DDX1

DDX1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4XW3
Identifiers
Aliases	DDX1 , DBP-RB, UKVH5d, DEAD/H-box helicase 1, DEAD-box helicase 1
External IDs	OMIM: 601257 MGI: 2144727 HomoloGene: 3627 GeneCards: DDX1
Gene location (Human) Chr. Chromosome 2 (human) [1] Band 2p24.3 Start 15,591,178 bp [1] End 15,634,346 bp [1]
Gene location (Mouse) Chr. Chromosome 12 (mouse) [2] Band 12|12 A1.1 Start 13,266,974 bp [2] End 13,299,214 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right ventricle deltoid muscle triceps brachii muscle middle temporal gyrus parotid gland islet of Langerhans gastrocnemius muscle tibialis anterior muscle ganglionic eminence quadriceps femoris muscle Top expressed in primitive streak spermatid spermatocyte maxillary prominence triceps brachii muscle temporal muscle hair follicle facial motor nucleus medial ganglionic eminence sternocleidomastoid muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function RNA helicase activity DNA binding nucleotide binding helicase activity poly(A) binding chromatin binding transcription coregulator activity DNA/RNA helicase activity protein binding nucleic acid binding nuclease activity double-stranded RNA binding exonuclease activity hydrolase activity ATP binding RNA binding Cellular component cytoplasm membrane nucleoplasm tRNA-splicing ligase complex cytoplasmic stress granule cleavage body nucleus cytosol nucleolus mitochondrion ribonucleoprotein complex Biological process regulation of transcription, DNA-templated response to exogenous dsRNA mRNA processing response to virus protein localization to cytoplasmic stress granule transcription, DNA-templated multicellular organism development spliceosomal complex assembly DNA duplex unwinding double-strand break repair RNA secondary structure unwinding regulation of translational initiation tRNA splicing, via endonucleolytic cleavage and ligation tRNA processing nucleic acid phosphodiester bond hydrolysis immune system process positive regulation of myeloid dendritic cell cytokine production positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response defense response to virus regulation of nucleic acid-templated transcription Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1653 104721 Ensembl ENSG00000079785 ENSMUSG00000037149 UniProt Q92499 Q91VR5 RefSeq (mRNA) NM_004939 NM_134040 RefSeq (protein) NP_004930 NP_598801 Location (UCSC) Chr 2: 15.59 – 15.63 Mb Chr 12: 13.27 – 13.3 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene. ^{[5] [6]}

Function

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. ^[6]

Interactions

DDX1 has been shown to interact with HNRPK. ^[7] DDX1 has also been tentatively shown to interact with hCLE/C14orf166/RTRAF and HSPC117 in a cap-binding complex that activates mRNA translation. ^[8]

References

1. GRCh38: Ensembl release 89: ENSG00000079785 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000037149 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Schmid SR, Linder P (February 1992). "D-E-A-D protein family of putative RNA helicases". Molecular Microbiology. 6 (3): 283–91. doi: 10.1111/j.1365-2958.1992.tb01470.x . PMID   1552844. S2CID   45727289.
6. "Entrez Gene: DDX1 DEAD (Asp-Glu-Ala-Asp) box polypeptide 1".
7. Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ (October 2002). "An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K" (PDF). The Journal of Biological Chemistry. 277 (43): 40403–9. doi: 10.1074/jbc.M206981200 . PMID   12183465. S2CID   25252628.
8. Cho WJ, Yoon WJ, Moon CH, Cha SJ, Song H, Cho HR, Jang SJ, Chung DK, Jeong CS, Park JW (November 2002). "Molecular cloning of a novel chaperone-like protein induced by rhabdovirus infection with sequence similarity to the bacterial extracellular solute-binding protein family 5". The Journal of Biological Chemistry. 277 (44): 41489–96. doi: 10.1074/jbc.M207104200 . PMID   12183466.

Further reading

• Li L, Li HS, Pauza CD, Bukrinsky M, Zhao RY (2006). "Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions". Cell Research. 15 (11–12): 923–34. doi: 10.1038/sj.cr.7290370 . PMID   16354571.
• Godbout R, Squire J (August 1993). "Amplification of a DEAD box protein gene in retinoblastoma cell lines". Proceedings of the National Academy of Sciences of the United States of America. 90 (16): 7578–82. Bibcode:1993PNAS...90.7578G. doi: 10.1073/pnas.90.16.7578 . PMC   47185 . PMID   7689221.
• Kitajima Y, Yatsuki H, Zhang R, Matsuhashi S, Hori K (March 1994). "A novel human homologue of a dead-box RNA helicase family". Biochemical and Biophysical Research Communications. 199 (2): 748–54. doi:10.1006/bbrc.1994.1292. PMID   8135819.
• Akiyama K, Akao Y, Yokoyama M, Nakagawa Y, Noguchi T, Yagi K, Nishi Y (April 1999). "Expression of two dead box genes (DDX1 and DDX6) is independent of that of MYCN in human neuroblastoma cell lines". Biochemistry and Molecular Biology International. 47 (4): 563–8. doi: 10.1080/15216549900201603 . PMID   10319407. S2CID   45609314.
• Golembowski S, Gellrich S, von Zimmermann M, Rutz S, Lippert S, Audring H, Lorenz P, Sterry W, Jahn S (April 2000). "Clonal evolution in a primary cutaneous follicle center B cell lymphoma revealed by single cell analysis in sequential biopsies". Immunobiology. 201 (5): 631–44. doi:10.1016/s0171-2985(00)80080-7. PMID   10834319.
• Bléoo S, Sun X, Hendzel MJ, Rowe JM, Packer M, Godbout R (October 2001). "Association of human DEAD box protein DDX1 with a cleavage stimulation factor involved in 3'-end processing of pre-MRNA". Molecular Biology of the Cell. 12 (10): 3046–59. doi:10.1091/mbc.12.10.3046. PMC   60154 . PMID   11598190.
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (January 2002). "Directed proteomic analysis of the human nucleolus". Current Biology. 12 (1): 1–11. doi: 10.1016/S0960-9822(01)00650-9 . PMID   11790298. S2CID   14132033.
• Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ (October 2002). "An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K" (PDF). The Journal of Biological Chemistry. 277 (43): 40403–9. doi: 10.1074/jbc.M206981200 . PMID   12183465. S2CID   25252628.
• Fang J, Kubota S, Yang B, Zhou N, Zhang H, Godbout R, Pomerantz RJ (December 2004). "A DEAD box protein facilitates HIV-1 replication as a cellular co-factor of Rev". Virology. 330 (2): 471–80. doi:10.1016/j.virol.2004.09.039. PMID   15567440.
• Krishnan V, Zeichner SL (December 2004). "Alterations in the expression of DEAD-box and other RNA binding proteins during HIV-1 replication". Retrovirology. 1: 42. doi: 10.1186/1742-4690-1-42 . PMC   543576 . PMID   15588285.
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (January 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID   15635413. S2CID   4344740.
• Fang J, Acheampong E, Dave R, Wang F, Mukhtar M, Pomerantz RJ (June 2005). "The RNA helicase DDX1 is involved in restricted HIV-1 Rev function in human astrocytes". Virology. 336 (2): 299–307. doi:10.1016/j.virol.2005.03.017. PMID   15892970.
• Kaneko S, Ohira M, Nakamura Y, Isogai E, Nakagawara A, Kaneko M (March 2007). "Relationship of DDX1 and NAG gene amplification/overexpression to the prognosis of patients with MYCN-amplified neuroblastoma". Journal of Cancer Research and Clinical Oncology. 133 (3): 185–92. doi:10.1007/s00432-006-0156-y. PMID   17028906. S2CID   9295969.
• Tu LC, Yan X, Hood L, Lin B (April 2007). "Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells". Molecular & Cellular Proteomics. 6 (4): 575–88. doi: 10.1074/mcp.M600249-MCP200 . PMID   17220478. S2CID   7203032.