CPSF1

CPSF1
Identifiers
Aliases	CPSF1 , CPSF160, HSU37012, P/cl.18, cleavage and polyadenylation specific factor 1
External IDs	OMIM: 606027; MGI: 2679722; HomoloGene: 40865; GeneCards: CPSF1; OMA:CPSF1 - orthologs
Gene location (Human) Chr. Chromosome 8 (human) [1] Band 8q24.3 Start 144,393,229 bp [1] End 144,409,349 bp [1]
Gene location (Mouse) Chr. Chromosome 15 (mouse) [2] Band 15|15 D3 Start 76,480,003 bp [2] End 76,491,791 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right testis left testis right hemisphere of cerebellum right uterine tube granulocyte pituitary gland anterior pituitary duodenum mucosa of transverse colon spleen Top expressed in internal carotid artery external carotid artery hair follicle primitive streak vestibular membrane of cochlear duct endothelial cell of lymphatic vessel epiblast neural layer of retina ventricular zone adrenal gland More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleic acid binding enzyme binding protein binding mRNA 3'-UTR AU-rich region binding RNA binding Cellular component mRNA cleavage and polyadenylation specificity factor complex nucleus nucleoplasm Biological process mRNA splicing, via spliceosome termination of RNA polymerase II transcription mRNA polyadenylation mRNA processing mRNA cleavage mRNA 3'-end processing mRNA export from nucleus pre-mRNA cleavage required for polyadenylation tRNA splicing, via endonucleolytic cleavage and ligation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 29894 94230 Ensembl ENSG00000071894 ENSMUSG00000034022 UniProt Q10570 Q9EPU4 RefSeq (mRNA) NM_013291 NM_001164173 NM_053193 NM_001361485 RefSeq (protein) NP_037423 NP_001157645 NP_444423 NP_001348414 Location (UCSC) Chr 8: 144.39 – 144.41 Mb Chr 15: 76.48 – 76.49 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Cleavage and polyadenylation specificity factor subunit 1 is a protein that in humans is encoded by the CPSF1 gene. ^{[5] [6] [7]}

In most cases eukaryotic pre-messenger(m)RNA 3 prime ends are processed in two coordinated steps. First there is a site-specific cleavage by an endonuclease and then the addition of a poly(A) tail at the 3 prime end of the 5 prime cleavage product. Cleavage requires four multisubunit complexes, namely cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), cleavage factors Im and IIm (CFIm and CFIIm), along with a single subunit poly(A)polymerase (PAP). CPSF1 is the largest component of the CPSF complex composed of CPSF1, CPSF2, CPSF3, CPSF4, FIP1L1, Symplekin and WDR33 and located in the nucleus. ^[8]

References

1. GRCh38: Ensembl release 89: ENSG00000071894 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000034022 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Jenny A, Keller W (September 1995). "Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor". Nucleic Acids Res. 23 (14): 2629–35. doi:10.1093/nar/23.14.2629. PMC   307085 . PMID   7651824.
6. Murthy KG, Manley JL (December 1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes Dev. 9 (21): 2672–83. doi: 10.1101/gad.9.21.2672 . PMID   7590244.
7. "Entrez Gene: CPSF1 cleavage and polyadenylation specific factor 1, 160kDa".
8. Shi Y, Di Giammartino DC, Taylor D, Sarkeshik A, Rice WJ, Yates JR, Frank J, Manley JL (February 2009). "Molecular architecture of the human pre-mRNA 3' processing complex". Mol. Cell. 33 (3): 365–76. doi:10.1016/j.molcel.2008.12.028. PMC   2946185 . PMID   19217410.

Further reading

• Keller W, Bienroth S, Lang KM, Christofori G (1992). "Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3' processing signal AAUAAA". EMBO J. 10 (13): 4241–9. doi:10.1002/j.1460-2075.1991.tb05002.x. PMC   453176 . PMID   1756731.
• Thuresson AC, Aström J, Aström A, et al. (1994). "Multiple forms of poly(A) polymerases in human cells". Proc. Natl. Acad. Sci. U.S.A. 91 (3): 979–83. Bibcode:1994PNAS...91..979T. doi: 10.1073/pnas.91.3.979 . PMC   521437 . PMID   8302877.
• Schul W, Groenhout B, Koberna K, et al. (1996). "The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA". EMBO J. 15 (11): 2883–92. doi:10.1002/j.1460-2075.1996.tb00649.x. PMC   450226 . PMID   8654386.
• McCracken S, Fong N, Yankulov K, et al. (1997). "The C-terminal domain of RNA polymerase II couples mRNA processing to transcription". Nature. 385 (6614): 357–61. Bibcode:1997Natur.385..357M. doi:10.1038/385357a0. PMID   9002523. S2CID   4307073.
• Dantonel JC, Murthy KG, Manley JL, Tora L (1997). "Transcription factor TFIID recruits factor CPSF for formation of 3' end of mRNA". Nature. 389 (6649): 399–402. Bibcode:1997Natur.389..399D. doi:10.1038/38763. PMID   9311784. S2CID   4413324.
• Takagaki Y, Manley JL (2000). "Complex Protein Interactions within the Human Polyadenylation Machinery Identify a Novel Component". Mol. Cell. Biol. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. PMC   85326 . PMID   10669729.
• de Vries H, Rüegsegger U, Hübner W, et al. (2000). "Human pre-mRNA cleavage factor IIm contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC   305781 . PMID   11060040.
• Samiotaki M, Balatsos NA, Courtis N, Tsiapalis CM (2001). "Assignment of the 160-kDa subunit of cleavage and polyadenylation specificity factor (CPSF1) to human chromosome 8q24.23 by radiation hybrid mapping". Cytogenet. Cell Genet. 90 (3–4): 234–5. doi:10.1159/000056776. PMID   11124521. S2CID   202645825.
• McCracken S, Lambermon M, Blencowe BJ (2002). "SRm160 Splicing Coactivator Promotes Transcript 3′-End Cleavage". Mol. Cell. Biol. 22 (1): 148–60. doi:10.1128/MCB.22.1.148-160.2002. PMC   134228 . PMID   11739730.
• Maeda Y, Ito M, Harashima N, et al. (2002). "Cleavage and polyadenylation specificity factor (CPSF)-derived peptides can induce HLA-A2-restricted and tumor-specific CTLs in the majority of gastrointestinal cancer patients". Int. J. Cancer. 99 (3): 409–17. doi:10.1002/ijc.10377. PMID   11992410. S2CID   12789683.
• Holland L, Gauthier L, Bell-Rogers P, Yankulov K (2002). "Distinct parts of minichromosome maintenance protein 2 associate with histone H3/H4 and RNA polymerase II holoenzyme". Eur. J. Biochem. 269 (21): 5192–202. doi: 10.1046/j.1432-1033.2002.03224.x . PMID   12392551.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Lehner B, Semple JI, Brown SE, et al. (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. 83 (1): 153–67. doi:10.1016/S0888-7543(03)00235-0. PMID   14667819.
• Kaufmann I, Martin G, Friedlein A, et al. (2005). "Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase". EMBO J. 23 (3): 616–26. doi:10.1038/sj.emboj.7600070. PMC   1271804 . PMID   14749727.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi: 10.1073/pnas.0404720101 . PMC   514446 . PMID   15302935.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.

External links

• Human CPSF1 genome location and CPSF1 gene details page in the UCSC Genome Browser .