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Plating efficiency ("PE") is a measure of the number of colonies originating from single cells. It is a very sensitive test and is often used for determining the nutritional requirements of cells, testing serum lots, measuring the effects of growth factors, and for toxicity testing.
Plating Efficiency is the number of cells that grow into colonies per 100 cells inoculated. That is, it is the proportion of cells that attach and grow to the number of cells originally plated, expressed as a percentage. PE can be determined by the following formulae:
or
The first method is more accurate.
Cell growth in culture generally undergoes a decline after plating, and graphically, PE is the global minimum (lowest point) of the growth curve at day one, after which growth rises again. The decrease in viable cells after plating is due to "anchorage-dependence"--cells must attach to the bottom of the culture dish.
Plating Efficiency is one of the parameters typically used to define growth properties of cells in culture. Other common parameters are doubling time ("DT") (which is an average of generation time ("GT")), and saturation density ("SD").
In physics, the cross section is a measure of the probability that a specific process will take place when some kind of radiant excitation intersects a localized phenomenon. For example, the Rutherford cross-section is a measure of probability that an alpha-particle will be deflected by a given angle during a collision with an atomic nucleus. Cross section is typically denoted σ (sigma) and is expressed in units of area, more specific in barns. In a way, it can be thought of as the size of the object that the excitation must hit in order for the process to occur, but more exactly, it is a parameter of a stochastic process.
Frequency is the number of occurrences of a repeating event per unit of time. It is also occasionally referred to as temporal frequency to emphasize the contrast to spatial frequency, and ordinary frequency to emphasize the contrast to angular frequency. Frequency is measured in hertz (Hz) which is equal to one event per second. The period is the duration of time of one cycle in a repeating event, so the period is the reciprocal of the frequency. For example, if a heart beats at a frequency of 120 times a minute, its period, T—the time interval between beats—is half a second. Frequency is an important parameter used in science and engineering to specify the rate of oscillatory and vibratory phenomena, such as mechanical vibrations, audio signals (sound), radio waves, and light.
Signal-to-noise ratio is a measure used in science and engineering that compares the level of a desired signal to the level of background noise. SNR is defined as the ratio of signal power to the noise power, often expressed in decibels. A ratio higher than 1:1 indicates more signal than noise.
The Pareto distribution, named after the Italian civil engineer, economist, and sociologist Vilfredo Pareto,, is a power-law probability distribution that is used in description of social, quality control, scientific, geophysical, actuarial, and many other types of observable phenomena. Originally applied to describing the distribution of wealth in a society, fitting the trend that a large portion of wealth is held by a small fraction of the population. The Pareto principle or "80-20 rule" stating that 80% of outcomes are due to 20% of causes was named in honour of Pareto, but the concepts are distinct, and only Pareto distributions with shape value of log45 ≈ 1.16 precisely reflect it. Empirical observation has shown that this 80-20 distribution fits a wide range of cases, including natural phenomena and human activities.
Exponential growth is a process that increases quantity over time. It occurs when the instantaneous rate of change of a quantity with respect to time is proportional to the quantity itself. Described as a function, a quantity undergoing exponential growth is an exponential function of time, that is, the variable representing time is the exponent.
An agar plate is a Petri dish that contains a growth medium solidified with agar, used to culture microorganisms. Sometimes selective compounds are added to influence growth, such as antibiotics.
Replica plating is a microbiological technique in which one or more secondary Petri plates containing different solid (agar-based) selective growth media are inoculated with the same colonies of microorganisms from a primary plate, reproducing the original spatial pattern of colonies. The technique involves pressing a velveteen-covered disk, and then imprinting secondary plates with cells in colonies removed from the original plate by the material. Generally, large numbers of colonies are replica plated due to the difficulty in streaking each out individually onto a separate plate.
A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as a research tool in molecular biology.
The mean corpuscular volume, or mean cell volume (MCV), is a measure of the average volume of a red blood corpuscle. The measure is obtained by multiplying a volume of blood by the proportion of blood that is cellular, and dividing that product by the number of erythrocytes in that volume. The mean corpuscular volume is a part of a standard complete blood count.
The term quantum efficiency (QE) may apply to incident photon to converted electron (IPCE) ratio of a photosensitive device, or it may refer to the TMR effect of a Magnetic Tunnel Junction.
Thermal shock is a type of rapidly transient mechanical load. By definition, it is a mechanical load caused by a rapid change of temperature of a certain point. It can be also extended to the case of a thermal gradient, which makes different parts of an object expand by different amounts. This differential expansion can be more directly understood in terms of strain, than in terms of stress, as it is shown in the following. At some point, this stress can exceed the tensile strength of the material, causing a crack to form. If nothing stops this crack from propagating through the material, it will cause the object's structure to fail.
The Luria–Delbrück experiment (1943) demonstrated that in bacteria, genetic mutations arise in the absence of selective pressure rather than being a response to it. Thus, it concluded Darwin's theory of natural selection acting on random mutations applies to bacteria as well as to more complex organisms. Max Delbrück and Salvador Luria won the 1969 Nobel Prize in Physiology or Medicine in part for this work.
The goodness of fit of a statistical model describes how well it fits a set of observations. Measures of goodness of fit typically summarize the discrepancy between observed values and the values expected under the model in question. Such measures can be used in statistical hypothesis testing, e.g. to test for normality of residuals, to test whether two samples are drawn from identical distributions, or whether outcome frequencies follow a specified distribution. In the analysis of variance, one of the components into which the variance is partitioned may be a lack-of-fit sum of squares.
In thermodynamics, the thermal efficiency is a dimensionless performance measure of a device that uses thermal energy, such as an internal combustion engine, a steam turbine or a steam engine, a boiler, furnace, or a refrigerator for example. For a heat engine, thermal efficiency is the fraction of the energy added by heat that is converted to net work output. In the case of a refrigeration or heat pump cycle, thermal efficiency is the ratio of net heat output for heating, or removal for cooling, to energy input.
A colony-forming unit is a unit used in microbiology. It estimates the number of bacteria or fungal cells in a sample which are viable, able to multiply via binary fission under the controlled conditions. Counting with colony-forming units requires culturing the microbes and counts only viable cells, in contrast with microscopic examination which counts all cells, living or dead. The visual appearance of a colony in a cell culture requires significant growth, and when counting colonies it is uncertain if the colony arose from one cell or a group of cells. Expressing results as colony-forming units reflects this uncertainty.
In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.
In pattern recognition, information retrieval and classification, precision and recall are performance metrics that apply to data retrieved from a collection, corpus or sample space.
In probability theory and statistics, the Poisson distribution, named after French mathematician Siméon Denis Poisson, is a discrete probability distribution that expresses the probability of a given number of events occurring in a fixed interval of time or space if these events occur with a known constant mean rate and independently of the time since the last event. The Poisson distribution can also be used for the number of events in other specified intervals such as distance, area or volume.
In the comparison of various statistical procedures, efficiency is a measure of quality of an estimator, of an experimental design, or of a hypothesis testing procedure. Essentially, a more efficient estimator, experiment, or test needs fewer observations than a less efficient one to achieve a given performance. This article primarily deals with efficiency of estimators.
Cell counting is any of various methods for the counting or similar quantification of cells in the life sciences, including medical diagnosis and treatment. It is an important subset of cytometry, with applications in research and clinical practice. For example, the complete blood count can help a physician to determine why a patient feels unwell and what to do to help. Cell counts within liquid media are usually expressed as a number of cells per unit of volume, thus expressing a concentration.