| 2016 Challenger Ciudad de Guayaquil | |
|---|---|
| Date | 31 October – 6 November |
| Edition | 12th |
| Surface | Clay |
| Location | Guayaquil, Ecuador |
| Champions | |
| Singles | |
 Nicolás Kicker | |
| Doubles | |
 Ariel Behar /  Fabiano de Paula | |
The 2016 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the twelfth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Guayaquil, Ecuador, between October 31 and November 6, 2016.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 POR | Gastão Elias | 57 | 1 |
| ARG | Facundo Bagnis | 67 | 2 |
| ARG | |||
| ARG | Carlos Berlocq | 89 | 4 |
 DOM | Víctor Estrella Burgos | 94 | 5 |
 COL | Santiago Giraldo | 100 | 6 |
| BRA | Rogério Dutra Silva | 105 | 7 |
| BRA | João Souza | 125 | 8 |
 USA | Ernesto Escobedo | 126 | 9 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Daniel Elahi Galán 
Bastián Malla Andrés Molteni João Pedro Sorgi The following player entered as a lucky loser:
Nicolás Kicker def. 
Arthur De Greef 6–3, 6–2 Ariel Behar / Fabiano de Paula def. 
Marcelo Arévalo / 
Sergio Galdós 6–2, 6–4The 2009 Challenger Ciudad de Guayaquil was a professional tennis tournament played on outdoor red clay courts. It was the fifth edition of the tournament which was part of the 2009 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 9 and 15 November 2009.
The 2010 Challenger Ciudad de Guayaquil was a professional tennis tournament played on outdoor red clay courts. It was the sixth edition of the tournament which is part of the 2010 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 8 and 14 November 2010.
The 2011 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the seventh edition of the tournament which is part of the 2011 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 21 and 27 November 2011.
The 2012 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the eighth edition of the tournament, which was part of the 2012 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 5 and 10 November 2012.
The 2013 Challenger Ciudad de Guayaquil was a professional clay-court tennis tournament. It was the ninth edition of the tournament, which was part of the 2013 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 11 and 17 November 2013.
The 2014 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the tenth edition of the tournament which is part of the 2014 ATP Challenger Tour. It took place in Guayaquil, Ecuador between November 10 and November 16, 2014.
The 2015 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the eleventh edition of the tournament which was part of the 2015 ATP Challenger Tour. It took place in Guayaquil, Ecuador between November 2 and November 8, 2015.
The 2016 Santiago Challenger was a professional tennis tournament played on clay courts. It was the second edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Santiago, Chile between 17 and 22 October 2016.
The 2016 Canberra Tennis International is a professional tennis tournament played on outdoor hard courts. It is the second edition of the tournament which was part of the 2016 ATP Challenger Tour and the 2016 ITF Women's Circuit. It took place in Canberra, Australia between 31 October and 6 November 2016.
The 2016 WHB Hungarian Open was a professional tennis tournament played on indoor hardcourt. It was the inaugural edition of the tournament and was part of the 2016 ATP Challenger Tour. It took place in Budapest, Hungary between 24 and 30 October 2016.
The 2016 Bauer Watertechnology Cup was a professional tennis tournament played on carpet courts. It was the 20th edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Eckental, Germany between 31 October and 6 November 2016.
The 2016 Knoxville Challenger was a professional tennis tournament played on indoor hard courts. It was the thirteenth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Knoxville, United States between 6 and 13 November 2016.
The 2017 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the thirteenth edition of the tournament which was part of the 2017 ATP Challenger Tour. It took place in Guayaquil, Ecuador between October 30 and November 4, 2017.
The 2018 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the fourteenth edition of the tournament which was part of the 2018 ATP Challenger Tour. It took place in Guayaquil, Ecuador between October 29 and November 3, 2018.
The 2019 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the fifteenth edition of the tournament which was part of the 2019 ATP Challenger Tour. It took place in Guayaquil, Ecuador between October 28 and November 3, 2019.
The 2020 Challenger Ciudad de Guayaquil is a professional tennis tournament played on clay courts. It is the sixteenth edition of the tournament which is part of the 2020 ATP Challenger Tour. It will take place in Guayaquil, Ecuador between 16 and 23 November 2020.
The 2021 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the seventeenth edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 1 and 7 November 2021.
The 2022 Salinas Challenger, also known as Challenger de Salinas Copa Banco Guayaquil presentado por CNT for sponsorship reasons, was a professional tennis tournament played on hard courts. It was the 22nd edition of the tournament which was part of the 2022 ATP Challenger Tour. It took place in Salinas, Ecuador between 4 and 10 April 2022.
The 2022 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the 18th edition of the tournament which was part of the 2022 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 31 October and 6 November 2022.
The 2023 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the 19th edition of the tournament which was part of the 2023 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 30 October and 5 November 2023.
