CPSF3

CPSF3
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	2I7T, 2I7V
Identifiers
Aliases	CPSF3 , CPSF-73, CPSF73, cleavage and polyadenylation specific factor 3
External IDs	OMIM: 606029 MGI: 1859328 HomoloGene: 6499 GeneCards: CPSF3
Gene location (Human)
Chr.	Chromosome 2 (human)
End	9,473,101 bp
Gene location (Mouse)
Chr.	Chromosome 12 (mouse)
End	21,365,057 bp
RNA expression pattern
	Top expressed in
	ganglionic eminence; ; endothelial cell; ; sperm; ; monocyte; ; stromal cell of endometrium; ; islet of Langerhans; ; pancreatic epithelial cell; ; Achilles tendon; ; spongy bone; ; internal globus pallidus;
	Top expressed in
	primitive streak; ; medullary collecting duct; ; ureter; ; internal carotid artery; ; condyle; ; fossa; ; vas deferens; ; external carotid artery; ; facial motor nucleus; ; renal corpuscle;
	More reference expression data
	n/a
Gene ontology
Molecular function	5'-3' exonuclease activity ; metal ion binding ; endoribonuclease activity ; protein binding ; RNA binding ; nuclease activity ; endonuclease activity ; hydrolase activity ;
Cellular component	mRNA cleavage and polyadenylation specificity factor complex ; nucleoplasm ; nucleus ;
Biological process	mRNA splicing, via spliceosome ; termination of RNA polymerase II transcription ; mRNA 3'-end processing by stem-loop binding and cleavage ; mRNA processing ; mRNA export from nucleus ; mRNA polyadenylation ; mRNA cleavage ; mRNA 3'-end processing ; RNA phosphodiester bond hydrolysis, endonucleolytic ; sno(s)RNA 3'-end processing ; snoRNA splicing ; pre-mRNA cleavage required for polyadenylation ;
	Sources:Amigo / QuickGO
Orthologs
	51692
	54451
	ENSG00000119203
	ENSMUSG00000054309
	Q9UKF6
	Q9QXK7
	NM_016207 ; NM_001321833 ; NM_001321834 ; NM_001321835 ; NM_001321836 Contents References ; External links ; Further reading ;
	NM_018813 ; NM_001364372 ; NM_001364373
	NP_001308762 ; NP_001308763 ; NP_001308764 ; NP_001308765 ; NP_057291
	NP_061283 ; NP_001351301 ; NP_001351302
	Wikidata
View/Edit Human	View/Edit Mouse

Last updated March 28, 2024

Cleavage and polyadenylation specificity factor subunit 3 is a protein that in humans is encoded by the CPSF3 gene.^[5]^[6]

Related Research Articles

Polyadenylation is the addition of a poly(A) tail to an RNA transcript, typically a messenger RNA (mRNA). The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. In many bacteria, the poly(A) tail promotes degradation of the mRNA. It, therefore, forms part of the larger process of gene expression.

Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3' signaling region from a newly synthesized pre-messenger RNA (pre-mRNA) molecule in the process of gene transcription. In eukaryotes, messenger RNA precursors (pre-mRNA) are transcribed in the nucleus from DNA by the enzyme, RNA polymerase II. The pre-mRNA must undergo post-transcriptional modifications, forming mature RNA (mRNA), before they can be transported into the cytoplasm for translation into proteins. The post-transcriptional modifications are: the addition of a 5' m7G cap, splicing of intronic sequences, and 3' cleavage and polyadenylation.

DNA-directed RNA polymerases I, II, and III subunit RPABC3 is a protein that in humans is encoded by the POLR2H gene.

Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene.

Cyclin-C is a protein that in humans is encoded by the CCNC gene.

Transcription initiation factor TFIID subunit 2 is a protein that in humans is encoded by the TAF2 gene.

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene.

Cleavage and polyadenylation specificity factor subunit 5 (CPSF5) is an enzyme that in humans is encoded by the NUDT21 gene. It belongs to the Nudix family of hydrolases.

Cleavage and polyadenylation specificity factor subunit 2 is a protein that in humans is encoded by the CPSF2 gene. This protein is a subunit of the cleavage and polyadenylation specificity factor (CPSF) complex which plays a key role in pre-mRNA 3' end processing and polyadenylation. The CPSF2 protein connects the two subunits of the complex, mCF and mPSF. Its structure contributes both to the stability of the subunits interaction and to the flexibility of the complex necessary for function. This protein has been identified as an essential subunit of the complex as certain mutations in the region inhibit CPSF complex formation.

Cleavage and polyadenylation specificity factor subunit 1 is a protein that in humans is encoded by the CPSF1 gene.

Transcription initiation factor TFIID subunit 13 is a protein that in humans is encoded by the TAF13 gene.

Poly(A) polymerase alpha is an enzyme that in humans is encoded by the PAPOLA gene.

Cleavage stimulation factor 50 kDa subunit is a protein that in humans is encoded by the CSTF1 gene.

Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene.

Cleavage and polyadenylation specificity factor subunit 4 is a protein that in humans is encoded by the CPSF4 gene.

Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene.

Integrator complex subunit 11 is a protein that in humans is encoded by the CPSF3L gene.

DNA-directed RNA polymerase III subunit RPC5 is an enzyme that in humans is encoded by the POLR3E gene.

Poly(A) polymerase gamma is an enzyme that in humans is encoded by the PAPOLG gene.

Cleavage and polyadenylation specificity factor subunit 7 is a protein that in humans is encoded by the CPSF7 gene.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000119203 - Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000054309 - Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Jenny A, Minvielle-Sebastia L, Preker PJ, Keller W (Dec 1996). "Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I". Science. 274 (5292): 1514–7. Bibcode:1996Sci...274.1514J. doi:10.1126/science.274.5292.1514. PMID 8929409. S2CID 12958377.
↑ "Entrez Gene: CPSF3 cleavage and polyadenylation specific factor 3, 73kDa".

External links

Human CPSF3 genome location and CPSF3 gene details page in the UCSC Genome Browser .

Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes Dev. 9 (21): 2672–83. doi: 10.1101/gad.9.21.2672 . PMID 7590244.
Jenny A, Hauri HP, Keller W (1994). "Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit". Mol. Cell. Biol. 14 (12): 8183–90. doi:10.1128/MCB.14.12.8183. PMC 359357 . PMID 7969155.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Thuresson AC, Aström J, Aström A, et al. (1994). "Multiple forms of poly(A) polymerases in human cells". Proc. Natl. Acad. Sci. U.S.A. 91 (3): 979–83. Bibcode:1994PNAS...91..979T. doi: 10.1073/pnas.91.3.979 . PMC 521437 . PMID 8302877.
McCracken S, Fong N, Yankulov K, et al. (1997). "The C-terminal domain of RNA polymerase II couples mRNA processing to transcription". Nature. 385 (6614): 357–61. Bibcode:1997Natur.385..357M. doi:10.1038/385357a0. PMID 9002523. S2CID 4307073.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Takagaki Y, Manley JL (2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component". Mol. Cell. Biol. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. PMC 85326 . PMID 10669729.
de Vries H, Rüegsegger U, Hübner W, et al. (2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC 305781 . PMID 11060040.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC 139241 . PMID 12477932.
Kaufmann I, Martin G, Friedlein A, et al. (2005). "Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase". EMBO J. 23 (3): 616–26. doi:10.1038/sj.emboj.7600070. PMC 1271804 . PMID 14749727.
Calzado MA, Sancho R, Muñoz E (2004). "Human immunodeficiency virus type 1 Tat increases the expression of cleavage and polyadenylation specificity factor 73-kilodalton subunit modulating cellular and viral expression". J. Virol. 78 (13): 6846–54. doi:10.1128/JVI.78.13.6846-6854.2004. PMC 421638 . PMID 15194760.
Jin J, Smith FD, Stark C, et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. Bibcode:2004CBio...14.1436J. doi: 10.1016/j.cub.2004.07.051 . PMID 15324660.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928 . PMID 15489334.
Mandel CR, Kaneko S, Zhang H, et al. (2007). "Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease". Nature. 444 (7121): 953–6. doi:10.1038/nature05363. PMC 3866582 . PMID 17128255.
de la Vega L, Sánchez-Duffhues G, Fresno M, et al. (2007). "The 73 kDa subunit of the CPSF complex binds to the HIV-1 LTR promoter and functions as a negative regulatory factor that is inhibited by the HIV-1 Tat protein". J. Mol. Biol. 372 (2): 317–30. doi:10.1016/j.jmb.2007.06.075. PMID 17669424.

This article on a gene on human chromosome 2 is a stub. You can help Wikipedia by expanding it.

This page is based on this Wikipedia article
Text is available under the CC BY-SA 4.0 license; additional terms may apply.
Images, videos and audio are available under their respective licenses.

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000119203 - Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000054309 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid8929409-5] Jenny A, Minvielle-Sebastia L, Preker PJ, Keller W (Dec 1996). "Sequence similarity between the 73-kilodalton protein of mammalian CPSF and a subunit of yeast polyadenylation factor I". Science. 274 (5292): 1514–7. Bibcode:1996Sci...274.1514J. doi:10.1126/science.274.5292.1514. PMID 8929409. S2CID 12958377.

[entrez-6] "Entrez Gene: CPSF3 cleavage and polyadenylation specific factor 3, 73kDa".

[1]

[2]

[3]

[4]

[5]

[6]

CPSF3

Contents

Related Research Articles

References

External links

Further reading