Binary Alignment Map

BAM file format

Filename extension	.bam
Developed by	Heng Li Bob Handsaker Alec Wysoker Tim Fennell Jue Ruan Nils Homer Gabor Marth Gonçalo Abecasis Richard M. Durbin 1000 Genomes Project [1]
Type of format	Bioinformatics
Extended from	Tab-separated values
Website	samtools.github.io/hts-specs/

Binary Alignment Map (BAM) is the comprehensive raw data of genome sequencing; ^[2] it consists of the lossless, compressed binary representation of the Sequence Alignment Map-files. ^{[3] [4]}

Schema

BAM is the compressed binary representation of SAM (Sequence Alignment Map), a compact and index-able representation of nucleotide sequence alignments. ^[5] The goal of indexing is to retrieve alignments that overlap a specific location quickly without having to go through all of them. Before indexing, BAM must be sorted by reference ID and then leftmost coordinate. ^[1] BAM is in compressed BGZF format.

The BAM format; image from: https://samtools.github.io/hts-specs/SAMv1.pdf

The structure of BAM files include a header section and an alignment section: ^[6]

• Header—The sample name, sample length, and alignment method are all included in this section. The alignments section contains alignments that are linked to specific information in the header section.
• Alignments—The read name, read sequence, read quality, alignment information, and custom tags are all included in this file. The chromosome, start coordinate, alignment quality, and match descriptor string are all included in the read name.
  • Alignment Section includes the following:
    • Read Group (RG)
    • Barcode Tag (BC)
    • Single-end alignment quality (SM)
    • Paired-end alignment quality (AS)
    • Edit distance tag (NM)
    • Amplicon name tag (XN)

BAM format uses 0-based coordinate system, where as SAM uses 1-based coordinate system. BAM can represent values in the range [−2^31 , 2^32). ^[1]

Tools

To view a list of sequencing and analysis tools that work with SAM/BAM click here.

See also

• FASTQ format
• SAM format
• SAMtools
• CRAM format
• List of file formats for molecular biology
• Compression of Genomic Sequencing Data

External links

• SAM format specification

References

1. "Sequence Alignment/Map Format Specification" (PDF). The SAM/BAM Format Specification Working Group. 3 Jun 2021.
2. "Carl Zimmer's Game of Genomes, Season 1: Episode 3, BAM Reveals All". STAT. 11 July 2016. Retrieved 2016-08-21.
3. Li, Heng (2009-06-08). "The Sequence Alignment/Map format and SAMtools" (PDF). Bioinformatics. 25 (16): 2078–9. doi:10.1093/bioinformatics/btp352. PMC   2723002 . PMID   19505943.
4. "Binary Alignment Map". National Cancer Institute Wiki. Retrieved 2016-08-21.
5. "Genome Browser BAM Track Format". genome.ucsc.edu. Retrieved 2022-05-05.
6. "BAM File Format". support.illumina.com. Retrieved 2022-05-05.