Paleofeces (or palaeofaeces in British English) are ancient human feces, often found as part of archaeological excavations or surveys. The term coprolite is often used interchangeably, although coprolite can also refer to fossilized animal feces. Intact feces of ancient people may be found in caves in arid climates and in other locations with suitable preservation conditions. They are studied to determine the diet and health of the people who produced them through the analysis of seeds, small bones, and parasite eggs found inside. The feces can contain information about the person excreting the material as well as information about the material itself. They can also be chemically analyzed for more in-depth information on the individual who excreted them, using lipid analysis and ancient DNA analysis. The success rate of usable DNA extraction is relatively high in paleofeces, making it more reliable than skeletal DNA retrieval. [1]
The reason this analysis is possible at all is due to the digestive system not being entirely efficient, in the sense that not everything that passes through the digestive system is destroyed. Not all of the surviving material is recognizable, but some of it is. This material is generally the best indicator archaeologists can use to determine ancient diets, as no other part of the archaeological record is as direct an indicator. [2]
The process that preserves the feces in a way such that they can be analyzed later is called the Maillard reaction. This reaction creates a casing of sugar that preserves the feces from the elements. To extract and analyze the information contained within, researchers generally have to freeze the feces and grind it up into powder for analysis. [3]
Analysis of archaeological feces has a relatively short history compared to many other archaeological materials. The founder of the discipline is Dr. Eric O. Callen, who pioneered the subject in the late 1950s to mid-1960s. [4] His early papers used coprolite analysis to investigate early Mexican diets, published in The Prehistory of the Tehuacan Valley: Environment, and Subsistence. Despite his work showing promise, archaeological coprolite studies remained a niche topic, with few other researchers becoming involved. After Callen's sudden death in 1970, his work was continued by Vaughn Bryant at Texas A&M University, Department of Anthropology. Coprolite analysis gradually became a topic of serious study. Today coprolite analysis in archaeology has increased, and they have provided important evidence concerning the evolution of human health and diet, in the Americas and other parts of the world. [5] One of the most famous examples is the coprolite from Paisley Caves, Oregon, which has provided some of the earliest evidence for the human occupation of North America. [6]
A wide variety of methods can be used to analyse ancient feces, ranging from microscopic to molecular. At a basic level the analysis of size and morphology can provide some information on whether they are likely to be human or from another animal. Analyzed contents can include those visible to the naked eye, such as seeds and other plant remains—to the microscopic, including pollen and phytoliths. Parasites in coprolites can give information on the living conditions and health of ancient populations. [7] At the molecular level, ancient DNA analysis can be used both to identify the species and to provide dietary information. A method using lipid analysis can also be used for species identification, based on the range of fecal sterols and bile acids. [8] These molecules vary between species according to gut biochemistry, and so can distinguish between humans and other animals.
An example of researchers using paleofeces for the gathering of information using DNA analysis occurred at Hinds Cave in Texas by Hendrik Poinar and his team. The fecal samples obtained were over 2,000 years old. From the samples, Poinar was able to gather DNA samples using the analysis methods recounted above. From his research Poinar found that the feces belonged to three Native Americans, based on mtDNA similarities to present day Native Americans. Poinar also found DNA evidence of the food they ate. There were samples of buckthorn, acorns, ocotillo, nightshade and wild tobacco. No visible remnants of these plants were visible in the fecal matter. Along with plant material, there were also DNA sequences of animal species such as bighorn sheep, pronghorn antelope, and cottontail rabbit.
This analysis of the diet was very helpful. Previously it was assumed that this population of Native Americans survived with berries being their main source of nutrients. From the paleofeces, it was determined that these assumptions were incorrect and in the approximately 2 days of food that are represented in a fecal sample, 2–4 animal species and 4–8 plant species were represented. The nutritional diversity of this archaic human population was rather extraordinary. [1]
An example of the use of lipid analysis for identification of species is at the Neolithic site of Çatalhöyük in Turkey. Large midden deposits at the site are frequently found to contain fecal material [9] either as distinct coprolites or compressed 'cess pit' deposits. This was initially thought to be from dog on the basis of digested bone, however an analysis of the lipid profiles showed that many of the coprolites were actually from humans. [10]
The analysis of parasites from fecal material within cesspits has provided evidence for health and migration in past populations. For example, the identification of fish tapeworm eggs in Acre in the Crusader period indicate that this parasite was transported from northern Europe. The parasite was rarely seen in the Levant area during this time but was common in Northern Europe. It is suggested that it was brought to the region by the incoming Europeans. [11]
Zooarchaeology or archaeozoology merges the disciplines of zoology and archaeology, focusing on the analysis of animal remains within archaeological sites. This field, managed by specialists known as zooarchaeologists or faunal analysts, examines remnants such as bones, shells, hair, chitin, scales, hides, and proteins, such as DNA, to derive insights into historical human-animal interactions and environmental conditions. While bones and shells tend to be relatively more preserved in archaeological contexts, the survival of faunal remains is generally infrequent. The degradation or fragmentation of faunal remains presents challenges in the accurate analysis and interpretation of data.
Ascaris lumbricoides is a large parasitic roundworm of the genus Ascaris. It is the most common parasitic worm in humans. An estimated 807 million–1.2 billion people are infected with A. lumbricoides worldwide. People living in tropical and subtropical countries are at greater risk of infection. Infection by Ascaris lumbricoides infection is known as Ascariasis.
Archaeogenetics is the study of ancient DNA using various molecular genetic methods and DNA resources. This form of genetic analysis can be applied to human, animal, and plant specimens. Ancient DNA can be extracted from various fossilized specimens including bones, eggshells, and artificially preserved tissues in human and animal specimens. In plants, ancient DNA can be extracted from seeds and tissue. Archaeogenetics provides us with genetic evidence of ancient population group migrations, domestication events, and plant and animal evolution. The ancient DNA cross referenced with the DNA of relative modern genetic populations allows researchers to run comparison studies that provide a more complete analysis when ancient DNA is compromised.
A coprolite is fossilized feces. Coprolites are classified as trace fossils as opposed to body fossils, as they give evidence for the animal's behaviour rather than morphology. The name is derived from the Greek words κόπρος and λίθος. They were first described by William Buckland in 1829. Before this, they were known as "fossil fir cones" and "bezoar stones". They serve a valuable purpose in paleontology because they provide direct evidence of the predation and diet of extinct organisms. Coprolites may range in size from a few millimetres to over 60 centimetres.
Hendrik Nicholas Poinar is an evolutionary biologist specializing in ancient DNA. Poinar first became known for extracting DNA sequences from ground sloth coprolites. He is currently director of the Ancient DNA Centre at McMaster University in Hamilton, Ontario.
Paleogenetics is the study of the past through the examination of preserved genetic material from the remains of ancient organisms. Emile Zuckerkandl and Linus Pauling introduced the term in 1963, long before the sequencing of DNA, in reference to the possible reconstruction of the corresponding polypeptide sequences of past organisms. The first sequence of ancient DNA, isolated from a museum specimen of the extinct quagga, was published in 1984 by a team led by Allan Wilson.
Parasitic worms, also known as helminths, are large macroparasites; adults can generally be seen with the naked eye. Many are intestinal worms that are soil-transmitted and infect the gastrointestinal tract. Other parasitic worms such as schistosomes reside in blood vessels.
Ancient DNA (aDNA) is DNA isolated from ancient sources. Due to degradation processes ancient DNA is more degraded in comparison with contemporary genetic material. Genetic material has been recovered from paleo/archaeological and historical skeletal material, mummified tissues, archival collections of non-frozen medical specimens, preserved plant remains, ice and from permafrost cores, marine and lake sediments and excavation dirt.
5β-Coprostanol (5β-cholestan-3β-ol) is a 27-carbon stanol formed from the net reductive metabolism of cholesterol (cholest-5en-3β-ol) in the gut of most higher animals and birds. This compound has frequently been used as a biomarker for the presence of human faecal matter in the environment. 5β-coprostanol is thought to be exclusively bacterial in origin.
Human feces or faeces British English), commonly and in medical literature more often called stool, are the solid or semisolid remains of food that could not be digested or absorbed in the small intestine of humans, but has been further broken down by bacteria in the large intestine. It also contains bacteria and a relatively small amount of metabolic waste products such as bacterially altered bilirubin, and the dead epithelial cells from the lining of the gut. It is discharged through the anus during a process called defecation.
The Paisley Caves or the Paisley Five Mile Point Caves complex is a system of eight caves in an arid, desolate region of south-central Oregon, United States north of the present-day city of Paisley, Oregon. The caves are located in the Summer Lake basin at 4,520 feet (1,380 m) elevation and face west, carved into a ridge of Miocene and Pliocene era basalts mixed with soft volcanic tuffs and breccias by Pleistocene-era waves from Summer Lake. One of the caves may contain archaeological evidence of the oldest definitively-dated human presence in North America. The site was first studied by Luther Cressman in the 1930s.
Archaeoparasitology, a multi-disciplinary field within paleopathology, is the study of parasites in archaeological contexts. It includes studies of the protozoan and metazoan parasites of humans in the past, as well as parasites which may have affected past human societies, such as those infesting domesticated animals.
Paleoparasitology is the study of parasites from the past, and their interactions with hosts and vectors; it is a subfield of paleontology, the study of living organisms from the past. Some authors define this term more narrowly, as "Paleoparasitology is the study of parasites in archaeological material." K.J. Reinhard suggests that the term "archaeoparasitology" be applied to "... all parasitological remains excavated from archaeological contexts ... derived from human activity" and that "the term 'paleoparasitology' be applied to studies of nonhuman, paleontological material." This article follows Reinhard's suggestion and discusses the protozoan and animal parasites of non-human animals and plants from the past, while those from humans and our hominid ancestors are covered in archaeoparasitology.
Cestoda is a class of parasitic worms in the flatworm phylum (Platyhelminthes). Most of the species—and the best-known—are those in the subclass Eucestoda; they are ribbon-like worms as adults, known as tapeworms. Their bodies consist of many similar units known as proglottids—essentially packages of eggs which are regularly shed into the environment to infect other organisms. Species of the other subclass, Cestodaria, are mainly fish infecting parasites.
Feces are the solid or semi-solid remains of food that was not digested in the small intestine, and has been broken down by bacteria in the large intestine. Feces contain a relatively small amount of metabolic waste products such as bacterially altered bilirubin, and dead epithelial cells from the lining of the gut.
Wezmeh Cave is an archaeological site near Islamabad Gharb, western Iran, around 470 km (290 mi) southwest of the capital Tehran. The site was discovered in 1999 and excavated in 2001 by a team of Iranian archaeologists under the leadership of Dr. Kamyar Abdi. Wezmeh cave was re-excavated by a team under direction of Fereidoun Biglari in 2019.
Whale feces, the excrement of whales, has a vital role in the ecology of oceans, earning whales the title of "marine ecosystem engineers." This significant ecological role stems from the nutrients and compounds found in whale feces, which have far-reaching effects on marine life.
The diet of known human ancestors varies dramatically over time. Strictly speaking, according to evolutionary anthropologists and archaeologists, there is not a single hominin Paleolithic diet. The Paleolithic covers roughly 2.8 million years, concurrent with the Pleistocene, and includes multiple human ancestors with their own evolutionary and technological adaptations living in a wide variety of environments. This fact with the difficulty of finding conclusive evidence often makes broad generalizations of the earlier human diets very difficult. Humans' pre-hominin primate ancestors were broadly herbivorous, relying on either foliage or fruits and nuts and the shift in dietary breadth during the Paleolithic is often considered a critical point in hominin evolution. A generalization between Paleolithic diets of the various human ancestors that many anthropologists do make is that they are all to one degree or another omnivorous and are inextricably linked with tool use and new technologies.
Lisa-Marie Shillito is a British archaeologist and senior lecturer in landscape archaeology as well as director of the Wolfson Archaeology Laboratory and Earthslides at Newcastle University. Her practical work focuses on using soil micromorphology, phytolith analysis and geochemistry in order to understand human behaviour and landscape change. Her work includes the Neolithic settlements of Çatalhöyük in Turkey and Ness of Brodgar and Durrington Walls in Britain, but also Crusader castles and medieval settlements in Poland and the Baltic and in the Near East.
The evolution of the human oral microbiome is the study of microorganisms in the oral cavity and how they have adapted over time. There are recent advancements in ancient dental research that have given insight to the evolution of the human oral microbiome. Using these techniques it is now known what metabolite classes have been preserved and the difference in genetic diversity that exists from ancient to modern microbiota. The relationship between oral microbiota and its human host has changed and this transition can directly be linked to common diseases in human evolutionary past. Evolutionary medicine provides a framework for reevaluating oral health and disease and biological anthropology provides the context to identify the ancestral human microbiome. These disciplines together give insights into the oral microbiome and can potentially help contribute to restoring and maintaining oral health in the future.