Related Research Articles
Size-exclusion chromatography, also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. The chromatography column is packed with fine, porous beads which are commonly composed of dextran, agarose, or polyacrylamide polymers. The pore sizes of these beads are used to estimate the dimensions of macromolecules. SEC is a widely used polymer characterization method because of its ability to provide good molar mass distribution (Mw) results for polymers.
A macromolecule is a very large molecule important to biological processes, such as a protein or nucleic acid. It is composed of thousands of covalently bonded atoms. Many macromolecules are polymers of smaller molecules called monomers. The most common macromolecules in biochemistry are biopolymers and large non-polymeric molecules such as lipids, nanogels and macrocycles. Synthetic fibers and experimental materials such as carbon nanotubes are also examples of macromolecules.
An ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds, capable of generating acceleration as high as 1 000 000 g. There are two kinds of ultracentrifuges, the preparative and the analytical ultracentrifuge. Both classes of instruments find important uses in molecular biology, biochemistry, and polymer science.
Theodor Svedberg was a Swedish chemist and Nobel laureate for his research on colloids and proteins using the ultracentrifuge. Svedberg was active at Uppsala University from the mid-1900s to late 1940s. While at Uppsala, Svedberg started as a docent before becoming the university's physical chemistry head in 1912. After leaving Uppsala in 1949, Svedberg was in charge of the Gustaf Werner Institute until 1967. Apart from his 1926 Nobel Prize, Svedberg was named a Foreign Member of the Royal Society in 1944 and became part of the National Academy of Sciences in 1945.
Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. The denser components of the mixture migrate away from the axis of the centrifuge, while the less dense components of the mixture migrate towards the axis. Chemists and biologists may increase the effective gravitational force of the test tube so that the precipitate (pellet) will travel quickly and fully to the bottom of the tube. The remaining liquid that lies above the precipitate is called a supernatant or supernate.
In biochemistry and cell biology, differential centrifugation is a common procedure used to separate organelles and other sub-cellular particles based on their sedimentation rate. Although often applied in biological analysis, differential centrifugation is a general technique also suitable for crude purification of non-living suspended particles. In a typical case where differential centrifugation is used to analyze cell-biological phenomena, a tissue sample is first lysed to break the cell membranes and release the organelles and cytosol. The lysate is then subjected to repeated centrifugations, where particles that sediment sufficiently quickly at a given centrifugal force for a given time form a compact "pellet" at the bottom of the centrifugation tube.
A shield-maiden was a female warrior from Scandinavian folklore and mythology.
Meols is a suburb in the Metropolitan Borough of Wirral, Merseyside, England. On the north coast of the Wirral Peninsula, it forms a contiguous built up area with the nearby town of Hoylake which lies to the west. Historically in Cheshire, since 1 April 1974 it has been part of the Metropolitan Borough of Wirral.
Thingwall is a village on the Wirral Peninsula, in Merseyside, England. The village is situated approximately 8 km (5.0 mi) to the south west of Birkenhead and 3 km (1.9 mi) north east of Heswall. Historically part of Cheshire, the area is within the Pensby and Thingwall Ward of the Metropolitan Borough of Wirral and the parliamentary constituency of Wirral West.
Håkon Grjotgardsson was the first Earl of Lade and an ally of Harald Fairhair, King of Norway.
In chemistry, the sedimentation coefficient of a particle characterizes its sedimentation during centrifugation. It is defined as the ratio of a particle's sedimentation velocity to the applied acceleration causing the sedimentation.
Avaldsnes is a village in Karmøy municipality in Rogaland county, Norway. The village is located on the northeastern part of the island of Karmøy, along the Karmsundet strait, just south of the town of Haugesund. The village was an ancient centre of power on the west coast of Norway and is the site of one of Norway's more important areas of cultural history. The trading port of Notow and the Avaldsnes Church are two notable historic sites in Avaldsnes.
The Pretenders is a dramatic play by Norwegian playwright Henrik Ibsen.
The Scottish–Norwegian War lasted from 1262 to 1266. The conflict arose because of disagreement over the ownership of the Hebrides. The war consisted of mainly skirmishes and feuds between the kings, and the only major battle was the indecisive Battle of Largs.
The term macromolecular assembly (MA) refers to massive chemical structures such as viruses and non-biologic nanoparticles, cellular organelles and membranes and ribosomes, etc. that are complex mixtures of polypeptide, polynucleotide, polysaccharide or other polymeric macromolecules. They are generally of more than one of these types, and the mixtures are defined spatially, and with regard to their underlying chemical composition and structure. Macromolecules are found in living and nonliving things, and are composed of many hundreds or thousands of atoms held together by covalent bonds; they are often characterized by repeating units. Assemblies of these can likewise be biologic or non-biologic, though the MA term is more commonly applied in biology, and the term supramolecular assembly is more often applied in non-biologic contexts. MAs of macromolecules are held in their defined forms by non-covalent intermolecular interactions, and can be in either non-repeating structures, or in repeating linear, circular, spiral, or other patterns. The process by which MAs are formed has been termed molecular self-assembly, a term especially applied in non-biologic contexts. A wide variety of physical/biophysical, chemical/biochemical, and computational methods exist for the study of MA; given the scale of MAs, efforts to elaborate their composition and structure and discern mechanisms underlying their functions are at the forefront of modern structure science.
Ingimundr, also known as Hingamund, Igmunt, Ingimund, was a tenth century Viking warlord. In 902, Irish sources record that the Vikings were driven from Dublin. It is almost certainly in the context of this exodus that Ingimundr appears on record. He is recorded to have led the abortive settlement of Norsemen on Anglesey, before being driven out from there as well. He appears to have then led his folk to the Wirral peninsula, where the English allowed him to settle his followers. Ingimundr's invasion of Anglesey may be the most notable Viking attack in Welsh history.
The Oseberg tapestry is a fragmentary tapestry, discovered within the Viking Oseberg ship burial in Norway.
Lagmann mac Gofraid may have been an early eleventh-century ruler of the Kingdom of the Isles. He seems to have been a son of Gofraid mac Arailt, King of the Isles, and was likely a member of the Uí Ímair kindred. According to mediaeval sources, Lagmann was closely associated with Óláfr Haraldsson, a future King of Norway. According one source, both men lent assistance to Knútr, son of Sveinn Haraldsson, King of Denmark, although it is possible that this account actually refers to Óláfr's campaigning in England several years beforehand. Lagmann and Óláfr are also recorded to have assisted Richard II, Duke of Normandy. The two are specified to have not only ravaged lands in Brittany on behalf of Richard, but were tasked to counter Richard's opponent Odo II, Count of Chartres. Lagmann's activities on the Continent may have arisen as a result of being forced from the Isles following the death of his possible brother Ragnall mac Gofraid, King of the Isles in 1004 or 1005. Lagmann's son, Amlaíb, is recorded to have perished at the Battle of Clontarf in 1014. If Lagmann died at about this time as well, it could account for the record of Hákon Eiríksson assuming control of the Isles.
Turi Emma King is a Canadian-British professor of Public Engagement and Genetics at the University of Leicester. In 2012, King led the DNA verification during the exhumation and reburial of Richard III of England. She is also known for featuring with Stacey Dooley on the BBC Two genealogy series, DNA Family Secrets. She is currently the Director of the Milner Centre for Evolution at the University of Bath.
Anne Hiltner was an American polymer scientist who founded the Center for Applied Polymer Research (CAPRI) and was later instrumental in the founding of the Center for Layer Polymeric Systems (CLiPS), a National Science Foundation Science and Technology Center at Case Western Reserve University. She served as Director of the Center for Layered Polymeric Systems from its founding in 2006 until her death in 2010.
References
- ↑ Stephen Harding - The University of Nottingham. Retrieved 17 January 2012.
- 1 2 "People - Stephen E. Harding". School of Biosciences. The University of Nottingham. Retrieved 20 August 2024.
- ↑ Harding, Stephen Ernest (1980). Modelling biological macromolecules in solution: The general tri-axial ellipsoid (PhD thesis). University of Leicester. Retrieved 20 August 2024.
- ↑ "Prof Stephen Harding". Wirral Archaeology. 5 January 2023. Retrieved 20 August 2024.
- ↑ "Saving Oseberg - Kulturhistorisk museum". www.khm.uio.no (in Norwegian). Retrieved 2017-08-02.
- ↑ "Tildelinger av ordener og medaljer". www.kongehuset.no (in Norwegian). Retrieved 2017-08-02.
- ↑ University of Nottingham (2012-04-05), University scientist and Viking expert made a Knight of Norway , retrieved 2017-08-02
- ↑ Largs & District Historical Society (2016-09-01). "Science and the Vikings". YouTube. Retrieved 2017-10-12.
- ↑ "Svedberg Lecture" (PDF). doi:10.1007/s00249-018-1321-3.
