| 2016 Samarkand Challenger | |
|---|---|
| Date | 9–14 May |
| Edition | 20th |
| Draw | 32S / 16D |
| Prize money | $50,000 |
| Surface | Clay |
| Location | Samarkand, Uzbekistan |
| Champions | |
| Singles | |
 Karen Khachanov | |
| Doubles | |
Denis Matsukevich /  Andrei Vasilevski | |
The 2016 Samarkand Challenger was a professional tennis tournament played on clay courts. It was the 20th edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Samarkand, Uzbekistan between 9 and 14 May 2016.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
| RUS | Karen Khachanov | 125 | 1 |
 MDA | Radu Albot | 143 | 2 |
 ISR | Amir Weintraub | 192 | 3 |
 ESP | Jordi Samper-Montaña | 216 | 4 |
 UZB | Farrukh Dustov | 220 | 5 |
 KAZ | Dmitry Popko | 225 | 6 |
 SRB | Nikola Milojević | 233 | 7 |
| ESP | Enrique López Pérez | 235 | 8 |
The following players received wildcards into the singles main draw:
Sanjar Fayziev Temur Ismailov Shonigmatjon Shofayziyev Pavel TsoyThe following players received entry from the qualifying draw:
Bar Tzuf Botzer Mikhail Elgin Markos Kalovelonis 
Francesco Vilardo Karen Khachanov def. Rubén Ramírez Hidalgo, 6–1, 6–7(6–8), 6–1 Denis Matsukevich / Andrei Vasilevski def. 
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