OTUB1

OTUB1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2ZFY, 3VON, 4DDG, 4DDI, 4DHZ, 4I6L, 4LDT
Identifiers
Aliases	OTUB1 , OTB1, OTU1, HSPC263, OTU deubiquitinase, ubiquitin aldehyde binding 1
External IDs	OMIM: 608337; MGI: 2147616; HomoloGene: 134542; GeneCards: OTUB1; OMA:OTUB1 - orthologs
Gene location (Human) Chr. Chromosome 11 (human) [1] Band 11q13.1 Start 63,985,853 bp [1] End 64,001,811 bp [1]
Gene location (Mouse) Chr. Chromosome 19 (mouse) [2] Band 19|19 A Start 7,175,567 bp [2] End 7,183,681 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right frontal lobe prefrontal cortex right hemisphere of cerebellum Brodmann area 10 cingulate gyrus anterior cingulate cortex granulocyte pancreatic ductal cell mucosa of ileum Brodmann area 9 Top expressed in dentate gyrus of hippocampal formation granule cell superior frontal gyrus yolk sac ventricular zone neural layer of retina morula tail of embryo lip primary visual cortex spermatocyte More reference expression data BioGPS More reference expression data
Gene ontology Molecular function cysteine-type peptidase activity NEDD8-specific protease activity thiol-dependent deubiquitinase peptidase activity ubiquitin binding protein binding hydrolase activity ubiquitin protein ligase binding Cellular component cytoplasm extracellular exosome nucleus nucleoplasm cytosol Biological process adaptive immune response protein K48-linked deubiquitination immune system process proteolysis cellular response to DNA damage stimulus negative regulation of double-strand break repair negative regulation of histone H2A K63-linked ubiquitination cellular response to interleukin-1 DNA repair protein deubiquitination Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 55611 107260 Ensembl ENSG00000167770 ENSMUSG00000024767 UniProt Q96FW1 Q7TQI3 RefSeq (mRNA) NM_017670 NM_134150 RefSeq (protein) NP_060140 NP_598911 Location (UCSC) Chr 11: 63.99 – 64 Mb Chr 19: 7.18 – 7.18 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Ubiquitin thioesterase OTUB1 also known as otubain-1 is an enzyme that in humans is encoded by the OTUB1 gene. ^{[5] [6]} Alternative splicing results in multiple transcript variants.

Function

Otubain-1 is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains, being specific for lysine⁴⁸ -linked polyubiquitin but not lysine⁶³ -linked polyubiquitin. ^[7] It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. ^[6]

Interactions

OTUB1 has been shown to interact with RNF128 ^[8] and GNB2L1. ^[7]

References

1. GRCh38: Ensembl release 89: ENSG00000167770 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000024767 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Balakirev MY, Tcherniuk SO, Jaquinod M, Chroboczek J (Apr 2003). "Otubains: a new family of cysteine proteases in the ubiquitin pathway". EMBO Rep. 4 (5): 517–22. doi:10.1038/sj.embor.embor824. PMC   1319179 . PMID   12704427.
6. "Entrez Gene: OTUB1 OTU domain, ubiquitin aldehyde binding 1".
7. Edelmann MJ, Iphöfer A, Akutsu M, Altun M, di Gleria K, Kramer HB, Fiebiger E, Dhe-Paganon S, Kessler BM (March 2009). "Structural basis and specificity of human otubain 1-mediated deubiquitination" (PDF). Biochem. J. 418 (2): 379–90. doi:10.1042/BJ20081318. PMID   18954305.
8. Soares L, Seroogy C, Skrenta H, Anandasabapathy N, Lovelace P, Chung CD, Engleman E, Fathman CG (January 2004). "Two isoforms of otubain 1 regulate T cell anergy via GRAIL". Nat. Immunol. 5 (1): 45–54. doi:10.1038/ni1017. PMID   14661020. S2CID   27005972.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Makarova KS, Aravind L, Koonin EV (2000). "A novel superfamily of predicted cysteine proteases from eukaryotes, viruses and Chlamydia pneumoniae". Trends Biochem. Sci. 25 (2): 50–2. doi:10.1016/S0968-0004(99)01530-3. PMID   10664582.
• Zhang QH, Ye M, Wu XY, et al. (2001). "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells". Genome Res. 10 (10): 1546–60. doi:10.1101/gr.140200. PMC   310934 . PMID   11042152.
• Borodovsky A, Ovaa H, Kolli N, et al. (2003). "Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family". Chem. Biol. 9 (10): 1149–59. doi: 10.1016/S1074-5521(02)00248-X . PMID   12401499.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Soares L, Seroogy C, Skrenta H, et al. (2004). "Two isoforms of otubain 1 regulate T cell anergy via GRAIL". Nat. Immunol. 5 (1): 45–54. doi:10.1038/ni1017. PMID   14661020. S2CID   27005972.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC   442148 . PMID   15231748.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Juris SJ, Shah K, Shokat K, et al. (2006). "Identification of otubain 1 as a novel substrate for the Yersinia protein kinase using chemical genetics and mass spectrometry". FEBS Lett. 580 (1): 179–83. Bibcode:2006FEBSL.580..179J. doi: 10.1016/j.febslet.2005.11.071 . PMID   16364312. S2CID   24766989.