NASP (gene)

NASP
Identifiers
Aliases	NASP , FLB7527, PRO1999, HMDRA1, nuclear autoantigenic sperm protein
External IDs	OMIM: 603185 MGI: 1355328 HomoloGene: 133894 GeneCards: NASP
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1p34.1 Start 45,583,846 bp [1] End 45,618,904 bp [1]
Gene location (Mouse) Chr. Chromosome 4 (mouse) [2] Band 4 D1|4 53.24 cM Start 116,458,249 bp [2] End 116,485,138 bp [2]
RNA expression pattern Bgee Top expressed in testicle embryo lung corpus callosum kidney stomach More reference expression data BioGPS More reference expression data
Gene ontology Molecular function GO:0001948 protein binding histone binding GO:0032403 protein-containing complex binding Cellular component cytoplasm nucleus nucleoplasm protein-containing complex Biological process protein transport DNA replication-dependent chromatin assembly DNA replication nucleosome assembly DNA replication-independent chromatin assembly cell population proliferation male gonad development blastocyst development histone exchange response to testosterone G1/S transition of mitotic cell cycle cell cycle CENP-A containing chromatin assembly Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	4678	50927
Ensembl	ENSG00000132780	ENSMUSG00000028693
UniProt	P49321	Q99MD9
RefSeq (mRNA)	NM_001195193 NM_002482 NM_152298 NM_172164	NM_001081475 NM_001284229 NM_016777
RefSeq (protein)	NP_001182122 NP_002473 NP_689511	NP_001074944 NP_001271158 NP_058057
Location (UCSC)	Chr 1: 45.58 – 45.62 Mb	Chr 4: 116.46 – 116.49 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Nuclear autoantigenic sperm protein is a protein that in humans is encoded by the NASP gene. ^{[5] [6]} Multiple isoforms are encoded by transcript variants of this gene.

Function

This gene encodes a histone H1 binding protein that is involved in transporting histones into the nucleus of dividing cells. ^[7] The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. ^[6]

References

1. GRCh38: Ensembl release 89: ENSG00000132780 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000028693 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. O'Rand MG, Richardson RT, Zimmerman LJ, Widgren EE (Dec 1992). "Sequence and localization of human NASP: conservation of a Xenopus histone-binding protein". Dev Biol. 154 (1): 37–44. doi:10.1016/0012-1606(92)90045-I. PMID   1426632.
6. "Entrez Gene: NASP nuclear autoantigenic sperm protein (histone-binding)".
7. Alekseev OM, Widgren EE, Richardson RT, O'Rand MG (January 2005). "Association of NASP with HSP90 in mouse spermatogenic cells: stimulation of ATPase activity and transport of linker histones into nuclei". J. Biol. Chem. 280 (4): 2904–11. doi: 10.1074/jbc.M410397200 . PMID   15533935.

Further reading

• Welch JE, Zimmerman LJ, Joseph DR, O'Rand MG (1991). "Characterization of a sperm-specific nuclear autoantigenic protein. I. Complete sequence and homology with the Xenopus protein, N1/N2". Biol. Reprod. 43 (4): 559–68. doi: 10.1095/biolreprod43.4.559 . PMID   2289010.
• Welch JE, O'Rand MG (1991). "Characterization of a sperm-specific nuclear autoantigenic protein. II. Expression and localization in the testis". Biol. Reprod. 43 (4): 569–78. doi: 10.1095/biolreprod43.4.569 . PMID   2289011.
• Kleinschmidt JA, Dingwall C, Maier G, Franke WW (1987). "Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis". EMBO J. 5 (13): 3547–52. doi:10.1002/j.1460-2075.1986.tb04681.x. PMC   1167392 . PMID   3549279.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Lee YH, O'Rand MG (1993). "Ultrastructural localization of a nuclear autoantigenic sperm protein in spermatogenic cells and spermatozoa". Anat. Rec. 236 (3): 442–8. doi:10.1002/ar.1092360304. PMID   8363049. S2CID   22558475.
• Batova I, O'Rand MG (1996). "Histone-binding domains in a human nuclear autoantigenic sperm protein". Biol. Reprod. 54 (6): 1238–44. doi: 10.1095/biolreprod54.6.1238 . PMID   8724350.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Richardson RT, Batova IN, Widgren EE, et al. (2000). "Characterization of the histone H1-binding protein, NASP, as a cell cycle-regulated somatic protein". J. Biol. Chem. 275 (39): 30378–86. doi: 10.1074/jbc.M003781200 . PMID   10893414.
• Batova IN, Richardson RT, Widgren EE, O'Rand MG (2000). "Analysis of the autoimmune epitopes on human testicular NASP using recombinant and synthetic peptides". Clin. Exp. Immunol. 121 (2): 201–9. doi:10.1046/j.1365-2249.2000.01303.x. PMC   1905703 . PMID   10931132.
• Minami N, Sasaki K, Aizawa A, et al. (2001). "Analysis of gene expression in mouse 2-cell embryos using fluorescein differential display: comparison of culture environments". Biol. Reprod. 64 (1): 30–5. doi: 10.1095/biolreprod64.1.30 . PMID   11133655.
• Richardson RT, Bencic DC, O'Rand MG (2001). "Comparison of mouse and human NASP genes and expression in human transformed and tumor cell lines". Gene. 274 (1–2): 67–75. doi:10.1016/S0378-1119(01)00605-9. PMID   11674998.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Alekseev OM, Bencic DC, Richardson RT, et al. (2003). "Overexpression of the Linker histone-binding protein tNASP affects progression through the cell cycle". J. Biol. Chem. 278 (10): 8846–52. doi: 10.1074/jbc.M210352200 . PMID   12509435.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Silva AP, Chen J, Carraro DM, et al. (2004). "Generation of longer 3' cDNA fragments from massively parallel signature sequencing tags". Nucleic Acids Res. 32 (12): e94. doi:10.1093/nar/gnh095. PMC   484194 . PMID   15247327.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi: 10.1073/pnas.0404720101 . PMC   514446 . PMID   15302935.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Groth A, Ray-Gallet D, Quivy JP, et al. (2005). "Human Asf1 regulates the flow of S phase histones during replicational stress". Mol. Cell. 17 (2): 301–11. doi: 10.1016/j.molcel.2004.12.018 . PMID   15664198.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.