| 2020 Prosperita Open | |
|---|---|
| Date | 31 August – 6 September |
| Edition | 17th |
| Draw | 32S / 16D |
| Surface | Clay |
| Location | Ostrava, Czech Republic |
| Champions | |
| Singles | |
 Aslan Karatsev | |
| Doubles | |
 Artem Sitak /  Igor Zelenay | |
The 2020 Prosperita Open was a professional tennis tournament played on clay courts. It was the 17th edition of the tournament which was part of the 2020 ATP Challenger Tour. It took place in Ostrava, Czech Republic between 31 August and 6 September.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 SUI | Henri Laaksonen | 129 | 1 |
 BLR | Ilya Ivashka | 138 | 2 |
 KOR | Chung Hyeon | 142 | 3 |
 LAT | Ernests Gulbis | 158 | 4 |
| SVK | Martin Kližan | 160 | 5 |
 FRA | |||
 CAN | Steven Diez | 165 | 7 |
 UKR | Sergiy Stakhovsky | 168 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as special exempts:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
Aslan Karatsev def. 
Oscar Otte 6–4, 6–2. Artem Sitak / Igor Zelenay def. 
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