Yves Allegro and Daniele Bracciali are the defending champions but Allegro chose not to participate that year.
Bracciali partnered up with Rubén Ramírez Hidalgo, and they won in the final 6–4, 7–5 against James Cerretani and Jeff Coetzee.
Daniele Bracciali and Potito Starace were the defending champions, but lost in the final to Frederico Gil and Carlos Berlocq by 6–3, 7–6(7–5).
Jamie Delgado and Jamie Murray were the defending champions.
Thomas Fabbiano and Matteo Trevisan won in the final 6–2, 7–5, against Daniele Bracciali and Filippo Volandri.
Jonathan Eysseric and Romain Jouan were the defending champions, but chose not to compete.
Rubén Ramírez Hidalgo and Santiago Ventura won the title, defeating Brian Battistone and Andreas Siljeström 6–4, 7–6(3) in the final.
Gianluca Naso and Walter Trusendi were the defending champions, but Naso chose to not compete this year.
Trusendi partnered with Thomas Fabbiano, but they were eliminated in the quarterfinal by Igor Zelenay and Lovro Zovko.
Daniele Bracciali and Alessandro Motti won in the final 6–4, 6–2, against Amir Hadad and Harel Levy.
The 2010 AON Open Challenger was a professional tennis tournament played on outdoor red clay courts. It was the 8th edition of the tournament which was part of the 2010 ATP Challenger Tour. It took place in Genoa, Italy between 7 and 12 September.
Leonardo Azzaro and Alessandro Motti were the defending champions, but only Motti chose to start this year.
He partnered with Daniele Bracciali, however they lost to Brian and Dann Battistone in the quarterfinals.
Frederico Gil and Ivan Dodig defeated Thiago Alves and Lukáš Rosol 6–1, 6–3 in the final.
Rajeev Ram and Bobby Reynolds were the defending champions, but Reynolds decided to not compete due to injury.
As a result, Ram partnered with David Martin, however they were eliminated by brothers Battistone in the quarterfinals.
Brian and Dann Battistone won in the final match 7–5, 7–6(5), against Treat Conrad Huey and Harsh Mankad.
Philipp Marx and Igor Zelenay were the defending champions; however, Marx decided not to participate.
Zelenay partners up with Daniele Bracciali. They lost in the first round against Brian Battistone and Andreas Siljeström.
British pair Colin Fleming and Jamie Murray defeated Travis Parrott and Filip Polášek 6–2, 3–6, [10–6] in the final.
Ilija Bozoljac and Daniele Bracciali were the defending champions; however, they decided not to compete.
Alessio di Mauro and Simone Vagnozzi won the tournament after defeating Evgeny Korolev and Yuri Schukin 6–4, 6–4 in the final.
Leonardo Tavares and Simone Vagnozzi were the defending champions, but only Tavares chose to play this year.
As a result, he partnered up with Frederico Gil. They lost to Martin Emmrich and Andreas Siljeström in the semifinals.
Scott Lipsky and David Martin were the defending champions but decided not to participate.
Martin Emmrich and Andreas Siljeström won the title, defeating Kenny de Schepper and Édouard Roger-Vasselin in the final.
Dustin Brown and Martin Emmrich were the defending champions but decided not to participate together.
Brown plays alongside Michael Kohlmann, while Emmrich partners up with Andreas Siljeström.
They went on to win the title after defeating James Cerretani and Michal Mertiňák 6–4, 6–4 in the final.
Riccardo Ghedin and Stéphane Robert were the defending champions but decided not to participate.
Pierre-Hugues Herbert and Albano Olivetti defeated Paul Hanley and Jordan Kerr 7–5, 1–6, [10–7] in the final to win the title.
Harri Heliövaara and Denys Molchanov were the defending champions but Heliövaara decided not to participate.
Molchanov played alongside Uladzimir Ignatik.
Andre Begemann and Martin Emmrich won the title by defeating Rameez Junaid and Frank Moser 6–7(2–7), 7–6(7–2), [10–8] in the final.
Andre Begemann and Alexander Kudryavtsev were the defending champions but Kudryavtsev decided not to participate.
Begemann played alongside Martin Emmrich.
James Cerretani and Adil Shamasdin won the title, defeating Tomasz Bednarek and Andreas Siljeström 6–3, 2–6, [10–4] in the final.
Jamie Delgado and Ken Skupski were the defending champions, but decided not to participate together.
Delgado played alongside Andreas Siljeström, but they lost to Philipp Marx and Florin Mergea in the second round, while Skupski partnered with Andreas Beck, but they lost to Dustin Brown and Rameez Junaid in the first round.
Andre Begemann and Martin Emmrich defeated Marx and Mergea 7–6(7–4), 6–3 in the final to win the title.
Martin Emmrich and Christopher Kas were the defending champions, but chose not to participate together. Emmrich played alongside Lukáš Rosol, but lost to Daniele Bracciali and Andrey Golubev in the semifinals. Kas teamed up with Philipp Kohlschreiber, but lost to Henri Kontinen and Jarkko Nieminen in the quarterfinals.
Kontinen and Nieminen won the title, defeating Bracciali and Golubev in the final, 6–1, 6–4.
Illya Marchenko and Sergiy Stakhovsky were the defending tennis doubles champions, but neither player chose to participate.
Andre Begemann and Lukáš Rosol were the defending champions, but they did not participate this year. When Prostějov tournament started, Rosol was still in play in doubles competition at French Open.
Andre Begemann 
Brian Battistone 
Andreas Siljeström 
