Ulrike Endesfelder

Last updated

Ulrike Endesfelder
Born (1983-07-04) 4 July 1983 (age 40)
NationalityGerman
Alma mater
Known for Single-Molecule Microbiology
Scientific career
Fields Biophysics, Cell Biology, Single Molecule Techniques, Super-Resolution Microscopy [1]
Institutions
Thesis Quantitative localization-based super-resolution microscopy: Concepts and applications [2]  (2012)
Website Endesfelder Lab

Ulrike Endesfelder (born 4 July 1983) is a German physicist known for her work in Single-Molecule Microbiology and Super-resolution microscopy. She is the Group Leader of the Research Group Endesfelder [3] and full professor (W3) at the Institute for microbiology and biotechnology at the University of Bonn in Bonn, Germany. [3]

Contents

Early life and education

Endesfelder was born on 4 July 1983. [4] From 2002 to 2008, she studied Physics, Astronomy, and Microbiology at the University of Bonn in Bonn, Germany and Stony Brook University, NY, USA. In 2012, she earned her Ph.D. in Physics at the Department of Applied Laser Physics and Laser Spectroscopy at Bielefeld University, Germany. [2] [5]

Career and research

For her postgraduate work, Endesfelder worked as a postdoctoral researcher at the Department of Biotechnology and Biophysics, University of Würzburg (2012/13) and at the Department of Physical and Theoretical Chemistry, Goethe University Frankfurt (2013/14).

Between 2014 and 2020, Endesfelder was a Group Leader at the Department of Systems and Synthetic Microbiology at the Max Planck Institute for Terrestrial Microbiology in Marburg, Germany.

Subsequently, Endesfelder relocated to the United States to work as an Associate Professor (tenure track) at the Department of Physics at the Carnegie Mellon University in Pittsburgh, PA between 2020 and 2021. [6]

Since 2021, Endesfelder has been a Full Professor (W3) at the Institute for Microbiology & Biotechnology at the University of Bonn, Germany.

Endesfelder's group uses tailored quantitative single-molecule tracking and structural super-resolution microscopy methods to understand molecular processes in cells. [7] They are particularly keen on exploring microbial cell biology using fluorescence microscopy and biophysical methods. [8]

Endesfelder is a well-known microscopist and biophysicist. [9] [10] [11] [12] [13] Hence, she is frequently invited to speak at workshops [14] [15] and conferences [16] [17] in the field.

Since 2021, Endesfelder has been an associate editor of Biophysical Reports, [18] an open-access journal run by the Biophysical Society.

Awards and honours

Related Research Articles

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References

  1. "Ulrike Endesfelder: Die Junge Akademie". Startseite (in German). Retrieved 4 August 2023.
  2. 1 2 "Prof. Dr. Ulrike Endesfelder". AcademiaNet. 9 December 2015. Retrieved 4 August 2023.
  3. 1 2 "Overview". IfMB. 6 July 2022. Retrieved 4 August 2023.
  4. "Ulrike Endesfelder – Laser Teaching Center".
  5. "Dr. Ulrike Endesfelder". DPG (in German). 17 December 2019. Retrieved 4 August 2023.
  6. "Ulrike EndesfelderDepartment of Physics". Carnegie Mellon. Retrieved 4 August 2023.
  7. "Ulrike Endesfelder". Universität Bonn (in German). 6 July 2022. Retrieved 4 August 2023.
  8. "Research Focus". IfMB. 6 July 2022. Retrieved 4 August 2023.
  9. Endesfelder, Ulrike; Malkusch, Sebastian; Fricke, Franziska; Heilemann, Mike (13 February 2014). "A simple method to estimate the average localization precision of a single-molecule localization microscopy experiment". Histochemistry and Cell Biology. 141 (6): 629–638. doi:10.1007/s00418-014-1192-3. ISSN   0948-6143. PMID   24522395. S2CID   253886169.
  10. Endesfelder, Ulrike; Finan, Kieran; Holden, Seamus J.; Cook, Peter R.; Kapanidis, Achillefs N.; Heilemann, Mike (2013). "Multiscale Spatial Organization of RNA Polymerase in Escherichia coli". Biophysical Journal. 105 (1): 172–181. Bibcode:2013BpJ...105..172E. doi:10.1016/j.bpj.2013.05.048. ISSN   0006-3495. PMC   3699759 . PMID   23823236.
  11. Endesfelder, Ulrike; Malkusch, Sebastian; Flottmann, Benjamin; Mondry, Justine; Liguzinski, Piotr; Verveer, Peter J.; Heilemann, Mike (April 2011). "Chemically Induced Photoswitching of Fluorescent Probes—A General Concept for Super-Resolution Microscopy". Molecules. 16 (4): 3106–3118. doi: 10.3390/molecules16043106 . ISSN   1420-3049. PMC   6260607 . PMID   21490558.
  12. Endesfelder, Ulrike; Heilemann, Mike (30 October 2014). "Direct Stochastic Optical Reconstruction Microscopy (DSTORM)". Advanced Fluorescence Microscopy. Methods in Molecular Biology. Vol. 1251. New York, NY: Springer New York. pp. 263–276. doi:10.1007/978-1-4939-2080-8_14. ISBN   978-1-4939-2079-2. ISSN   1064-3745. PMID   25391804.
  13. Endesfelder, Ulrike; Heilemann, Mike (27 February 2014). "Art and artifacts in single-molecule localization microscopy: beyond attractive images". Nature Methods. 11 (3): 235–238. doi:10.1038/nmeth.2852. ISSN   1548-7091. PMID   24577272. S2CID   52858968.
  14. "Advanced fluorescence imaging techniques". EMBL.org. 7 May 2023. Retrieved 4 August 2023.
  15. "The Biophysical Society > Meetings & Events > Special Sessions > Workshops". biophysics.org. 11 September 2019. Retrieved 4 August 2023.
  16. "TiM2023". German BioImaging. 9 March 2023. Retrieved 4 August 2023.
  17. "Visualizing cellular life: From single cell imaging to in vivo single-molecule biochemistry". Abbelight. 22 February 1999. Retrieved 4 August 2023.
  18. Zimmerman, Meredith (28 April 2023). "Know the Editor: Ulrike Endesfelder". The Biophysical Society. Retrieved 4 August 2023.
  19. "Dr. Ulrike Endesfelder elected as member of the Junge Akademie". mpi-marburg.mpg.de (in German). 19 July 2023. Retrieved 4 August 2023.