CSTF1

CSTF1
Identifiers
Aliases	CSTF1 , CstF-50, CstFp50, cleavage stimulation factor subunit 1
External IDs	OMIM: 600369; MGI: 1914587; HomoloGene: 1012; GeneCards: CSTF1; OMA:CSTF1 - orthologs
Gene location (Human)
Chr.	Chromosome 20 (human)
End	56,406,362 bp
Gene location (Mouse)
Chr.	Chromosome 2 (mouse)
End	172,224,368 bp
RNA expression pattern
	Top expressed in
	testicle; ; secondary oocyte; ; Achilles tendon; ; gonad; ; islet of Langerhans; ; stromal cell of endometrium; ; ventricular zone; ; rectum; ; granulocyte; ; body of pancreas;
	Top expressed in
	spermatid; ; seminiferous tubule; ; spermatocyte; ; yolk sac; ; epiblast; ; ventricular zone; ; secondary oocyte; ; Ileal epithelium; ; tail of embryo; ; embryo;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	protein binding ; RNA binding ;
Cellular component	nucleus ; nucleoplasm ; mRNA cleavage stimulating factor complex ;
Biological process	mRNA splicing, via spliceosome ; termination of RNA polymerase II transcription ; mRNA processing ; mRNA 3'-end processing ;
	Sources:Amigo / QuickGO
Orthologs
	1477
	67337
	ENSG00000101138
	ENSMUSG00000027498
	Q05048
	Q99LC2
	NM_001033521 ; NM_001033522 ; NM_001324
	NM_024199
	NP_001028693 ; NP_001028694 ; NP_001315
	NP_077161
	Wikidata
View/Edit Human	View/Edit Mouse

Last updated October 09, 2024

Cleavage stimulation factor 50 kDa subunit is a protein that in humans is encoded by the CSTF1 gene.^[5]^[6]^[7]

This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.^[7]

Interactions

CSTF1 has been shown to interact with BARD1.^[6]^[8]

Related Research Articles

Polyadenylation is the addition of a poly(A) tail to an RNA transcript, typically a messenger RNA (mRNA). The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation. In many bacteria, the poly(A) tail promotes degradation of the mRNA. It, therefore, forms part of the larger process of gene expression.

Transcriptional modification or co-transcriptional modification is a set of biological processes common to most eukaryotic cells by which an RNA primary transcript is chemically altered following transcription from a gene to produce a mature, functional RNA molecule that can then leave the nucleus and perform any of a variety of different functions in the cell. There are many types of post-transcriptional modifications achieved through a diverse class of molecular mechanisms.

Cleavage stimulatory factor or cleavage stimulation factor is a heterotrimeric protein, made up of the proteins CSTF1 (55kDa), CSTF2 (64kDa) and CSTF3 (77kDa), totalling about 200 kDa. It is involved in the cleavage of the 3' signaling region from a newly synthesized pre-messenger RNA (mRNA) molecule. CstF is recruited by cleavage and polyadenylation specificity factor (CPSF) and assembles into a protein complex on the 3' end to promote the synthesis of a functional polyadenine tail, which results in a mature mRNA molecule ready to be exported from the cell nucleus to the cytosol for translation.

Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3' signaling region from a newly synthesized pre-messenger RNA (pre-mRNA) molecule in the process of gene transcription. In eukaryotes, messenger RNA precursors (pre-mRNA) are transcribed in the nucleus from DNA by the enzyme, RNA polymerase II. The pre-mRNA must undergo post-transcriptional modifications, forming mature RNA (mRNA), before they can be transported into the cytoplasm for translation into proteins. The post-transcriptional modifications are: the addition of a 5' m7G cap, splicing of intronic sequences, and 3' cleavage and polyadenylation.

DNA-directed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that is encoded by the POLR2A gene in humans.

BRCA1-associated RING domain protein 1 is a protein that in humans is encoded by the BARD1 gene. The human BARD1 protein is 777 amino acids long and contains a RING finger domain, four ankyrin repeats, and two tandem BRCT domains.

Splicing factor U2AF 35 kDa subunit is a protein that in humans is encoded by the U2AF1 gene.

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene.

Ubiquitin-conjugating enzyme E2 D1 is a protein that in humans is encoded by the UBE2D1 gene.

Cleavage and polyadenylation specificity factor subunit 5 (CPSF5) is an enzyme that in humans is encoded by the NUDT21 gene. It belongs to the Nudix family of hydrolases.

Cleavage and polyadenylation specificity factor subunit 2 is a protein that in humans is encoded by the CPSF2 gene. This protein is a subunit of the cleavage and polyadenylation specificity factor (CPSF) complex which plays a key role in pre-mRNA 3' end processing and polyadenylation. The CPSF2 protein connects the two subunits of the complex, mCF and mPSF. Its structure contributes both to the stability of the subunits interaction and to the flexibility of the complex necessary for function. This protein has been identified as an essential subunit of the complex as certain mutations in the region inhibit CPSF complex formation.

Cleavage and polyadenylation specificity factor subunit 1 is a protein that in humans is encoded by the CPSF1 gene.

Poly(A) polymerase alpha is an enzyme that in humans is encoded by the PAPOLA gene.

Symplekin is a protein that in humans is encoded by the SYMPK gene.

Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene.

Cleavage stimulation factor 64 kDa subunit, tau variant is a protein that in humans is encoded by the CSTF2T gene.

Cleavage and polyadenylation specificity factor subunit 3 is a protein that in humans is encoded by the CPSF3 gene.

Cleavage and polyadenylation specificity factor subunit 4 is a protein that in humans is encoded by the CPSF4 gene.

Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene.

Cleavage and polyadenylation specificity factor subunit 7 is a protein that in humans is encoded by the CPSF7 gene.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000101138 – Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000027498 – Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Takagaki Y, Manley JL (November 1992). "A human polyadenylation factor is a G protein beta-subunit homologue". The Journal of Biological Chemistry. 267 (33): 23471–4. doi: 10.1016/S0021-9258(18)35861-7 . PMID 1358884.
1 2 Kleiman FE, Manley JL (March 2001). "The BARD1-CstF-50 interaction links mRNA 3' end formation to DNA damage and tumor suppression". Cell. 104 (5): 743–53. doi: 10.1016/S0092-8674(01)00270-7 . PMID 11257228. S2CID 15022237.
1 2 "Entrez Gene: CSTF1 cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa".
↑ Kleiman FE, Manley JL (September 1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50". Science. 285 (5433): 1576–9. doi:10.1126/science.285.5433.1576. PMID 10477523.

External links

Human CSTF1 genome location and CSTF1 gene details page in the UCSC Genome Browser .

Takagaki Y, MacDonald CC, Shenk T, Manley JL (February 1992). "The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs". Proceedings of the National Academy of Sciences of the United States of America. 89 (4): 1403–7. Bibcode:1992PNAS...89.1403T. doi: 10.1073/pnas.89.4.1403 . PMC 48459 . PMID 1741396.
Murthy KG, Manley JL (November 1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes & Development. 9 (21): 2672–83. doi: 10.1101/gad.9.21.2672 . PMID 7590244.
Takagaki Y, Manley JL (December 1994). "A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein". Nature. 372 (6505): 471–4. Bibcode:1994Natur.372..471T. doi:10.1038/372471a0. PMID 7984242. S2CID 4329672.
Rüegsegger U, Beyer K, Keller W (March 1996). "Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors". The Journal of Biological Chemistry. 271 (11): 6107–13. doi: 10.1074/jbc.271.11.6107 . PMID 8626397.
Kleiman FE, Manley JL (September 1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50". Science. 285 (5433): 1576–9. doi:10.1126/science.285.5433.1576. PMID 10477523.
Takagaki Y, Manley JL (March 2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component". Molecular and Cellular Biology. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. PMC 85326 . PMID 10669729.
Fong N, Bentley DL (July 2001). "Capping, splicing, and 3' processing are independently stimulated by RNA polymerase II: different functions for different segments of the CTD". Genes & Development. 15 (14): 1783–95. doi:10.1101/gad.889101. PMC 312735 . PMID 11459828.
Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (January 2002). "Directed proteomic analysis of the human nucleolus". Current Biology. 12 (1): 1–11. Bibcode:2002CBio...12....1A. doi: 10.1016/S0960-9822(01)00650-9 . PMID 11790298. S2CID 14132033.
Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ (January 2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nature Biotechnology. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
Tsuzuki M, Wu W, Nishikawa H, Hayami R, Oyake D, Yabuki Y, Fukuda M, Ohta T (February 2006). "A truncated splice variant of human BARD1 that lacks the RING finger and ankyrin repeats". Cancer Letters. 233 (1): 108–16. doi:10.1016/j.canlet.2005.03.012. PMID 15878232.

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[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000101138 – Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000027498 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid1358884-5] Takagaki Y, Manley JL (November 1992). "A human polyadenylation factor is a G protein beta-subunit homologue". The Journal of Biological Chemistry. 267 (33): 23471–4. doi: 10.1016/S0021-9258(18)35861-7 . PMID 1358884.

[pmid11257228-6] 1 2 Kleiman FE, Manley JL (March 2001). "The BARD1-CstF-50 interaction links mRNA 3' end formation to DNA damage and tumor suppression". Cell. 104 (5): 743–53. doi: 10.1016/S0092-8674(01)00270-7 . PMID 11257228. S2CID 15022237.

[entrez-7] 1 2 "Entrez Gene: CSTF1 cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa".

[pmid10477523-8] Kleiman FE, Manley JL (September 1999). "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50". Science. 285 (5433): 1576–9. doi:10.1126/science.285.5433.1576. PMID 10477523.

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CSTF1

Contents

Interactions

Related Research Articles

References

External links

Further reading