AP2M1

AP2M1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1H6E, 2JKT, 2JKR, 2VGL, 2BP5
Identifiers
Aliases	AP2M1 , AP50, CLAPM1, mu2, adaptor related protein complex 2 mu 1 subunit, adaptor related protein complex 2 subunit mu 1, MRD60
External IDs	OMIM: 601024; MGI: 1298405; HomoloGene: 3000; GeneCards: AP2M1; OMA:AP2M1 - orthologs
Gene location (Human) Chr. Chromosome 3 (human) [1] Band 3q27.1 Start 184,174,689 bp [1] End 184,184,214 bp [1]
Gene location (Mouse) Chr. Chromosome 16 (mouse) [2] Band 16|16 A3 Start 20,354,228 bp [2] End 20,363,659 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right frontal lobe prefrontal cortex right hemisphere of cerebellum right adrenal gland right adrenal cortex stromal cell of endometrium left adrenal gland left adrenal cortex cingulate gyrus ganglionic eminence Top expressed in primary visual cortex Cortex of frontal lobe olfactory bulb superior frontal gyrus dentate gyrus of hippocampal formation granule cell neural layer of retina neural tube mesencephalon hippocampus proper hypothalamus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function lipid binding clathrin adaptor activity transporter activity transmembrane transporter binding protein binding signal sequence binding low-density lipoprotein particle receptor binding Cellular component extracellular exosome lysosomal membrane AP-2 adaptor complex endocytic vesicle membrane cytosol membrane endolysosome membrane clathrin adaptor complex clathrin-coated pit clathrin-coated endocytic vesicle membrane plasma membrane clathrin-coated endocytic vesicle Biological process ephrin receptor signaling pathway mitigation of host defenses by virus clathrin-dependent endocytosis negative regulation of protein localization to plasma membrane antigen processing and presentation of exogenous peptide antigen via MHC class II endocytosis protein transport vesicle-mediated transport intracellular protein transport Wnt signaling pathway, planar cell polarity pathway microtubule-based movement membrane organization low-density lipoprotein particle receptor catabolic process low-density lipoprotein particle clearance transport vesicle budding from membrane receptor internalization regulation of vesicle size Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1173 11773 Ensembl ENSG00000161203 ENSMUSG00000022841 UniProt Q96CW1 P84091 RefSeq (mRNA) NM_004068 NM_001025205 NM_001311198 NM_009679 NM_001302970 RefSeq (protein) NP_001020376 NP_001298127 NP_004059 NP_001289899 NP_033809 Location (UCSC) Chr 3: 184.17 – 184.18 Mb Chr 16: 20.35 – 20.36 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

AP-2 complex subunit mu is a protein that in humans is encoded by the AP2M1 gene. ^[5]

Function

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. ^[6]

Interactions

AP2M1 has been shown to interact with CTLA-4 ^{[7] [8]} and Alpha-1B adrenergic receptor. ^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000161203 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000022841 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Druck T, Gu Y, Prabhala G, Cannizzaro LA, Park SH, Huebner K, Keen JH (Nov 1995). "Chromosome localization of human genes for clathrin adaptor polypeptides AP2 beta and AP50 and the clathrin-binding protein, VCP". Genomics. 30 (1): 94–7. doi:10.1006/geno.1995.0016. PMID   8595912.
6. "Entrez Gene: AP2M1 adaptor-related protein complex 2, mu 1 subunit".
7. Follows ER, McPheat JC, Minshull C, Moore NC, Pauptit RA, Rowsell S, Stacey CL, Stanway JJ, Taylor IW, Abbott WM (Oct 2001). "Study of the interaction of the medium chain mu 2 subunit of the clathrin-associated adapter protein complex 2 with cytotoxic T-lymphocyte antigen 4 and CD28". The Biochemical Journal. 359 (Pt 2): 427–34. doi:10.1042/0264-6021:3590427. PMC   1222163 . PMID   11583591.
8. Chuang E, Alegre ML, Duckett CS, Noel PJ, Vander Heiden MG, Thompson CB (Jul 1997). "Interaction of CTLA-4 with the clathrin-associated protein AP50 results in ligand-independent endocytosis that limits cell surface expression". Journal of Immunology. 159 (1): 144–51. doi: 10.4049/jimmunol.159.1.144 . PMID   9200449.
9. Diviani D, Lattion AL, Abuin L, Staub O, Cotecchia S (May 2003). "The adaptor complex 2 directly interacts with the alpha 1b-adrenergic receptor and plays a role in receptor endocytosis". The Journal of Biological Chemistry. 278 (21): 19331–40. doi: 10.1074/jbc.M302110200 . PMID   12644451.

Further reading

• Ohno H, Stewart J, Fournier MC, Bosshart H, Rhee I, Miyatake S, Saito T, Gallusser A, Kirchhausen T, Bonifacino JS (Sep 1995). "Interaction of tyrosine-based sorting signals with clathrin-associated proteins". Science. 269 (5232): 1872–5. Bibcode:1995Sci...269.1872O. doi:10.1126/science.7569928. PMID   7569928.
• Liu Q, Feng Y, Forgac M (Dec 1994). "Activity and in vitro reassembly of the coated vesicle (H+)-ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2". The Journal of Biological Chemistry. 269 (50): 31592–7. doi: 10.1016/S0021-9258(18)31735-6 . PMID   7989329.
• Pauloin A, Thurieau C (Dec 1993). "The 50 kDa protein subunit of assembly polypeptide (AP) AP-2 adaptor from clathrin-coated vesicles is phosphorylated on threonine-156 by AP-1 and a soluble AP50 kinase which co-purifies with the assembly polypeptides". The Biochemical Journal. 296 (2): 409–15. doi:10.1042/bj2960409. PMC   1137711 . PMID   8257432.
• Chang MP, Mallet WG, Mostov KE, Brodsky FM (May 1993). "Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits". The EMBO Journal. 12 (5): 2169–80. doi:10.1002/j.1460-2075.1993.tb05865.x. PMC   413438 . PMID   8491205.
• Wang X, Kilimann MW (Jan 1997). "Identification of two new mu-adaptin-related proteins, mu-ARP1 and mu-ARP2". FEBS Letters. 402 (1): 57–61. doi: 10.1016/S0014-5793(96)01500-1 . PMID   9013859. S2CID   81844290.
• Chuang E, Alegre ML, Duckett CS, Noel PJ, Vander Heiden MG, Thompson CB (Jul 1997). "Interaction of CTLA-4 with the clathrin-associated protein AP50 results in ligand-independent endocytosis that limits cell surface expression". Journal of Immunology. 159 (1): 144–51. doi: 10.4049/jimmunol.159.1.144 . PMID   9200449.
• Zhang Y, Allison JP (Aug 1997). "Interaction of CTLA-4 with AP50, a clathrin-coated pit adaptor protein". Proceedings of the National Academy of Sciences of the United States of America. 94 (17): 9273–8. Bibcode:1997PNAS...94.9273Z. doi: 10.1073/pnas.94.17.9273 . PMC   23153 . PMID   9256472.
• Ohno H, Aguilar RC, Fournier MC, Hennecke S, Cosson P, Bonifacino JS (Nov 1997). "Interaction of endocytic signals from the HIV-1 envelope glycoprotein complex with members of the adaptor medium chain family". Virology. 238 (2): 305–15. doi: 10.1006/viro.1997.8839 . PMID   9400603.
• Santini F, Marks MS, Keen JH (May 1998). "Endocytic clathrin-coated pit formation is independent of receptor internalization signal levels". Molecular Biology of the Cell. 9 (5): 1177–94. doi:10.1091/mbc.9.5.1177. PMC   25339 . PMID   9571248.
• Owen DJ, Evans PR (Nov 1998). "A structural explanation for the recognition of tyrosine-based endocytotic signals". Science. 282 (5392): 1327–32. Bibcode:1998Sci...282.1327O. doi:10.1126/science.282.5392.1327. PMC   5600252 . PMID   9812899.
• Berlioz-Torrent C, Shacklett BL, Erdtmann L, Delamarre L, Bouchaert I, Sonigo P, Dokhelar MC, Benarous R (Feb 1999). "Interactions of the cytoplasmic domains of human and simian retroviral transmembrane proteins with components of the clathrin adaptor complexes modulate intracellular and cell surface expression of envelope glycoproteins". Journal of Virology. 73 (2): 1350–61. doi:10.1128/JVI.73.2.1350-1361.1999. PMC   103959 . PMID   9882340.
• Teuchert M, Berghöfer S, Klenk HD, Garten W (Dec 1999). "Recycling of furin from the plasma membrane. Functional importance of the cytoplasmic tail sorting signals and interaction with the AP-2 adaptor medium chain subunit". The Journal of Biological Chemistry. 274 (51): 36781–9. doi: 10.1074/jbc.274.51.36781 . PMID   10593987.
• Umeda A, Meyerholz A, Ungewickell E (May 2000). "Identification of the universal cofactor (auxilin 2) in clathrin coat dissociation". European Journal of Cell Biology. 79 (5): 336–42. doi:10.1078/S0171-9335(04)70037-0. PMID   10887964.
• Jullien-Flores V, Mahé Y, Mirey G, Leprince C, Meunier-Bisceuil B, Sorkin A, Camonis JH (Aug 2000). "RLIP76, an effector of the GTPase Ral, interacts with the AP2 complex: involvement of the Ral pathway in receptor endocytosis" (PDF). Journal of Cell Science. 113 (16): 2837–44. doi:10.1242/jcs.113.16.2837. PMID   10910768.
• Wyss S, Berlioz-Torrent C, Boge M, Blot G, Höning S, Benarous R, Thali M (Mar 2001). "The highly conserved C-terminal dileucine motif in the cytosolic domain of the human immunodeficiency virus type 1 envelope glycoprotein is critical for its association with the AP-1 clathrin adaptor [correction of adapter]". Journal of Virology. 75 (6): 2982–92. doi:10.1128/JVI.75.6.2982-2992.2001. PMC   115924 . PMID   11222723.
• Morris SM, Cooper JA (Feb 2001). "Disabled-2 colocalizes with the LDLR in clathrin-coated pits and interacts with AP-2". Traffic. 2 (2): 111–23. doi: 10.1034/j.1600-0854.2001.020206.x . PMID   11247302. S2CID   33186168.
• Follows ER, McPheat JC, Minshull C, Moore NC, Pauptit RA, Rowsell S, Stacey CL, Stanway JJ, Taylor IW, Abbott WM (Oct 2001). "Study of the interaction of the medium chain mu 2 subunit of the clathrin-associated adapter protein complex 2 with cytotoxic T-lymphocyte antigen 4 and CD28". The Biochemical Journal. 359 (Pt 2): 427–34. doi:10.1042/0264-6021:3590427. PMC   1222163 . PMID   11583591.
• Friocourt G, Chafey P, Billuart P, Koulakoff A, Vinet MC, Schaar BT, McConnell SK, Francis F, Chelly J (Sep 2001). "Doublecortin interacts with mu subunits of clathrin adaptor complexes in the developing nervous system". Molecular and Cellular Neurosciences. 18 (3): 307–19. doi:10.1006/mcne.2001.1022. PMID   11591131. S2CID   54264008.

External links

• Human AP2M1 genome location and AP2M1 gene details page in the UCSC Genome Browser .