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Names | |
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Other names Seminaphtharhodafluorescein; Seminaphthorhodafluor | |
Identifiers | |
3D model (JSmol) | |
ChemSpider | |
PubChem CID | |
UNII | |
CompTox Dashboard (EPA) | |
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Properties | |
C24H15NO4 | |
Molar mass | 381.387 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). |
Seminaphtharhodafluor or SNARF is a fluorescent dye that changes color with pH. It can be used to construct optical biosensors that use enzymes that change pH.
The absorption peak of the derivative carboxy-SNARF[ specify ] at pH 6.0 is at wavelength (515 and) 550 nm, while that at pH 9.0 is at 575 nm.[ citation needed ]
The emission peak of carboxy-SNARF at pH 6.0 is at wavelength 585 nm, while that at pH 9.0 is at 640 nm. [1]
SNARF-1 can serve as a substrate for the MRP1 (multidrug resistance-associated protein-1) drug transporter, to measure the activity of the MRP1 transporter. For this purpose, an acetomethoxyester group is added to SNARF-1. Cellular esterases cleave off SNARF-1, and its transport out of the cells can be measured by following the loss of fluorescence from the cells. [2]