State Road 50 runs across the center of the U.S. state of Florida through Orlando, with its termini at SR 55 at Weeki Wachee and SR 5 in Titusville.
State Road 15A is part of the Florida State Road System, and a suffixed alternate of State Road 15. Along with its parent route, SR 15A is signed north–south.
State Road 21 (SR 21) runs mostly in a southwest-to-northeast direction in the US state of Florida from McMeekin to Jacksonville. It is also known as Blanding Boulevard for much of its length.
State Road 54 is located in the Tampa Bay Area, north of Tampa itself, and runs from US 19 in Elfers in the west to US 301 in Zephyrhills, in the east. In between it passes through Wesley Chapel, Land o' Lakes, and Elfers.
State Road 16 (SR 16) runs from northwest to southeast between Raiford and St. Augustine. It passes through the towns of Starke and Green Cove Springs in addition to providing access to Camp Blanding. Major roads and/or highways that SR 16 crosses include: US 301, SR 21, US 17, SR 13, Interstate 95 and US 1.
State Road 100 is a 153-mile-long (246 km) east–west highway serving northeast Florida. Its western terminus is at the Georgia-Florida border four miles (6.4 km) north of Avoca, Florida ; its eastern terminus is an intersection with Shore Scenic Highway in Flagler Beach. The portion west of Lake City is only signed as portions of US 41 and US 129, both of which run north–south.
State Road 200 is a major diagonal road in central and northeast Florida. Its southern terminus is at US 41 in Hernando. Its eastern terminus is at SR A1A in Fernandina Beach, at the corner of Atlantic Avenue and Fletcher Avenue.
State Road 40 is a 91.8-mile-long (147.7 km) east–west highway across northern and east-central Florida, running from U.S. Highway 41 (US 41) in Rainbow Lakes Estates eastward through Ocala over the Ocklawaha River and through the heart of the Ocala National Forest to SR A1A in Ormond Beach. Names of the road include Silver Springs Boulevard in Ocala, Fort Brooks Road from Silver Springs through Astor, Butler Road in Astor, and Granada Boulevard in Ormond Beach. Former sections in Ormond Beach are named "Old Tomoka Road" and "Old Tomoka Avenue."
State Road 12 (SR 12) is an east–west route in the Florida Panhandle, running from SR 20 in Bristol to U.S. Route 27 (US 27) in Havana.
State Road 26 is a 62.172-mile-long (100.056 km) east–west route across North Central Florida.
State Road 48 (SR 48) was an east–west state highway that ran from U.S. Highway 41 in Floral City, Florida, to Florida State Road 19 in Howey-in-the-Hills, Florida. In the last years of the route's existence, it was a 2.167 miles (3.487 km) state road in Bushnell, Florida, which ran from Interstate 75 (I-75) at exit 314 to U.S. Highway 301 (US 301). Today, all segments are designated County Road 48 (CR 48), where they are under county maintenance. Segments in Sumter County are part of the Scenic Sumter Heritage Byway.
U.S. Highway 41 (US 41) in the state of Florida is a north–south United States Highway. It runs 479 miles (771 km) from Miami in South Florida northwest to the Georgia state line north of the Lake City area. Within the state, US 41 is paralleled by Interstate 75 (I-75) all the way from Miami to Georgia, and I-75 has largely supplanted US 41 as a major highway.
U.S. Route 301 in Florida runs from the Sarasota-Bradenton-Venice, Florida Metropolitan Statistical Area northeast to the Greater Jacksonville Metropolitan Area. The road is a spur of U.S. Route 1, which it intersects in Callahan.
U.S. Route 441 (US 441) in Florida is a north–south United States Highway. It runs 433 miles (697 km) from Miami in South Florida northwest to the Georgia state line, with the overall route continuing to Tennessee in the Rocky Top area.
State Road 39 is a north–south state highway in eastern Pasco and Hillsborough County, Florida, United States. Between Plant City and Zephyrhills, the road is named Paul S. Buchman Highway. North of Zephyrhills, Florida the road is a secret state designation for U.S. Route 301 until the southern terminus of the concurrency with U.S. Route 98 south of Dade City, Florida.
State Road 18 is a short state road in the U.S. state of Florida. It begins in Worthington Springs in southern Union County and travels eastward for 6.60 miles (10.62 km) into Bradford County until it becomes County Road 18 at Brooker. It also has county road extensions, one of which is between Fort White and Worthington Springs, and the other between Brooker and SR 100 north of Theressa.
State Road 464 is a short state and county road in Marion County, Florida. The road is primarily in southern Ocala, but extends outside of the city limits.
State Road 492 is a short state road in Ocala, Florida. It is an east-west road that runs 3.7 miles (6.0 km), is four lanes wide with provisions for center turn lanes where necessary, and serves as a connecting route between U.S. Route 27 and eastern Ocala, Silver Springs, and the Ocala National Forest.
State Road 235 is a south–north state highway in Alachua County, Florida. It runs from U.S. Route 441 in Alachua to Brooker, and has county-maintained segments in Alachua and Bradford Counties, which were once part of SR 235.
State Road 655 (SR 655) is a 7.125-mile-long (11.467 km) state highway in Polk County, Florida, that runs from U.S. Route 17 and County Road 655 at Eloise just south of Winter Haven city limits to U.S. Route 92 in western Auburndale.
US 301 in Starke 
SR 16 at Kingsley Village 
