MGAT1

MGAT1
Identifiers
Aliases	MGAT1 , GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, MGAT, GnTI, mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
External IDs	OMIM: 160995 MGI: 96973 HomoloGene: 1804 GeneCards: MGAT1
Gene location (Human) Chr. Chromosome 5 (human) [1] Band 5q35.3 Start 180,784,782 bp [1] End 180,815,652 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11 B1.2|11 29.27 cM Start 49,244,191 bp [2] End 49,263,030 bp [2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • glycosyltransferase activity • transferase activity • acetylglucosaminyltransferase activity • metal ion binding • alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity • manganese ion binding Cellular component • integral component of membrane • extracellular vesicle • Golgi membrane • Golgi apparatus • extracellular exosome • membrane Biological process • protein glycosylation • in utero embryonic development • UDP-N-acetylglucosamine catabolic process • carbohydrate metabolic process • protein N-linked glycosylation • protein N-linked glycosylation via asparagine Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	4245	17308
Ensembl	ENSG00000131446	ENSMUSG00000020346
UniProt	P26572	P27808
RefSeq (mRNA)	NM_001114617 NM_001114618 NM_001114619 NM_001114620 NM_002406	NM_001110148 NM_001110149 NM_001110150 NM_010794
RefSeq (protein)	NP_001108089 NP_001108090 NP_001108091 NP_001108092 NP_002397 NP_001351306 NP_001351308 NP_001351309 NP_001351310 NP_001351311 NP_001351312 NP_001351313 NP_001351314 NP_001351315 NP_001351316 NP_001351317 NP_001351318 NP_001351319 NP_001351320 NP_001351321 NP_001351322 NP_001351323 NP_001351324	NP_001103618 NP_001103619 NP_001103620 NP_034924
Location (UCSC)	Chr 5: 180.78 – 180.82 Mb	Chr 11: 49.24 – 49.26 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase is an enzyme that in humans is encoded by the MGAT1 gene. ^{[5] [6]}

There are over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. ^[6]

References

1. GRCh38: Ensembl release 89: ENSG00000131446 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020346 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Hull E, Sarkar M, Spruijt MP, Hoppener JW, Dunn R, Schachter H (Jun 1991). "Organization and localization to chromosome 5 of the human UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene". Biochem Biophys Res Commun. 176 (2): 608–15. doi:10.1016/S0006-291X(05)80227-X. PMID   1827260.
6. "Entrez Gene: MGAT1 mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase".

Further reading

• Kumar R, Yang J, Eddy RL, et al. (1992). "Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase I". Glycobiology. 2 (4): 383–93. doi:10.1093/glycob/2.4.383. PMID   1421759.
• Kumar R, Yang J, Larsen RD, Stanley P (1991). "Cloning and expression of N-acetylglucosaminyltransferase I, the medial Golgi transferase that initiates complex N-linked carbohydrate formation". Proc. Natl. Acad. Sci. U.S.A. 87 (24): 9948–52. doi: 10.1073/pnas.87.24.9948 . PMC   55291 . PMID   1702225.
• Tan J, D'Agostaro AF, Bendiak B, et al. (1995). "The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2- N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein". Eur. J. Biochem. 231 (2): 317–28. doi:10.1111/j.1432-1033.1995.tb20703.x. PMID   7635144.
• Yip B, Chen SH, Mulder H, et al. (1997). "Organization of the human beta-1,2-N-acetylglucosaminyltransferase I gene (MGAT1), which controls complex and hybrid N-glycan synthesis". Biochem. J. 321 (2): 465–74. doi:10.1042/bj3210465. PMC   1218092 . PMID   9020882.
• Yen CL, Stone SJ, Cases S, et al. (2002). "Identification of a gene encoding MGAT1, a monoacylglycerol acyltransferase". Proc. Natl. Acad. Sci. U.S.A. 99 (13): 8512–7. Bibcode:2002PNAS...99.8512Y. doi: 10.1073/pnas.132274899 . PMC   124292 . PMID   12077311.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC   442148 . PMID   15231748.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". DNA Res. 12 (2): 117–26. doi: 10.1093/dnares/12.2.117 . PMID   16303743.
• Saribas AS, Johnson K, Liu L, et al. (2007). "Refolding of human beta-1-2 GlcNAc transferase (GnT1) and the role of its unpaired Cys 121". Biochem. Biophys. Res. Commun. 362 (2): 381–6. doi:10.1016/j.bbrc.2007.07.199. PMID   17716624.