The defending champions were Virginia Ruano Pascual and Paola Suárez, but Suárez had to withdraw due to a back injury.
Lisa Raymond and Samantha Stosur were the defending champions, but lost in the semifinals to Katarina Srebotnik and Ai Sugiyama.
Yan Zi and Zheng Jie were the defending champions but Zheng did not compete. Yan partnered with Peng Shuai but lost in the quarterfinals to Alicia Molik and Mara Santangelo.
Virginia Ruano Pascual and Paola Suárez were the defending champions, but Suárez did not participate due to injury. Ruano Pascual partnered Conchita Martínez, but lost in the first round to Jennifer Hopkins and Mashona Washington.
Katarina Srebotnik and Bob Bryan were the defending champions, but Srebtonik did not compete in the Mixed Doubles tournament at this U.S. Open. Bryan partnered Vera Zvonareva, and successfully defended the title, defeating Alicia Molik and Todd Woodbridge in the final 6–3, 6–4.
Kim Clijsters and Ai Sugiyama were the defending champions, however Clijsters did not compete.
Leander Paes and Martina Navratilova were the defending champions but lost in the third round to Wayne and Cara Black.
Alicia Molik and Mara Santangelo were the defending champions, but lost in the first round to Sara Errani and Bethanie Mattek.
Lindsay Davenport and Lisa Raymond were the defending champions but only Raymond competed that year with Martina Navratilova.
Vera Zvonareva and Bob Bryan were the defending champions, but Zvonareva did not participate in this U.S. Open due to injury. Bryan partnered Rennae Stubbs, and the pair lost in the quarterfinals to Corina Morariu and Mike Bryan.
Corina Morariu and Larisa Neiland were the defending champions but did not return to defend their title.
Defending champions Lisa Raymond and Mike Bryan lost in the first round to Arantxa Sánchez Vicario and Daniel Nestor.
Cara Black and Rennae Stubbs were the defending champions and successfully defended their title, defeating Daniela Hantuchová and Ai Sugiyama 6–7(6–8), 7–6(7–4), 6–3 in the final.
Rennae Stubbs and Todd Woodbridge were the defending champions, but lost in the first round to Martina Navratilova and Leander Paes.
Nadia Petrova and Meghann Shaughnessy were the defending champions, but lost in quarterfinals to Lisa Raymond and Rennae Stubbs.
Lindsay Davenport and Lisa Raymond were the defending champions, but Davenport did not compete this year in order to focus on the singles tournament.
Kim Clijsters and Ai Sugiyama were the defending champions, but Clijsters withdrew from the tournament due to a left ankle injury. Sugiyama teamed up with Liezel Huber and lost in the quarterfinals to Dinara Safina and Meghann Shaughnessy.
Lisa Raymond and Rennae Stubbs were the defending champions, but competed this year with different partners.
Cara Black and Rennae Stubbs were the defending champions, but lost in the first round to Elena Dementieva and Flavia Pennetta.
Alicia Molik and Lisa Raymond were the defending champions, but Molik chose not to compete in 2005; Raymond played alongside Samantha Stosur, but lost in the final.
Cara Black 
Rennae Stubbs 
Anna-Lena Grönefeld 
