A wet lab, or experimental lab, is a type of laboratory where it is necessary to handle various types of chemicals and potential "wet" hazards, so the room has to be carefully designed, constructed, and controlled to avoid spillage and contamination.
A dry lab might have large experimental equipment but minimal chemicals, or instruments for analyzing data produced elsewhere. [1]
A wet lab is a type of laboratory in which a wide range of experiments are performed, for example, characterizing of enzymes in biology, titration in chemistry, diffraction of light in physics, etc. - all of which may sometimes involve dealing with hazardous substances. [2] Due to the nature of these experiments, the proper appropriate arrangement of safety equipment are of great importance. [3]
The researchers (the occupants) are required to know basic laboratory techniques including safety procedures and techniques related to the experiments that they perform. [4]
At the present, lab design tends to focus on increasing the interactions between researchers through the use of open plans, allowing the space and opportunity for researchers to exchange ideas, share equipment, and share storage space; increasing productivity and efficiency of experiments. [5] This style of design has been proposed to support team-based work, though more compartmentalised or individual spaces are still important for some types of processes which require separate/isolated space such as electron microscopes, tissue cultures, work/workers that may be disturbed by noise levels, etc. [5]
Flexibility of laboratory design should also be promoted, for example, the wall and ceiling should be removable in case of expansion or contraction, the pipes, tubes and fume hoods should also be removable for future expansion, reallocation and change of use. A well thought-through design will ensure that a lab can be adjusted for any future use. [6] The sustainability of resources is also a concern, so the amount of resources and energy used in the lab should be reduced where possible to save the environment, but still yield the same products. [7]
As a laboratory consists of many areas such as wet lab, dry lab and office areas, wet labs should be separated from other spaces using controlling devices or dividers to prevent cross-contamination or spillage. [8]
Due to the nature of processes used in wet labs, the environmental conditions may need to be carefully considered and controlled using a cleanroom system.
Laboratory glassware is a variety of equipment used in scientific work, traditionally made of glass. Glass may be blown, bent, cut, molded, or formed into many sizes and shapes. It is commonly used in chemistry, biology, and analytical laboratories. Many laboratories have training programs to demonstrate how glassware is used and to alert first–time users to the safety hazards involved with using glassware.
Biosafety is the prevention of large-scale loss of biological integrity, focusing both on ecology and human health. These prevention mechanisms include the conduction of regular reviews of biosafety in laboratory settings, as well as strict guidelines to follow. Biosafety is used to protect from harmful incidents. Many laboratories handling biohazards employ an ongoing risk management assessment and enforcement process for biosafety. Failures to follow such protocols can lead to increased risk of exposure to biohazards or pathogens. Human error and poor technique contribute to unnecessary exposure and compromise the best safeguards set into place for protection.
Personal protective equipment (PPE) is protective clothing, helmets, goggles, or other garments or equipment designed to protect the wearer's body from injury or infection. The hazards addressed by protective equipment include physical, electrical, heat, chemical, biohazards, and airborne particulate matter. Protective equipment may be worn for job-related occupational safety and health purposes, as well as for sports and other recreational activities. Protective clothing is applied to traditional categories of clothing, and protective gear applies to items such as pads, guards, shields, or masks, and others. PPE suits can be similar in appearance to a cleanroom suit.
An experiment is a procedure carried out to support or refute a hypothesis, or determine the efficacy or likelihood of something previously untried. Experiments provide insight into cause-and-effect by demonstrating what outcome occurs when a particular factor is manipulated. Experiments vary greatly in goal and scale but always rely on repeatable procedure and logical analysis of the results. There also exist natural experimental studies.
A laboratory is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurement may be performed. Laboratories are found in a variety of settings such as schools, universities, privately owned research institutions, corporate research and testing facilities, government regulatory and forensic investigation centers, physicians' offices, clinics, hospitals, regional and national referral centers, and even occasionally personal residences.
A cleanroom or clean room is an engineered space that maintains a very low concentration of airborne particulates. It is well isolated, well controlled from contamination, and actively cleansed. Such rooms are commonly needed for scientific research and in industrial production for all nanoscale processes, such as semiconductor manufacturing. A cleanroom is designed to keep everything from dust to airborne organisms or vaporised particles away from it, and so from whatever material is being handled inside it.
Contamination is the presence of a constituent, impurity, or some other undesirable element that renders something unsuitable, unfit or harmful for physical body, natural environment, workplace, etc.
Idaho National Laboratory (INL) is one of the national laboratories of the United States Department of Energy and is managed by the Battelle Energy Alliance. Historically, the lab has been involved with nuclear research, although the laboratory does other research as well. Much of current knowledge about how nuclear reactors behave and misbehave was discovered at what is now Idaho National Laboratory. John Grossenbacher, former INL director, said, "The history of nuclear energy for peaceful application has principally been written in Idaho".
A dry cell is a type of electric battery, commonly used for portable electrical devices. Unlike wet cell batteries, which have a liquid electrolyte, dry cells use an electrolyte in the form of a paste, and are thus less susceptible to leakage.
An electronic lab notebook is a computer program designed to replace paper laboratory notebooks. Lab notebooks in general are used by scientists, engineers, and technicians to document research, experiments, and procedures performed in a laboratory. A lab notebook is often maintained to be a legal document and may be used in a court of law as evidence. Similar to an inventor's notebook, the lab notebook is also often referred to in patent prosecution and intellectual property litigation.
An eye dropper, also called Pasteur pipette or simply dropper, is a device used to transfer small quantities of liquids. They are used in the laboratory and also to dispense small amounts of liquid medicines. A very common use was to dispense eye drops into the eye. The commonly recognized form is a glass tube tapered to a narrow point and fitted with a rubber bulb at the top, although many styles of both plastic and glass droppers exist. The combination of the pipette and rubber bulb has also been referred to as a teat pipette. The Pasteur pipette name is from the French scientist Louis Pasteur, who used a variant of them extensively during his research. In the past, there was no equipment to transfer a chemical solution without exposing it to the external environment. The hygiene and purity of chemical compounds is necessary for the expected result of each experiment. The eye dropper, both glass and plastic types, can be sterilized and plugged with a rubber bulb at the open end of the pipette preventing any contamination from the atmosphere. Generally, they are considered cheap enough to be disposable, however, so long as the glass point is not chipped, the eye dropper may be washed and reused indefinitely.
In natural and social science research, a protocol is most commonly a predefined procedural method in the design and implementation of an experiment. Protocols are written whenever it is desirable to standardize a laboratory method to ensure successful replication of results by others in the same laboratory or by other laboratories. Additionally, and by extension, protocols have the advantage of facilitating the assessment of experimental results through peer review. In addition to detailed procedures, equipment, and instruments, protocols will also contain study objectives, reasoning for experimental design, reasoning for chosen sample sizes, safety precautions, and how results were calculated and reported, including statistical analysis and any rules for predefining and documenting excluded data to avoid bias.
Laboratory ovens are a common piece of equipment that can be found in electronics, materials processing, forensic, and research laboratories. These ovens generally provide pinpoint temperature control and uniform temperatures throughout the heating process. The following applications are some of the common uses for laboratory ovens: annealing, die-bond curing, drying or dehydrating, Polyimide baking, sterilizing, evaporating. Typical sizes are from one cubic foot to 0.9 cubic metres (32 cu ft). Some ovens can reach temperatures that are higher than 300 degrees Celsius. These temperatures are then applied from all sides of the oven to provide constant heat to sample.
A biosafety cabinet (BSC)—also called a biological safety cabinet or microbiological safety cabinet—is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with pathogens requiring a defined biosafety level. Several different types of BSC exist, differentiated by the degree of biocontainment they provide. BSCs first became commercially available in 1950.
Interplanetary contamination refers to biological contamination of a planetary body by a space probe or spacecraft, either deliberate or unintentional.
Many laboratories contain significant risks, and the prevention of laboratory accidents requires great care and constant vigilance. Examples of risk factors include high voltages, high and low pressures and temperatures, corrosive and toxic chemicals and chemical vapours, radiation, fire, explosions, and biohazards including infective organisms and their toxins.
Chemical storage is the storage of controlled substances or hazardous materials in chemical stores, chemical storage cabinets, or similar devices.
A radioactive nanoparticle is a nanoparticle that contains radioactive materials. Radioactive nanoparticles have applications in medical diagnostics, medical imaging, toxicokinetics, and environmental health, and are being investigated for applications in nuclear nanomedicine. Radioactive nanoparticles present special challenges in operational health physics and internal dosimetry that are not present for other substances, although existing radiation protection measures and hazard controls for nanoparticles generally apply.
The High Flux Beam Reactor (HFBR) was a research reactor located at Brookhaven National Laboratory, a United States Department of Energy national laboratory located in Upton, New York, on Long Island, approximately 60 miles east of New York City. A successor to the Brookhaven Graphite Research Reactor, the HFBR operated from 1965 until 1996 and has been partially decommissioned.
A cloud laboratory is a heavily automated, centralized research laboratory where scientists can run an experiment from a computer in a remote location. Cloud laboratories offer the execution of life science research experiments under a cloud computing service model, allowing researchers to retain full control over experimental design. Users create experimental protocols through a high-level API and the experiment is executed in the cloud laboratory, with no need for the user to be involved.