Chromosome 10 open reading frame 35 (c10orf35) is a gene that in humans, encodes for a protein-binding, [1] transmembrane [2] protein. The protein contains the domain of unknown function 4605 (DUF4605) [1] which belongs to the protein family pfam15378. [3] This gene is located at locus 10q22.1. [1]
The physical properties of the c10orf35 protein were analyzed, and the molecular weight was predicted to be 13.2 kdal, [4] and the isoelectric point was predicted to be 11.5. [4] The properties of DUF4605 were also analyzed, and the molecular weight was predicted to be 6.0 kdal. [4] The isoelectric point was predicted to be 6.9, [4] which is significantly less basic than the full protein.
This protein includes a highly conserved region between amino acids 1 and 19 discovered by MSA. [5] DUF 4605 is located between amino acids 62 and 92. [1] The secondary structure of the c10orf35 protein was predicted to consist of a β-sheet between amino acids 3 and 5, [6] an α-helix between 61 and 72, [6] and a transmembrane α-helix between 92 and 112. [7] The serine in position 62 can be phosphorylated to form a phosphoserine group. [7]
The protein of interest has a similar, paralogous domain found in c4orf32, [8] which is conserved in mammals, birds, very few reptiles, and one amphibian. [9]
The protein coded for by c10orf35 has many orthologs found mainly in mammals, reptiles, few amphibians, one fish, and one invertebrate. [10] Based on a multiple sequence alignment between representative orthologs, DUF 4605 and a second, highly conserved region were confirmed. [5]
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The c10orf35 protein has expression over the 75th percentile in the brain, spinal cord, and male reproductive organs. [12] Within the male reproductive system, the RNA is found within the testis and prostate, while the protein is expressed in the epididymis. [13] In the brain, the RNA is found in the cerebral cortex, while the protein is expressed ubiquitously throughout the brain. [13]
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The c10orf35 protein has been experimentally shown by Affinity Capture-MS to interact with 7 other proteins [1] as shown below.
| Interacting Protein Symbol | Definition |
|---|---|
| ATP1B3 | ATPase, Na+/K+ Transporting, Beta 3 Polypeptide |
| CD3E | CD3e molecule, epsilon (CD3-TCR complex) |
| GABRE | Gamma-aminobutyric acid (GABA) A receptor, epsilon |
| HTR3C | 5-hydroxytryptamine (serotonin) receptor 3C, ionotropic |
| LPAR1 | Lysophosphatidic acid receptor 1 |
| SGCD | Sarcoglycan, delta (35kDa dystrophin-associated glycoprotein) |
| ZACN | Zinc activated ligand-gated ion channel |
TSR3, or TSR3 Ribosome Maturation Factor, is a hypothetical human protein found on chromosome 16. Its protein is 312 amino acids long and its cDNA has 1214 base pairs. It was previously designated C16orf42.
Transmembrane protein 53, or TMEM53, is a protein that is encoded on chromosome 1 in humans. It has no paralogs but is predicted to have many orthologs across eukaryotes.
C2CD4D, or C2 calcium-dependent domain-containing protein 4D is a protein product of the human genome. The gene that codes for this protein is found on chromosome 1, from 150,076,963 to 150,079,657. The gene contains 2 exons and encodes 353 amino acids. Synonyms for C2CD4D are "FAM148D" and NP_001129475. C2CD4D contains a conserved metal binding domain that is a known as Protein kinase C conserved region 2, subgroup 1. This motif is known to be a member of the C2 superfamily, which is present in phospholipases, protein kinases C, and synaptotagmins. The amino acid sequence of C2CD4D can be accessed at Prior to any post translational modification, C2CD4D has a molecular weight of 37.6 kdal. Although scientists have not yet determined where C2CD4D functions within the cell, C2CD4D has a predicted isoelectric point of 11.636 which severely limits the places in which it can be effective. In addition, C2CD4D does not contain any predicted transmembrane domains or any predicted signal peptides.
Tetratricopeptide repeat 39A is a human protein encoded by the TTC39A gene. TTC39A is also known as DEME-6, KIAA0452, and c1orf34. The function of TTC39A is currently not well understood. The main feature within tetratricopeptide repeat 39A is the domain of unknown function 3808 (DUF3808), spanning almost the entire protein. KIAA0452 can also be seen as an isoform of TTC39A because of differences in genome sequence, but overlap in DUF domain.
TMEM69, also known as Transmembrane protein 69, is a protein that in humans is encoded by the TMEM69 gene. A notable feature of the protein encoded by TMEM69 is the presence of five transmembrane segments.
Family with Sequence Similarity 203, Member B (FAM203B) is a protein encoded by the FAM203B gene (8q24.3) in humans. While FAM203B is only found in humans and possibly non-human primates, its paralog, FAM203A, is highly conserved. The FAM203B protein contains two conserved domains of unknown function, DUF383 and DUF384, and no transmembrane domains. This protein has no known function yet, although the homolog of FAM203A in Caenorhabditis elegans (Y54H5A.2) is thought to help regulate the actin cytoskeleton.
Coiled-coil domain 47 (CCDC47) is a gene located on human chromosome 17, specifically locus 17q23.3 which encodes for the protein PAT complex subunit CCDC47. The protein itself contains coiled-coil domains, the SEEEED superfamily, a domain of unknown function (DUF1682) and a transmembrane domain. The function of the protein is unknown, but it has been proposed that CCDC47 is involved in calcium ion homeostasis and the endoplasmic reticulum overload response.
KIAA1841 is a gene in humans that encodes a protein known as KIAA1841. KIAA1841 is targeted for the nucleus and it predicted to play a role in regulating transcription.
Family with sequence similarity 63, member A is a protein that, is encoded by the FAM63A gene in humans,. It is located on the minus strand of chromosome 1 at locus 1q21.3.
Transmembrane protein 268 is a protein that in humans is encoded by TMEM268 gene. The protein is a transmembrane protein of 342 amino acids long with eight alternative splice variants. The protein has been identified in organisms from the common fruit fly to primates. To date, there has been no protein expression found in organisms simpler than insects.
GPATCH11 is a protein that in humans is encoded by the G-patch domain containing protein 11 gene. The gene has four transcript variants encoding two functional protein isoforms and is expressed in most human tissues. The protein has been found to interact with several other proteins, including two from a splicing pathway. In addition, GPATCH11 has orthologs in all taxa of the eukarya domain.
FAM210B is a gene that which in Homo sapiens encodes the protein FAM210B. It has been conserved throughout evolutionary history, and is highly expressed in multiple tissues within the human body. FAM210B's primary location is the endoplasmic reticulum.
PRR29 is a protein encoded by the PRR29 gene located in humans on chromosome 17 at 17q23.
PROSER1 is a protein that in humans is encoded by the PROSER1 gene.
Transmembrane Protein 217 is a protein encoded by the gene TMEM217. TMEM217 has been found to have expression correlated with the lymphatic system and endothelial tissues, and has been predicted to have a function linked to the cytoskeleton.
TMEM44 is a protein that in humans is encoded by the TMEM44 gene. DKFZp686O18124 is a synonym of TMEM44.
Chromosome 19 open reading frame 44 is a protein that in humans is encoded by the C19orf44 gene. C19orf44 is an uncharacterized protein with an unknown function in humans. C19orf44 is non-limiting implying that the protein exists in other species besides human. The protein contains one domain of unknown function (DUF) that is highly conserved throughout its orthologs. This protein is most highly expressed in the testis and ovary, but also has significant expression in the thyroid and parathyroid. Other names for this protein include: LOC84167.
Transmembrane protein 169 (TMEM169) in humans is encoded by TMEM169 gene. The aliases of TMEM169 include FLJ34263, DKFZp781L2456, and LOC92691. TMEM169 has the highest expression in the brain, particularly the fetal brain. TMEM169 has homologs mammals, reptiles, amphibians, birds, fish, chordates and invertebrates. The most distantly related homolog of TMEM169 is Anopheles albimanus.
Transmembrane protein 104 (TMEM104) is a protein that in humans is encoded by the TMEM104 gene. The aliases of TMEM104 are FLJ00021 and FLJ20255. Humans have a 163,255 base pair long gene coding sequence, 4703 base pair long mRNA, and 496 amino acid long protein sequence. In Eukaryotes, the TMEM104 gene is conserved.
C12orf54 is a protein in humans that is encoded by the C12orf54 gene.