The 1940 City of Chester by-election was a by-election to the United Kingdom House of Commons for the City of Chester constituency, which occurred following the death of Sir Charles Cayzer. The by-election was unopposed being held by the Conservatives.
| Party | Candidate | Votes | % | ±% | |
|---|---|---|---|---|---|
| Conservative | Basil Nield | Unopposed | N/A | N/A | |
| Conservative hold | |||||
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