Sulforhodamine 101

Last updated
Sulforhodamine 101
Sulforhodamine 101 structure.png
Identifiers
3D model (JSmol)
3582548
ECHA InfoCard 100.056.491 OOjs UI icon edit-ltr-progressive.svg
PubChem CID
UNII
  • [O-]S(C(C=C(S(O)(=O)=O)C=C1)=C1C2=C(C=C3C4=C5CCCN4CCC3)C5=[O+]C6=C(CCCN7CCC8)C7=C8C=C62)(=O)=O
Properties
C31H30N2O7S2
Molar mass 606.71 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Yes check.svgY  verify  (what is  Yes check.svgYX mark.svgN ?)

Sulforhodamine 101 (SR101) is a red fluorescent dye. In neurophysiological experiments which comprise calcium imaging methods, it can be used for a counterstaining of astrocytes to be able to analyze data from neurons separately. [1] However, in addition to labeling astrocytes, SR101 labels myelinating oligodendrocytes. [2] SR101 has been reported to affect excitability of neurons and should therefore be used with caution. [3]

A sulfonyl chloride derivative of sulforhodamine 101, known as Texas Red, is used for conjugation to a number of functional groups, especially primary amines.

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References

  1. Nimmerjahn, A.; Kirchhoff, F.; Kerr, J.N.; Helmchen, F. (2004). "Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo". Nature Methods . 1 (1): 31–7. doi:10.1038/nmeth706. PMID   15782150. S2CID   2073853. Closed Access logo transparent.svg
  2. Hill, R.A.; Grutzendler, J. (2014). "In vivo imaging of oligodendrocytes with sulforhodamine 101". Nature Methods . 11 (11): 1081–1082. doi:10.1038/nmeth.3140. PMC   4539948 . PMID   25357236. Closed Access logo transparent.svg
  3. Kang, J.; Kang N.; Yu, Y.; Zhang, J.; et al. (2010). "Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons". Neuroscience . 169 (4): 1601–1609. doi:10.1016/j.neuroscience.2010.06.020. PMC   2918738 . PMID   20600669.