Lepidoptera or lepidopterans is an order of winged insects that includes butterflies and moths. About 180,000 species of the Lepidoptera have been described, representing 10% of the total described species of living organisms, making it the second largest insect order with 126 families and 46 superfamilies. and one of the most widespread and widely recognizable insect orders in the world.
Moths are a group of insects that includes all members of the order Lepidoptera that are not butterflies. They were previously classified as suborder Heterocera, but the group is paraphyletic with respect to butterflies and neither subordinate taxon is used in modern classifications. Moths make up the vast majority of the order. There are approximately 160,000 species of moth, many of which have yet to be described. Most species of moth are nocturnal, although there are also crepuscular and diurnal species.
Agricultural biotechnology, also known as agritech, is an area of agricultural science involving the use of scientific tools and techniques, including genetic engineering, molecular markers, molecular diagnostics, vaccines, and tissue culture, to modify living organisms: plants, animals, and microorganisms. Crop biotechnology is one aspect of agricultural biotechnology which has been greatly developed upon in recent times. Desired trait are exported from a particular species of Crop to an entirely different species. These transgene crops possess desirable characteristics in terms of flavor, color of flowers, growth rate, size of harvested products and resistance to diseases and pests.
ENU, also known as N-ethyl-N-nitrosourea (chemical formula C3H7N3O2), is a highly potent mutagen. For a given gene in mice, ENU can induce 1 new mutation in every 700 loci. It is also toxic at high doses.
Mosaicism or genetic mosaicism is a condition in which a multicellular organism possesses more than one genetic line as the result of genetic mutation. This means that various genetic lines resulted from a single fertilized egg. Mosaicism is one of several possible causes of chimerism, wherein a single organism is composed of cells with more than one distinct genotype.
The sterile insect technique (SIT) is a method of biological insect control, whereby overwhelming numbers of sterile insects are released into the wild. The released insects are preferably male, as this is more cost-effective and the females may in some situations cause damage by laying eggs in the crop, or, in the case of mosquitoes, taking blood from humans. The sterile males compete with fertile males to mate with the females. Females that mate with a sterile male produce no offspring, thus reducing the next generation's population. Sterile insects are not self-replicating and, therefore, cannot become established in the environment. Repeated release of sterile males over low population densities can further reduce and in cases of isolation eliminate pest populations, although cost-effective control with dense target populations is subjected to population suppression prior to the release of the sterile males.
Forward genetics is a molecular genetics approach of determining the genetic basis responsible for a phenotype. Forward genetics provides an unbiased approach because it relies heavily on identifying the genes or genetic factors that cause a particular phenotype or trait of interest.
Radioresistance is the level of ionizing radiation that organisms are able to withstand.
The codling moth is a member of the Lepidopteran family Tortricidae. They are major pests to agricultural crops, mainly fruits such as apples and pears, and a codling moth larva is often called an "apple worm". Because the larvae are not able to feed on leaves, they are highly dependent on fruits as a food source and thus have a significant impact on crops. The caterpillars bore into fruit and stop it from growing, which leads to premature ripening. Various means of control, including chemical, biological, and preventive, have been implemented. This moth has a widespread distribution, being found on six continents. Adaptive behavior such as diapause and multiple generations per breeding season have allowed this moth to persist even during years of bad climatic conditions.
A chemosterilant is a chemical compound that causes reproductive sterility in an organism. Chemosterilants are particularly useful in controlling the population of species that are known to cause disease, such as insects, or species that are, in general, economically damaging. The sterility induced by chemosterilants can have temporary or permanent effects. Chemosterilants can be used to target one or both sexes, and it prevents the organism from advancing to be sexually functional. They may be used to control pest populations by sterilizing males. The need for chemosterilants is a direct consequence of the limitations of insecticides. Insecticides are most effective in regions in which there is high vector density in conjunction with endemic transmission, and this may not always be the case. Additionally, the insects themselves will develop a resistance to the insecticide either on the target protein level or through avoidance of the insecticide in what is called a behavioral resistance. If an insect that has been treated with a chemosterilant mates with a fertile insect, no offspring will be produced. The intention is to keep the percent of sterile insects within a population constant, such that with each generation, there will be fewer offspring.
Cytoplasmic male sterility is total or partial male sterility in hermaphrodite organisms, as the result of specific nuclear and mitochondrial interactions. Male sterility is the failure to produce functional anthers, pollen, or male gametes. Such male sterility in hermaphrodite populations leads to gynodioecious populations.
Balancer chromosomes are a type of genetically engineered chromosome used in laboratory biology for the maintenance of recessive lethal mutations within living organisms without interference from natural selection. Since such mutations are viable only in heterozygotes, they cannot be stably maintained through successive generations and therefore continually lead to production of wild-type organisms, which can be prevented by replacing the homologous wild-type chromosome with a balancer. In this capacity, balancers are crucial for genetics research on model organisms such as Drosophila melanogaster, the common fruit fly, for which stocks cannot be archived. They can also be used in forward genetics screens to specifically identify recessive lethal mutations. For that reason, balancers are also used in other model organisms, most notably the nematode worm Caenorhabditis elegans and the mouse.
Habrobracon hebetor is a minute wasp of the family Braconidae that is an ectoparasitoid of several species of moth caterpillars. Well known hosts include the larval stage of Plodia interpunctella, the Indianmeal moth, the late larval stage of the Mediterranean flour moth and the almond moth, and the dried fruit moth. This parasitoid has been used commercially as a way to control pests without using chemical insecticides.
Chloridea virescens, commonly known as the tobacco budworm, is a moth of the family Noctuidae found throughout the eastern and southwestern United States along with parts of Central America and South America.
A genetically modified (GM) insect is an insect that has been genetically modified, either through mutagenesis, or more precise processes of transgenesis, or cisgenesis. Motivations for using GM insects include biological research purposes and genetic pest management. Genetic pest management capitalizes on recent advances in biotechnology and the growing repertoire of sequenced genomes in order to control pest populations, including insects. Insect genomes can be found in genetic databases such as NCBI, and databases more specific to insects such as FlyBase, VectorBase, and BeetleBase. There is an ongoing initiative started in 2011 to sequence the genomes of 5,000 insects and other arthropods called the i5k. Some Lepidoptera have been genetically modified in nature by the wasp bracovirus.
Sterility is the physiological inability to effect sexual reproduction in a living thing, members of whose kind have been produced sexually. Sterility has a wide range of causes. It may be an inherited trait, as in the mule; or it may be acquired from the environment, for example through physical injury or disease, or by exposure to radiation.
Mutation breeding, sometimes referred to as "variation breeding", is the process of exposing seeds to chemicals, radiation, or enzymes in order to generate mutants with desirable traits to be bred with other cultivars. Plants created using mutagenesis are sometimes called mutagenic plants or mutagenic seeds.
Holocentric chromosomes are chromosomes that possess multiple kinetochores along their length rather than the single centromere typical of other chromosomes. They were first described in cytogenetic experiments in 1935. Since this first observation, the term holocentric chromosome has referred to chromosomes that: i) lack the primary constriction corresponding to the centromere observed in monocentric chromosomes; and ii) possess multiple kinetochores dispersed along the entire chromosomal axis, such that microtubules bind to the chromosome along its entire length and move broadside to the pole from the metaphase plate. Holocentric chromosomes are also termed holokinetic, because, during cell division, the sister chromatids move apart in parallel and do not form the classical V-shaped figures typical of monocentric chromosomes.
Johannes A. Jehle is a German scientist for insect virology, and plant protection. The focus of his research is the use of microorganisms and viruses for biological control of insect pests and the development of sustainable methods for plant protection. He heads the Institute for Biological Plant Control of the Julius Kühn-Institut in Darmstadt and is an adjunct Professor at the Technical University Darmstadt. He was President of the Society of Invertebrate Pathology in 2016/2018.