 | This article relies largely or entirely on a single source .(June 2025) |
| 2016 Milo Open Cali | |
|---|---|
| Date | 4–10 July |
| Edition | 9th |
| Surface | Clay |
| Location | Cali, Colombia |
| Champions | |
| Singles | |
 Darian King | |
| Doubles | |
 Nicolás Jarry / Hans Podlipnik | |
The 2016 Milo Open Cali was a professional tennis tournament played on clay courts. It was the ninth edition of the tournament, which is part of the 2016 ATP Challenger Tour. It took place in Cali, Colombia between 4 and 10 July 2016.
| Country | Player | Rank | Seed |
|---|---|---|---|
 DOM | Víctor Estrella Burgos | 77 | 1 |
 COL | Alejandro González | 154 | 2 |
| CHI | Gonzalo Lama | 161 | 3 |
| COL | Eduardo Struvay | 162 | 4 |
 BRA | João Souza | 183 | 5 |
 ESA | Marcelo Arévalo | 218 | 6 |
| BAR | Darian King | 255 | 7 |
 ARG | Juan Ignacio Londero | 258 | 8 |
The following players received wildcards into
Giovanni Lapentti Felipe Mantilla Nicolás Mejía Cristian Rodríguez The following player entered the singles main draw with a protected ranking:
The following players received entry from the qualifying draw:
| Country | Player | Country | Player | Rank1 | Seed |
|---|---|---|---|---|---|
| ESA | Marcelo Arévalo | BRA | João Souza | 289 | 1 |
| COL | Nicolás Barrientos |  VEN | Roberto Maytín | 345 | 2 |
| CHI | Nicolás Jarry | CHI | Hans Podlipnik | 542 | 3 |
 GUA | Christopher Díaz Figueroa | VEN | Luis David Martínez | 581 | 4 |
The following pairs received wildcards into the singles main draw:
José Daniel Bendeck / Nicolás Mejía Alejandro Gómez / Felipe Mantilla Juan Sebastián Gómez / Cristian Rodríguez Darian King def. Víctor Estrella Burgos, 5–7, 6–4, 7–5 [1] Nicolás Jarry / Hans Podlipnik def. 
Erik Crepaldi / Daniel Dutra da Silva, 6–1, 7–6(8–6)