satisfaction with other aspects of indoor environment, or other objectives.
A dry cell is a type of electric battery, commonly used for portable electrical devices. It was developed in 1886 by the German scientist Carl Gassner, after development of wet zinc-carbon batteries by Georges Leclanché in 1866. The modern version was developed by Japanese Yai Sakizo in 1887.
An electronic lab notebook is a computer program designed to replace paper laboratory notebooks. Lab notebooks in general are used by scientists, engineers, and technicians to document research, experiments, and procedures performed in a laboratory. A lab notebook is often maintained to be a legal document and may be used in a court of law as evidence. Similar to an inventor's notebook, the lab notebook is also often referred to in patent prosecution and intellectual property litigation.
Air showers are specialized enclosed antechambers which are incorporated as entryways of cleanrooms and other controlled environments to reduce particle contamination. Air showers utilize high-pressure, HEPA- or ULPA-filtered air to remove dust, fibrous lint and other contaminants from personnel or object surfaces. The forceful "cleansing" of surfaces prior to entering clean environments reduces the number of airborne particulates introduced.
An eye dropper, also known as a Pasteur pipette, or dropper, is a device used to transfer small quantities of liquids. They are used in the laboratory and also to dispense small amounts of liquid medicines. A very common use was to dispense eye drops into the eye. The commonly recognized form is a glass tube tapered to a narrow point and fitted with a rubber bulb at the top, although many styles of both plastic and glass droppers exist. The combination of the pipette and rubber bulb has also been referred to as a teat pipette. The Pasteur pipette name is from the French scientist Louis Pasteur, who used a variant of them extensively during his research. In the past, there was no equipment to transfer a chemical solution without exposing it to the external environment. The hygiene and purity of chemical compounds is necessary for the expected result of each experiment. The eye dropper, both glass and plastic types, can be sterilized and plugged with a rubber bulb at the open end of the pipette preventing any contamination from the atmosphere. Generally, they are considered cheap enough to be disposable, however, so long as the glass point is not chipped, the eye dropper may be washed and reused indefinitely.
In natural and social science research, a protocol is most commonly a predefined procedural method in the design and implementation of an experiment. Protocols are written—or in some cases electronically recorded—whenever it is desirable to standardize a laboratory method to ensure successful replication of results by others in the same laboratory or by other laboratories. Additionally, and by extension, protocols have the advantage of facilitating the assessment of experimental results through peer review. In addition to detailed procedures, equipment, and instruments, protocols will also contain study objectives, reasoning for experimental design, reasoning for chosen sample sizes, safety precautions, and how results were calculated and reported, including statistical analysis and any rules for predefining and documenting excluded data to avoid bias.
The Pedestrian Accessibility and Movement Environment Laboratory (PAMELA) is a research facility located at University College London in the United Kingdom. It's designed to study human interactions in controlled conditions by replicating real-world environments such as urban streets and public parks. The laboratory has an 80-square-metre (860 sq ft) artificial pavement platform which is used to simulate everyday scenarios, from different types of pedestrians to varying pavement conditions. Its experiments are intended to create safer streets and more user-friendly public spaces.
Mold or mould, also sometimes referred to as mildew, is a fungal growth that develops on wet materials. Mold is a natural part of the environment and plays an important part in nature by breaking down dead organic matter such as fallen leaves and dead trees; indoors, mold growth should be avoided. Mold reproduce by means of tiny spores. The spores are like seeds, but invisible to the naked eye, that float through the air and deposit on surfaces. When the temperature, moisture, and available nutrient conditions are correct, the spores can form into new mold colonies where they are deposited. There are many types of mold, but all require moisture and a food source for growth.
A biosafety cabinet (BSC)—also called a biological safety cabinet or microbiological safety cabinet—is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with pathogens requiring a defined biosafety level. Several different types of BSC exist, differentiated by the degree of biocontainment required. BSCs first became commercially available in 1950.
Underfloor air distribution (UFAD) is an air distribution strategy for providing ventilation and space conditioning in buildings as part of the design of a HVAC system. UFAD systems use an underfloor supply plenum located between the structural concrete slab and a raised floor system to supply conditioned air through floor diffusers directly into the occupied zone of the building. UFAD systems are similar to conventional overhead systems (OH) in terms of the types of equipment used at the cooling and heating plants and primary air-handling units (AHU). Key differences include the use of an underfloor air supply plenum, warmer supply air temperatures, localized air distribution and thermal stratification. Thermal stratification is one of the featured characteristics of UFAD systems, which allows higher thermostat setpoints compared to the traditional overhead systems (OH). UFAD cooling load profile is different from a traditional OH system due to the impact of raised floor, particularly UFAD may have a higher peak cooling load than that of OH systems. This is because heat is gained from building penetrations and gaps within the structure itself. UFAD has several potential advantages over traditional overhead systems, including layout flexibility, improved thermal comfort and ventilation efficiency, reduced energy use in suitable climates and life-cycle costs. UFAD is often used in office buildings, particularly highly-reconfigurable and open plan offices where raised floors are desirable for cable management. UFAD is appropriate for a number of different building types including commercials, schools, churches, airports, museums, libraries etc. Notable buildings using UFAD system in North America include The New York Times Building, Bank of America Tower and San Francisco Federal Building. Careful considerations need to be made in the construction phase of UFAD systems to ensure a well-sealed plenum to avoid air leakage in UFAD supply plenums.
A dedicated outdoor air system (DOAS) is a type of heating, ventilation and air-conditioning (HVAC) system that consists of two parallel systems: a dedicated system for delivering outdoor air ventilation that handles both the latent and sensible loads of conditioning the ventilation air, and a parallel system to handle the loads generated by indoor/process sources and those that pass through the building enclosure.
Positive pressure personnel suits (PPPS) — or positive pressure protective suits, informally known as "space suits", "moon suits", "blue suits", etc. — are highly specialized, totally encapsulating, industrial protection garments worn only within special biocontainment or maximum containment (BSL-4) laboratory facilities. These facilities research dangerous pathogens which are highly infectious and may have no treatments or vaccines available. These facilities also feature other special equipment and procedures such as airlock entry, quick-drench disinfectant showers, special waste disposal systems, and shower exits.
Chemical storage is the storage of controlled chemicals or hazardous materials in chemical stores, chemical storage cabinets, or similar devices.
Engineering controls are strategies designed to protect workers from hazardous conditions by placing a barrier between the worker and the hazard or by removing a hazardous substance through air ventilation. Engineering controls involve a physical change to the workplace itself, rather than relying on workers' behavior or requiring workers to wear protective clothing.
