POLDIP2

POLDIP2
Identifiers
Aliases	POLDIP2 , PDIP38, POLD4, p38, polymerase (DNA) delta interacting protein 2, DNA polymerase delta interacting protein 2
External IDs	OMIM: 611519; MGI: 1915061; HomoloGene: 9201; GeneCards: POLDIP2; OMA:POLDIP2 - orthologs
Gene location (Human) Chr. Chromosome 17 (human) [1] Band 17q11.2 Start 28,346,633 bp [1] End 28,357,527 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11|11 B5 Start 78,403,019 bp [2] End 78,413,562 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in gastrocnemius muscle muscle of thigh right adrenal gland right adrenal cortex left adrenal gland apex of heart left adrenal cortex right lobe of liver left ventricle right auricle of heart Top expressed in spermatocyte spermatid right kidney brown adipose tissue muscle of thigh vastus lateralis muscle proximal tubule tibialis anterior muscle ventricular zone triceps brachii muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding protein binding Cellular component mitochondrial nucleoid mitochondrion nucleus Biological process mitochondrion morphogenesis negative regulation of macroautophagy positive regulation of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 26073 67811 Ensembl ENSG00000004142 ENSMUSG00000001100 UniProt Q9Y2S7 Q91VA6 RefSeq (mRNA) NM_015584 NM_001290145 NM_026389 RefSeq (protein) NP_001277074 NP_056399 NP_080665 Location (UCSC) Chr 17: 28.35 – 28.36 Mb Chr 11: 78.4 – 78.41 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Polymerase delta-interacting protein 2 also known as Polymerase delta-interacting protein of 38 kDa (PDIP38) is encoded by the POLDIP2 gene in humans. ^{[5] [6]}

This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. The encoded protein also interacts with proliferating cell nuclear antigen. Some transcripts of this gene overlap in a tail-to-tail orientation with the gene for tumor necrosis factor, alpha-induced protein 1 (TNFAIP1). ^[6] POLDIP2 / PDIP38 is a moonlighting protein that has been identified in both the nucleus ^[7] and the mitochondrion ^{[8] [9]}

Interactions

In the nucleus, POLDIP2 has been shown to interact with PCNA, ^[5] while in the mitochondrion POLDIP2 has been shown to interact with proteins constituting the mitochondrial DNA nucleoid ^[10] and the AAA+ protease CLPXP ^{[9] [11]} where it modulates both the specificity and stability of this proteolytic machine.

References

1. GRCh38: Ensembl release 89: ENSG00000004142 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000001100 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Liu L, Rodriguez-Belmonte EM, Mazloum N, Xie B, Lee MY (March 2003). "Identification of a novel protein, PDIP38, that interacts with the p50 subunit of DNA polymerase delta and proliferating cell nuclear antigen". The Journal of Biological Chemistry. 278 (12): 10041–7. doi: 10.1074/jbc.M208694200 . PMID   12522211.
6. "Entrez Gene: POLDIP2 polymerase (DNA-directed), delta interacting protein 2".
7. Wong A, Zhang S, Mordue D, Wu JM, Zhang Z, Darzynkiewicz Z, et al. (October 2013). "PDIP38 is translocated to the spliceosomes/nuclear speckles in response to UV-induced DNA damage and is required for UV-induced alternative splicing of MDM2". Cell Cycle. 12 (19): 3184–93. doi:10.4161/cc.26221. PMC   3865014 . PMID   23989611.
8. Xie B, Li H, Wang Q, Xie S, Rahmeh A, Dai W, Lee MY (June 2005). "Further characterization of human DNA polymerase delta interacting protein 38". The Journal of Biological Chemistry. 280 (23): 22375–84. doi: 10.1074/jbc.M414597200 . PMID   15811854.
9. Strack PR, Brodie EJ, Zhan H, Schuenemann VJ, Valente LJ, Saiyed T, et al. (November 2020). "Polymerase delta-interacting protein 38 (PDIP38) modulates the stability and activity of the mitochondrial AAA+ protease CLPXP". Communications Biology. 3 (1): 646. doi:10.1038/s42003-020-01358-6. PMC   7647994 . PMID   33159171.
10. Cheng X, Kanki T, Fukuoh A, Ohgaki K, Takeya R, Aoki Y, et al. (December 2005). "PDIP38 associates with proteins constituting the mitochondrial DNA nucleoid". Journal of Biochemistry. 138 (6): 673–8. doi:10.1093/jb/mvi169. PMID   16428295.
11. Paredes F, Sheldon K, Lassègue B, Williams HC, Faidley EA, Benavides GA, et al. (February 2018). "Poldip2 is an oxygen-sensitive protein that controls PDH and αKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer". Proceedings of the National Academy of Sciences of the United States of America. 115 (8): 1789–1794. Bibcode:2018PNAS..115.1789P. doi: 10.1073/pnas.1720693115 . PMC   5828627 . PMID   29434038.

Further reading

• Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, et al. (October 2000). "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells". Genome Research. 10 (10): 1546–60. doi:10.1101/gr.140200. PMC   310934 . PMID   11042152.
• Hartley JL, Temple GF, Brasch MA (November 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC   310948 . PMID   11076863.
• Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (September 2000). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Reports. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC   1083732 . PMID   11256614.
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, et al. (October 2004). "From ORFeome to biology: a functional genomics pipeline". Genome Research. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC   528930 . PMID   15489336.
• Xie B, Li H, Wang Q, Xie S, Rahmeh A, Dai W, Lee MY (June 2005). "Further characterization of human DNA polymerase delta interacting protein 38". The Journal of Biological Chemistry. 280 (23): 22375–84. doi: 10.1074/jbc.M414597200 . PMID   15811854.
• Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, et al. (September 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl: 11858/00-001M-0000-0010-8592-0 . PMID   16169070. S2CID   8235923.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, et al. (January 2006). "The LIFEdb database in 2006". Nucleic Acids Research. 34 (Database issue): D415-8. doi:10.1093/nar/gkj139. PMC   1347501 . PMID   16381901.
• Klaile E, Müller MM, Kannicht C, Otto W, Singer BB, Reutter W, et al. (September 2007). "The cell adhesion receptor carcinoembryonic antigen-related cell adhesion molecule 1 regulates nucleocytoplasmic trafficking of DNA polymerase delta-interacting protein 38". The Journal of Biological Chemistry. 282 (36): 26629–40. doi: 10.1074/jbc.M701807200 . PMID   17623671.