An X-ray microscope uses electromagnetic radiation in the X-ray band to produce magnified images of objects. Since X-rays penetrate most objects, there is no need to specially prepare them for X-ray microscopy observations.
Electron backscatter diffraction (EBSD) is a scanning electron microscope (SEM) technique used to study the crystallographic structure of materials. EBSD is carried out in a scanning electron microscope equipped with an EBSD detector comprising at least a phosphorescent screen, a compact lens and a low-light camera. In this configuration, the SEM incident beam hits the tilted sample. As backscattered electrons leave the sample, they interact with the crystal’s periodic atomic lattice planes and diffract according to Bragg's law at various scattering angles before reaching the phosphor screen forming Kikuchi patterns (EBSPs).
Electron crystallography is a method to determine the arrangement of atoms in solids using a transmission electron microscope (TEM). It can involve the use of high-resolution transmission electron microscopy images, electron diffraction patterns including convergent-beam electron diffraction or combinations of these. It has been successful in determining some bulk structures, and also surface structures. Two related methods are low-energy electron diffraction which has solved the structure of many surfaces, and reflection high-energy electron diffraction which is used to monitor surfaces often during growth.
A scanning transmission electron microscope (STEM) is a type of transmission electron microscope (TEM). Pronunciation is [stɛm] or [ɛsti:i:ɛm]. As with a conventional transmission electron microscope (CTEM), images are formed by electrons passing through a sufficiently thin specimen. However, unlike CTEM, in STEM the electron beam is focused to a fine spot which is then scanned over the sample in a raster illumination system constructed so that the sample is illuminated at each point with the beam parallel to the optical axis. The rastering of the beam across the sample makes STEM suitable for analytical techniques such as Z-contrast annular dark-field imaging, and spectroscopic mapping by energy dispersive X-ray (EDX) spectroscopy, or electron energy loss spectroscopy (EELS). These signals can be obtained simultaneously, allowing direct correlation of images and spectroscopic data.
Electron holography is holography with electron waves. Dennis Gabor invented holography in 1948 when he tried to improve image resolution in electron microscope. The first attempts to perform holography with electron waves were made by Haine and Mulvey in 1952; they recorded holograms of zinc oxide crystals with 60 keV electrons, demonstrating reconstructions with approximately 1 nm resolution. In 1955, G. Möllenstedt and H. Düker invented an electron biprism, thus enabling the recording of electron holograms in off-axis scheme. There are many different possible configurations for electron holography, with more than 20 documented in 1992 by Cowley. Usually, high spatial and temporal coherence of the electron beam are required to perform holographic measurements.
Rafal Edward Dunin-Borkowski is a British experimental physicist. He is currently Director of the Institute for Microstructure Research (PGI-5) and the Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C) in Forschungszentrum Jülich and Professor of Experimental Physics in RWTH Aachen University.
In biophysics and related fields, reduced dimension forms (RDFs) are unique on-off mechanisms for random walks that generate two-state trajectories (see Fig. 1 for an example of a RDF and Fig. 2 for an example of a two-state trajectory). It has been shown that RDFs solve two-state trajectories, since only one RDF can be constructed from the data, where this property does not hold for on-off kinetic schemes, where many kinetic schemes can be constructed from a particular two-state trajectory (even from an ideal on-off trajectory). Two-state time trajectories are very common in measurements in chemistry, physics, and the biophysics of individual molecules (e.g. measurements of protein dynamics and DNA and RNA dynamics, activity of ion channels, enzyme activity, quantum dots ), thus making RDFs an important tool in the analysis of data in these fields.
Paul Anthony Midgley FRS is a Professor of Materials Science in the Department of Materials Science and Metallurgy at the University of Cambridge and a fellow of Peterhouse, Cambridge.
Joachim Frank is a German-American biophysicist at Columbia University and a Nobel laureate. He is regarded as the founder of single-particle cryo-electron microscopy (cryo-EM), for which he shared the Nobel Prize in Chemistry in 2017 with Jacques Dubochet and Richard Henderson. He also made significant contributions to structure and function of the ribosome from bacteria and eukaryotes.
Liquid-phase electron microscopy refers to a class of methods for imaging specimens in liquid with nanometer spatial resolution using electron microscopy. LP-EM overcomes the key limitation of electron microscopy: since the electron optics requires a high vacuum, the sample must be stable in a vacuum environment. Many types of specimens relevant to biology, materials science, chemistry, geology, and physics, however, change their properties when placed in a vacuum.
John Marius Rodenburg is Professor in the Department of Electronic and Electrical Engineering at the University of Sheffield.
There are a variety of technologies available for detecting and recording the images, diffraction patterns, and electron energy loss spectra produced using transmission electron microscopy (TEM).
Toshiki Tajima is a Japanese theoretical plasma physicist known for pioneering the laser wakefield acceleration technique with John M. Dawson in 1979. The technique is used to accelerate particles in a plasma and was experimentally realized in 1994, for which Tajima received several awards such as the Nishina Memorial Prize (2006), the Enrico Fermi Prize (2015), the Robert R. Wilson Prize (2019), the Hannes Alfvén Prize (2019) and the Charles Hard Townes Award (2020).
Helen Sarah Margolis is a British physicist who is a Senior Fellow and Head of Science for Time and Frequency at the National Physical Laboratory. Her research considers the use of optical frequency metrology using femtosecond combs.
Amalia Ioana Coldea is a Romanian quantum physicist who is an Associate Professor at the University of Oxford. She was awarded the 2019 Institute of Physics Brian Pippard Prize and the 2011 EuroMagnet Prize.
4D scanning transmission electron microscopy is a subset of scanning transmission electron microscopy (STEM) which utilizes a pixelated electron detector to capture a convergent beam electron diffraction (CBED) pattern at each scan location. This technique captures a 2 dimensional reciprocal space image associated with each scan point as the beam rasters across a 2 dimensional region in real space, hence the name 4D STEM. Its development was enabled by evolution in STEM detectors and improvements computational power. The technique has applications in visual diffraction imaging, phase orientation and strain mapping, phase contrast analysis, among others.
Janina Maultzsch is a German physicist who is the Chair of Experimental Physics at Friedrich-Alexander-Universität Erlangen-Nürnberg. Her research considers the electronic and optical properties of carbon nanomaterials.
Munira Khalil is an American chemist who is the Leon C. Johnson Professor of Chemistry and Department Chair at the University of Washington.
Angus J Wilkinson is a professor of materials science based at University of Oxford. He is a specialist in micromechanics, electron microscopy and crystal plasticity. He assists in overseeing the MicroMechanics group while focusing on the fundamentals of material deformation. He developed the HR-EBSD method for mapping stress and dislocation density at high spatial resolution used at the micron scale in mechanical testing and micro-cantilevers to extract data on mechanical properties that are relevant to materials engineering.
Angus Ian KirklandFInstP FRSC FRMS is the JEOL Professor of Electron Microscopy at the Department of Materials, University of Oxford. Professor Kirkland specialises in High-resolution transmission electron microscopy and Scanning transmission electron microscopy.
