ENTPD2

Last updated
ENTPD2
Identifiers
Aliases ENTPD2 , CD39L1, NTPDase-2, ectonucleoside triphosphate diphosphohydrolase 2
External IDs OMIM: 602012 MGI: 1096863 HomoloGene: 20333 GeneCards: ENTPD2
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001246
NM_203468

NM_009849

RefSeq (protein)

NP_001237
NP_982293

NP_033979

Location (UCSC) Chr 9: 137.05 – 137.05 Mb Chr 2: 25.29 – 25.29 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Ectonucleoside triphosphate diphosphohydrolase 2 is an enzyme that in humans is encoded by the ENTPD2 gene. [5] [6]

Contents

The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein. Alternative splicing of this gene results in multiple transcript variants. [6]

It has been shown, by scientists from the University of Warwick, that E-NTPDase2 stimulates the growth of the eye: by testing the enzyme on tadpoles, the tadpoles were found to develop extra eyes on their body.[ citation needed ]

Embryonic yolk sac development

The mouse embryonic yolk sac, comprising the visceral yolk sac (VYS) and parietal yolk sac (PYS), serves as a materno-fetal exchange system, facilitating the transfer of nutrients and removal of wastes. One study aimed to analyze gene expression in VYS and PYS endodermal cells and identify novel genetic markers from microarray data, with a focus on Apoa4, Lrp2, Fxyd2, Slc34a3, and Entpd2, which showed predominant expression in VYS epithelial cells. The study extracted RNA from VYS, PYS, placenta, liver, and small intestine tissues from C3H/HeSlc strain mice. For ENTPD2 (Entpd2) mRNA analysis, digoxigenin-labeled riboprobes were prepared using a DIG RNA labeling kit. Notably, not every VYS epithelial cell showed expression of Entpd2 and Slc34a3, and Entpd2 was additionally detected in the myometrium. The findings suggest that Apoa4, Lrp2, Fxyd2, Slc34a3, and Entpd2, along with Gkn2 and Pga5, can serve as genetic markers for VYS epithelial cells and PYS cells, respectively. [7]

Neuroinflammation

This study investigated the regulation of the ENTPD2 gene and the production of the NTPDase2 protein in brain cells during neuroinflammatory and neurodegenerative conditions. The research primarily focused on rat primary cortical astrocytes and the OLN93 oligodendroglial cell line. The analysis found that NTPDase2-mRNA was the most abundant among ectonucleotidase transcripts in both cell types. In primary astrocytes, NTPDase2-mRNA significantly exceeded other transcripts. When exposed to inflammatory mediators, including IL-6, IL-1β, TNFα, and IFNγ, for 8 and 24 hours, the expression of Entpd2 (the gene encoding NTPDase2) in primary astrocytes remained unaffected at both mRNA and protein levels. However, ATP and the anti-inflammatory cytokine IL-10 increased the levels of Entpd2 mRNA and protein. These findings provide valuable insights into the regulation of NTPDase2 in neuroinflammatory conditions, specifically highlighting the lack of impact on Entpd2 expression by certain proinflammatory cytokines in primary astrocytes. [8]

Taste cell differentiation

The study focuses on the turnover of taste cells, which are constantly replaced throughout an animal's life. The discovery that the transcription factor Etv1 plays a role in regulating the differentiation of taste cells responsible for sweet, umami, and salty tastes is significant. The study examined the expression of certain genes, including Entpd2, in circumvallate papillae (structures on the tongue containing taste buds) of both wild-type (WT) mice and those with Etv1 deficiency (Etv1C/C mice). There is a potential link between Etv1, a transcription factor discussed in the study, and the regulation or expression of Entpd2 in taste cells. This finding advances our understanding of the molecular mechanisms involved in taste cell homeostasis and provides new insights into the lineage of taste cells. [9]

The SARS-CoV-2 virus, responsible for COVID-19, may directly affect taste receptor cells (TRCs) in the oral cavity. The virus binds to angiotensin-converting enzyme 2 (ACE2) on a subset of TRCs, specifically type II cells in taste buds. Biopsies from COVID-19 patients with taste changes confirmed the presence of replicating virus in these cells. The study uses ENTPD2 (ectonucleoside triphosphate diphosphohydrolase 2) as a marker for type I taste cells. The study's findings suggest that there is no overlap of ACE2 (the receptor for the SARS-CoV-2 virus causing COVID-19) with the probe for the transcript of ENTPD2. This suggests that ACE2 is not expressed in the same cells as ENTPD2 in taste buds, providing information about the distribution of ACE2 in relation to different types of taste cells. This could be crucial in understanding how SARS-CoV-2 interacts with specific cells in the oral cavity and how it might impact taste perception.The disruption of stem cells in taste papillae during infection suggests a potential mechanism for sudden taste changes in COVID-19 patients, indicating the need for further research into the virus's impact on taste bud dynamics during and after infection. [10]

Taste sensitivity

Exploring the function of taste buds, this study examines gene-targeted Entpd2-null mice, globally lacking the NTPDase2 enzyme. The Entpd2-null mice exhibited normal numbers and sizes of taste buds. Luciferin/luciferase assays performed on the circumvallate tissue of these knockout mice revealed heightened levels of extracellular ATP. Electrophysiological recordings from both the chorda tympani and glossopharyngeal taste nerves indicated reduced responses to all taste stimuli in Entpd2-null mice. Notably, the depressions were more pronounced in the glossopharyngeal nerve compared to the chorda tympani nerve, encompassing all taste qualities. Specifically, responses to sweet and umami stimuli were more significantly affected in the chorda tympani. The study proposes that the elevated extracellular ATP levels in Entpd2-knockout mice may desensitize P2X receptors associated with nerve fibers, leading to a dampening of taste responses.

Scientist employed various techniques to investigate how the removal of the Entpd2 gene affects taste epithelia. Initially, they utilized RT-PCR to assess the presence of NTPDase2 mRNA in pooled taste buds from fungiform and circumvallate papillae. The results demonstrated that the genetic deletion of Entpd2 successfully eradicated the expression of NTPDase2 mRNA in taste buds. To confirm the presence of all three types of taste cells in the knockout (KO) mice, RT-PCR was used to examine the expression of specific markers for each cell type. Specifically, they looked for GLAST for type I cells, α-gustducin, transient receptor potential melastatin 5 (TRPM5), and phospholipase C β2 (PLCβ2) for type II cells, and synaptosomal-associated protein 25 (SNAP25) for type III cells. The PCR results indicate the presence of all three cell types in both circumvallate and fungiform taste buds of the KO mice. Furthermore, to confirm that NTPDase2 is the sole functional ectoATPase in taste buds, there was comparison of ectoATPase activity in circumvallate papillae between wild-type (WT) and Entpd2-KO animals. Using two different substances, ADP and ATP, helped distinguish specific staining for ectoATPase, representing NTPDase2 or NTPDase8 (26), from less specific nucleotidases that break down both ADP and ATP. In the case of animals with the usual genetic makeup (WT), there was an observed a concentrated reaction product in taste buds when ATP, not ADP, was the substance used. This highlights the high precision of the ectonucleotidase in taste buds, corresponding to the presence of NTPDase2. Conversely, in animals lacking the Entpd2 gene (Entpd2-KO), there was no detectable ectoATPase activity in taste buds when ATP was used, confirming NTPDase2's significant role in degrading ATP in this system. In both the usual genetic makeup and the Entpd2-KO groups, nerve bundles beneath taste buds showed nucleotidase activity when ADP was the substance used, indicating the existence of a different nucleotidase in and around these nerve bundles.

To assess the impact of removing the Entpd2 gene on taste bud synaptic function, the researchers measured responses to taste stimuli using whole-nerve recordings from the chorda tympani and glossopharyngeal nerves in both normal and Entpd2-null animals. The animals lacking Entpd2 exhibited decreased responses to all taste qualities in both nerves. Findings indicate that the failure to break down ATP and its buildup in the taste tissue of Entpd2-knockout mice leads to reduced responses to all taste qualities.

The central discovery of this study is that the genetic removal of NTPDase2, the sole ectoATPase expressed in taste buds, leads to a decline in neural responses to taste stimuli. Despite the unaffected taste bud numbers and cell types in the knockout, the reduced responsiveness is likely due to the absence of ATP degradation, resulting in elevated tissue levels of ATP. Since ATP activates P2X receptors on gustatory nerve fibers, essential for neurotransmission in the taste system, the genetic deletion of ectoATPase is proposed to disrupt purinergic transmission at this critical synapse. In parallel, the area surrounding inactive taste tissue in Entpd2-null mice exhibits heightened nanomolar ATP concentrations, indicating a connection to the desensitization of P2X3 homomers. This suggests a potential link to the observed decrease in responsiveness. Furthermore, the distinctive presence of P2X2 and P2X3 subunits in different taste nerves may elucidate the specific impact on taste perception. [11]

Related Research Articles

<span class="mw-page-title-main">ATPase</span> Dephosphorylation enzyme

ATPases (EC 3.6.1.3, Adenosine 5'-TriPhosphatase, adenylpyrophosphatase, ATP monophosphatase, triphosphatase, SV40 T-antigen, ATP hydrolase, complex V (mitochondrial electron transport), (Ca2+ + Mg2+)-ATPase, HCO3-ATPase, adenosine triphosphatase) are a class of enzymes that catalyze the decomposition of ATP into ADP and a free phosphate ion or the inverse reaction. This dephosphorylation reaction releases energy, which the enzyme (in most cases) harnesses to drive other chemical reactions that would not otherwise occur. This process is widely used in all known forms of life.

A nucleoside triphosphate is a nucleoside containing a nitrogenous base bound to a 5-carbon sugar, with three phosphate groups bound to the sugar. They are the molecular precursors of both DNA and RNA, which are chains of nucleotides made through the processes of DNA replication and transcription. Nucleoside triphosphates also serve as a source of energy for cellular reactions and are involved in signalling pathways.

<span class="mw-page-title-main">Nucleoside-diphosphate kinase</span> Class of enzymes

Nucleoside-diphosphate kinases are enzymes that catalyze the exchange of terminal phosphate between different nucleoside diphosphates (NDP) and triphosphates (NTP) in a reversible manner to produce nucleotide triphosphates. Many NDP serve as acceptor while NTP are donors of phosphate group. The general reaction via ping-pong mechanism is as follows: XDP + YTP ←→ XTP + YDP. NDPK activities maintain an equilibrium between the concentrations of different nucleoside triphosphates such as, for example, when guanosine triphosphate (GTP) produced in the citric acid (Krebs) cycle is converted to adenosine triphosphate (ATP). Other activities include cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor, endocytosis, and gene expression.

<span class="mw-page-title-main">Angiotensin-converting enzyme 2</span> Exopeptidase enzyme that acts on angiotensin I and II

Angiotensin-converting enzyme 2 (ACE2) is an enzyme that can be found either attached to the membrane of cells (mACE2) in the intestines, kidney, testis, gallbladder, and heart or in a soluble form (sACE2). Both membrane bound and soluble ACE2 are integral parts of the renin–angiotensin–aldosterone system (RAAS) that exists to keep the body's blood pressure in check. mACE2 is cleaved by the enzyme ADAM17 that releases its extracellular domain, creating soluble ACE2 (sACE2). ACE2 enzyme activity opposes the classical arm of the RAAS by lowering blood pressure through catalyzing the hydrolysis of angiotensin II into angiotensin (1–7). Angiotensin (1-7) in turns binds to MasR receptors creating localized vasodilation and hence decreasing blood pressure. This decrease in blood pressure makes the entire process a promising drug target for treating cardiovascular diseases.

Gastric hydrogen potassium ATPase, also known as H+/K+ ATPase, is an enzyme which functions to acidify the stomach. It is a member of the P-type ATPases, also known as E1-E2 ATPases due to its two states.

<span class="mw-page-title-main">Taste receptor</span> Type of cellular receptor that facilitates taste

A taste receptor or tastant is a type of cellular receptor which facilitates the sensation of taste. When food or other substances enter the mouth, molecules interact with saliva and are bound to taste receptors in the oral cavity and other locations. Molecules which give a sensation of taste are considered "sapid".

<span class="mw-page-title-main">Ectonucleotidase</span>

Ectonucleotidases consist of families of nucleotide metabolizing enzymes that are expressed on the plasma membrane and have externally oriented active sites. These enzymes metabolize nucleotides to nucleosides. The contribution of ectonucleotidases in the modulation of purinergic signaling depends on the availability and preference of substrates and on cell and tissue distribution.

<span class="mw-page-title-main">GATA transcription factor</span> Transcription factor

GATA transcription factors are a family of transcription factors characterized by their ability to bind to the DNA sequence "GATA".

<span class="mw-page-title-main">ENTPD1</span> Mammalian protein found in Homo sapiens

Ectonucleoside triphosphate diphosphohydrolase-1 also known as CD39, is a typical cell surface enzyme with a catalytic site on the extracellular face.

<span class="mw-page-title-main">TAS1R2</span> Protein

T1R2 - Taste receptor type 1 member 2 is a protein that in humans is encoded by the TAS1R2 gene.

<span class="mw-page-title-main">TAS1R3</span> Mammalian protein found in Homo sapiens

Taste receptor type 1 member 3 is a protein that in humans is encoded by the TAS1R3 gene. The TAS1R3 gene encodes the human homolog of mouse Sac taste receptor, a major determinant of differences between sweet-sensitive and -insensitive mouse strains in their responsiveness to sucrose, saccharin, and other sweeteners.

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V-type proton ATPase subunit G 2 is an enzyme that in humans is encoded by the ATP6V1G2 gene.

<span class="mw-page-title-main">ENTPD6</span> Protein-coding gene in the species Homo sapiens

Ectonucleoside triphosphate diphosphohydrolase 6 is an enzyme that in humans is encoded by the ENTPD6 gene.

<span class="mw-page-title-main">ENPP3</span> Protein-coding gene in the species Homo sapiens

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 is an enzyme that in humans is encoded by the ENPP3 gene.

<span class="mw-page-title-main">ENTPD5</span> Protein-coding gene in the species Homo sapiens

Ectonucleoside triphosphate diphosphohydrolase 5 is an enzyme that in humans is encoded by the ENTPD5 gene.

<span class="mw-page-title-main">ENTPD3</span> Protein-coding gene in the species Homo sapiens

Ectonucleoside triphosphate diphosphohydrolase 3 is an enzyme that in humans is encoded by the ENTPD3 gene.

<span class="mw-page-title-main">Lingual papillae</span> Structure giving the tongue its characteristic rough texture

Lingual papillae are small structures on the upper surface of the tongue that give it its characteristic rough texture. The four types of papillae on the human tongue have different structures and are accordingly classified as circumvallate, fungiform, filiform, and foliate. All except the filiform papillae are associated with taste buds.

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References

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  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000015085 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Chadwick BP, Frischauf AM (September 1997). "Cloning and mapping of a human and mouse gene with homology to ecto-ATPase genes". Mammalian Genome. 8 (9): 668–672. doi:10.1007/s003359900534. PMID   9271669. S2CID   42644202.
  6. 1 2 "Entrez Gene: ENTPD2 ectonucleoside triphosphate diphosphohydrolase 2".
  7. Yagi S, Shiojiri N (January 2017). "Identification of novel genetic markers for mouse yolk sac cells by using microarray analyses". Placenta. 49: 68–71. doi:10.1016/j.placenta.2016.11.013. PMID   28012457.
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  9. Ohmoto M, Jyotaki M, Yee KK, Matsumoto I (April 2023). "A Transcription Factor Etv1/Er81 Is Involved in the Differentiation of Sweet, Umami, and Sodium Taste Cells". eNeuro. 10 (4): ENEURO.0236–22.2023. doi:10.1523/ENEURO.0236-22.2023. PMC   10131560 . PMID   37045597.
  10. Doyle ME, Appleton A, Liu QR, Yao Q, Mazucanti CH, Egan JM (September 2021). "Human Type II Taste Cells Express Angiotensin-Converting Enzyme 2 and Are Infected by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)". The American Journal of Pathology. 191 (9): 1511–1519. doi:10.1016/j.ajpath.2021.05.010. PMC   8179718 . PMID   34102107.
  11. Vandenbeuch A, Anderson CB, Parnes J, Enjyoji K, Robson SC, Finger TE, Kinnamon SC (September 2013). "Role of the ectonucleotidase NTPDase2 in taste bud function". Proceedings of the National Academy of Sciences of the United States of America. 110 (36): 14789–14794. Bibcode:2013PNAS..11014789V. doi: 10.1073/pnas.1309468110 . PMC   3767538 . PMID   23959882.

Further reading