DNA2L

DNA2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 5EAY
Identifiers
Aliases	DNA2 , DNA2L, hDNA replication helicase/nuclease 2
External IDs	OMIM: 601810 MGI: 2443732 HomoloGene: 6124 GeneCards: DNA2
Gene location (Human) Chr. Chromosome 10 (human) [1] Band 10q21.3 Start 68,414,064 bp [1] End 68,472,121 bp [1]
Gene location (Mouse) Chr. Chromosome 10 (mouse) [2] Band 10|10 B4 Start 62,782,805 bp [2] End 62,809,964 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte ganglionic eminence right lobe of liver rectum cancellous bone stromal cell of endometrium thymus bone marrow cells right lung appendix Top expressed in primitive streak abdominal wall otic placode medial ganglionic eminence secondary oocyte somite vas deferens maxillary prominence hair follicle endocardial cushion More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding 4 iron, 4 sulfur cluster binding nucleotide binding helicase activity iron-sulfur cluster binding metal ion binding 5'-flap endonuclease activity DNA helicase activity ATPase activity protein binding catalytic activity 5'-3' DNA helicase activity nuclease activity endonuclease activity site-specific endodeoxyribonuclease activity, specific for altered base hydrolase activity ATP binding single-stranded DNA helicase activity RNA binding Cellular component nucleoplasm gamma DNA polymerase complex mitochondrial nucleoid nucleus mitochondrion cytoplasm Biological process mitotic telomere maintenance via semi-conservative replication mitochondrial DNA repair nucleic acid phosphodiester bond hydrolysis DNA double-strand break processing mitochondrial DNA replication cellular response to DNA damage stimulus DNA replication checkpoint signaling positive regulation of DNA replication metabolism base-excision repair DNA replication, removal of RNA primer DNA replication telomere maintenance DNA duplex unwinding DNA repair G-quadruplex DNA unwinding t-circle formation telomere maintenance via semi-conservative replication DNA replication, Okazaki fragment processing replication fork reversal regulation of signal transduction by p53 class mediator Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1763 327762 Ensembl ENSG00000138346 ENSMUSG00000036875 UniProt P51530 Q6ZQJ5 RefSeq (mRNA) NM_001080449 NM_177372 RefSeq (protein) NP_001073918 NP_796346 Location (UCSC) Chr 10: 68.41 – 68.47 Mb Chr 10: 62.78 – 62.81 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

DNA2-like helicase is an enzyme that in humans is encoded by the DNA2 gene. ^{[5] [6] [7]} Dna2, a homolog of DNA2KL present in budding yeast, possesses both helicase and nuclease activity, with which it helps catalyze early steps in homologous recombination. ^[8]

References

1. GRCh38: Ensembl release 89: ENSG00000138346 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000036875 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Eki T, Okumura K, Shiratori A, Abe M, Nogami M, Taguchi H, Shibata T, Murakami Y, Hanaoka F (Mar 1997). "Assignment of the closest human homologue (DNA2L:KIAA0083) of the yeast Dna2 helicase gene to chromosome band 10q21.3-q22.1". Genomics. 37 (3): 408–10. doi:10.1006/geno.1996.0581. PMID   8938459.
6. Masuda-Sasa T, Polaczek P, Campbell JL (Dec 2006). "Single strand annealing and ATP-independent strand exchange activities of yeast and human DNA2: possible role in Okazaki fragment maturation". J Biol Chem. 281 (50): 38555–64. doi: 10.1074/jbc.M604925200 . PMID   17032657.
7. "Entrez Gene: DNA2L DNA2 DNA replication helicase 2-like (yeast)".
8. Mimitou, EP; Symington, LS (May 2009). "Nucleases and helicases take center stage in homologous recombination". Trends in Biochemical Sciences. 34 (5): 264–272. doi:10.1016/j.tibs.2009.01.010. PMID   19375328.

Further reading

• Masuda-Sasa T, Imamura O, Campbell JL (2006). "Biochemical analysis of human Dna2". Nucleic Acids Res. 34 (6): 1865–75. doi:10.1093/nar/gkl070. PMC   1428797 . PMID   16595800.
• Kim JH, Kim HD, Ryu GH, et al. (2006). "Isolation of human Dna2 endonuclease and characterization of its enzymatic properties". Nucleic Acids Res. 34 (6): 1854–64. doi:10.1093/nar/gkl102. PMC   1428795 . PMID   16595799.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Deloukas P, Earthrowl ME, Grafham DV, et al. (2004). "The DNA sequence and comparative analysis of human chromosome 10". Nature. 429 (6990): 375–81. Bibcode:2004Natur.429..375D. doi: 10.1038/nature02462 . PMID   15164054.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Nagase T, Miyajima N, Tanaka A, et al. (1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (1): 37–43. doi: 10.1093/dnares/2.1.37 . PMID   7788527.