Colin Fleming and Ross Hutchins were the defending champions, but decided not to participate.
Michail Elgin and Nikolaus Moser were the defending champions but Moser decided not to participate.
Elgin partnered up with Alexandre Kudryavtsev, but they were eliminated by Radu Albot and Artem Smirnov in the quarterfinals.
Konstantin Kravchuk and Denys Molchanov were the defending champions, but Molchanov chose not to compete Kravchuk played with Teymuraz Gabashvili but lost in the quarterfinals.
Riccardo Ghedin and Claudio Grassi won in the final against Andrey Golubev and Mikhail Kukushkin 3–6, 6–3, [10–8].
Sergey Betov and Alexander Bury were the defending champions, but Bury decided not to participate. Betov teamed up with Michail Elgin and successfully defended the title, beating Denys Molchanov and Franko Škugor in the final, 6–3, 7–5.
Jared Donaldson and Stefan Kozlov are the defending champions but only Kozlov defending his title partnering Taylor Fritz, losing in the first round to Toshihide Matsui and Dean O'Brien.
Yuki Bhambri and Adrián Menéndez-Maceiras were the defending champions but chose not to participate.
Renzo Olivo and Horacio Zeballos were the defending champions, but chose not to defend their title.
The 2016 Fergana Challenger was a professional tennis tournament played on hard courts. It was the 17th edition of the tournament for men which was part of the 2016 ATP Challenger Tour, offering a total of $50,000 in prize money, and the sixth edition of the event for women on the 2016 ITF Women's Circuit, offering a total of $25,000 in prize money. It took place in Fergana, Uzbekistan, on 13–19 June 2016.
Konstantin Kravchuk and Denys Molchanov were the defending champions but lost in the first round to Toshihide Matsui and Vishnu Vardhan.
This was the first edition of the tournament.
Enrique López-Pérez and Jeevan Nedunchezhiyan were the defending champions but chose not to defend their title.
Ariel Behar and Andrey Golubev were the defending champions but only Behar chose to defend his title, partnering Aliaksandr Bury. Behar lost in the quarterfinals to Victor Vlad Cornea and Zdeněk Kolář.
Yannick Jankovits and Luca Margaroli were the defending champions but chose not to defend their title.
Yaraslav Shyla and Andrei Vasilevski were the defending champions but only Shyla chose to defend his title, partnering Vladyslav Manafov. Shyla lost in the quarterfinals to Evgeny Karlovskiy and Evgeny Tyurnev.
Marco Chiudinelli and Marius Copil were the defending champions but chose not to defend their title.
Denys Molchanov and Sergiy Stakhovsky were the defending champions but chose not to defend their title.
Laurynas Grigelis and Zdeněk Kolář were the defending champions but chose not to defend their title.
Hans Podlipnik-Castillo and Andrei Vasilevski were the defending champions but chose not to defend their title.
Toshihide Matsui and Vishnu Vardhan were the defending champions but only Matsui chose to defend his title, partnering Saketh Myneni. Matsui lost in the first round to Ivan Gakhov and Roman Safiullin.
Hans Podlipnik Castillo and Andrei Vasilevski were the defending champions but only Vasilevski chose to defend his title, partnering Vladyslav Manafov. Vasilevski lost in the first round to Federico Gaio and Enrique López Pérez.
Yannick Jankovits 
Luca Margaroli 
Toshihide Matsui 
Vishnu Vardhan 
