| 2016 Karshi Challenger | |
|---|---|
| Date | 2–7 May |
| Edition | 10th |
| Draw | 32S / 16D |
| Prize money | $50,000+H |
| Surface | Hard |
| Location | Qarshi, Uzbekistan |
| Champions | |
| Singles | |
 Marko Tepavac | |
| Doubles | |
 Enrique López Pérez /  Jeevan Nedunchezhiyan | |
The 2016 Karshi Challenger was a professional tennis tournament played on hard courts. It was the tenth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Qarshi, Uzbekistan between 2 and 7 May 2016.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 ISR | Dudi Sela | 78 | 1 |
 RUS | Karen Khachanov | 128 | 2 |
 MDA | Radu Albot | 151 | 3 |
 KAZ | Aleksandr Nedovyesov | 197 | 4 |
| ISR | Amir Weintraub | 198 | 5 |
 UKR | Denys Molchanov | 224 | 6 |
| KAZ | Dmitry Popko | 230 | 7 |
| RUS | Aslan Karatsev | 216 | 8 |
The following players received wildcards into the singles main draw:
Pavel Tsoy Sanjar Fayziev Temur Ismailov Shonigmatjon ShofayziyevThe following players received entry from the qualifying draw:
Sergey Betov Denis Matsukevich 
Francesco Vilardo Richard Muzaev The following player received entry as a lucky loser:
Marko Tepavac def. Dudi Sela, 2–6, 6–3, 7–6(7–4) Enrique López Pérez / Jeevan Nedunchezhiyan def. 
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